Action Potentials ; drug effects ; Animals ; Estradiol ; pharmacology ; Female ; Guinea Pigs ; Heart Ventricles ; cytology ; Myocardial Contraction ; drug effects ; Papillary Muscles ; drug effects ; physiology ; Progesterone ; pharmacology

OB JECTIVE: To investigate the chemical constituents from the fermented rice substrate of endophytic fungus Xylaria sp. HCCB03890. METHODS: The compounds were isolated with silica gel column chromatography and reversed-phase high performance liquid chromatography. The structures of these compounds were elucidated through MS and NMR spectroscopic analysis. The anti-microbes activities were evaluated by disk diffusion method, cytotoxic activities were determined by MTT method, and anti-HIV activities were measured by using a firefly luciferase assay system. RESULTS: Six compounds were isolated and identified as TV-acetyltyra-mine(1), schizostatin(2), piliformic acid(3), stigmast-5,22-dien-3-ol(4), dankasterone(5) and demethylincisterol(6) on the basis of spectroscopic data analyses. Compound 3 showed moderate anti-microbial activity. Compounds 4-6 exhibited cytotoxic and anti-HIV activities, especially, and compund 5 and 6 showed significant anti-HIV activities with IC50 values of 0.4 and 0.9 μg · mL, respectively. CONCLUSION: Compounds 2, 5 and 6 were for the first time isolated from Xylaria sp., moreover, 5 and 6 are for the first time found in terrestrial microbes.

ObjectiveTo observe the effect and side effect of intravascular He-Ne laser irradiation (ILIB) on the abnormal lipid metabolism induced by clozapine.Methods52 patients with schizophrenia (34 males, 18 females) took clozapine alone at a dosage of 200-350 mg/d. All patients were treated with ILIB every other day, 60 min once, ten times altogether. Before and after treatment, lipid metabolism, liver, kidney and blood coagulate functions of patients were tested.ResultsTC, TG, LDL-ch levels of patients decreased obviously after ILIB treatment (P<0.05), while HDL-ch level increased obviously (P<0.01). The liver, kidney and blood coagulate functions were as same as before treatment.ConclusionILIB has an extreme effect on the abnormal lipid metabolism induced by clozapine, and it is quite safe and has clinical practice value.

ObjectiveTo explore the effect of music treatment on memory decrease causing by clozapine of patients with schizophrenia. MethodsThe 70 patients with schizophrenia aged 18 to 30, who were in hospital for the first time and had graduated from middle school were divided into the experimental group who received music treatment and the control group with 35 cases each at random,when they reached the clinical cure standard after only taking clozapine. The two groups were given the same test before and after the experiment.ResultsThe memory measurement of the two groups had no difference before the experiment(P>0.05). The patients in the experimental group greatly increased their memory after the treatment (P<0.01), but these was no significent difference in the control group (P>0.05).Conclusions Music treatment can help to improve the memory of the patients received clozapinetreatment.

Animals ; Anti-Inflammatory Agents ; pharmacology ; Asthma ; pathology ; physiopathology ; Budesonide ; pharmacology ; Eosinophils ; drug effects ; pathology ; Inflammation ; pathology ; physiopathology ; NF-kappa B ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Respiratory System ; pathology

Humans ; Laparoscopy ; methods ; trends ; Pancreaticoduodenectomy ; methods

Objective HupA is one of the potential drugs which can be used to treat Alzheimer's disease(AD).The aim of this study was to explore the pharmacokinetics and biodistribution of HupA in vivo by using 11C-HupA.Methods A total of 25 SD rats were studied.They were divided into 5 groups (5 rats in each group).All had intravenous injection of 22 MBq(in0.2 ml)11C-HupA through tail vein.Dynamic im-aging Was acquired from 5 to 90 minutes after injection.Venous blood and organ activities were collected at 5,15,30,60.and 90 minutes after injection.Percentage activity of injected dose per gram of tissue(%ID/g)was calculated to characterize the biodistribution of tracer in different brain regions: frontal,apical, temporal,occipital,cerebellum,hippocampus,striatum,thalamencephalon, and brain stem, Variance analysis using SPSS 11.5 software was performed and compared among the study groups.Results 11C-HupA was character-istic for its quick clearance from blood,with half time T1/2 of (14.61±1.77) min,and clearance rate (CL)macokinetics of 11C-HupA in rats corresponded to a one-compartment model.with an activity curve(area 11C-HupA distribution in different brain regions,being greater in cerebral cortex,hippocampus,hypothala-mus and brain stem. Conclusions Pharmacokinetic study of 11C-HupA in brain was fast.convenient and showed high specificity and sensitivity.Its ability to quantitatively evaluate brain function and its character-istic distribution in mice provided some evidence for monitoring therapy in AD patients.

Background:Traditional tissue engineering methods to fabricate urinary tract patch have some drawbacks such as compromised cell viability and uneven cell distribution within scaffold.In this study,we combined three-dimensional (3D) bioprinting and tissue engineering method to form a tissue-engineered urinary tract patch,which could be employed for the application on Beagles urinary tract defect mode to verify its effectiveness on urinary tract reconstruction.Methods:Human adipose-derived stem cells (hADSCs) were dropped into smooth muscle differentiation medium to generate induced microtissues (ID-MTs),flow cytometry was utilized to detect the positive percentage for CD44,CD105,CD45,and CD34 of hADSCs.Expression of vascular endothelial growth factor A (VEGFA) and tumor necrosis factor-stimulated gene-6 (TSG-6) in hADSCs and MTs were identified by Western blotting.Then the ID-MTs were employed for 3D bioprinting.The bioprinted structure was encapsulated by transplantation into the subcutaneous tissue of nude mice for 1 week.After retrieval of the encapsulated structure,hematoxylin and eosin and Masson's trichrome staining were performed to demonstrate the morphology and reveal collagen and smooth muscle fibers,integral optical density (IOD) and area of interest were calculated for further semiquantitative analysis.Immunofluorescent double staining of CD31 and a-smooth muscle actin (α-SMA) were used to reveal vascularization of the encapsulated structure.Immunohistochemistry was performed to evaluate the expression of interleukin-2 (IL-2),α-SMA,and smoothelin of the MTs in the implanted structure.Afterward,the encapsulated structure was seeded with human urothelial cells.Immunofluorescent staining of cytokeradns AE1/AE3 was applied to inspect the morphology of seeded encapsulated structure.Results:The semi-quantitative assay showed that the relative protein expression of VEGFA was 0.355 ± 0.038 in the hADSCs vs.0.649 ± 0.150 in the MTs (t--3.291,P =0.030),while TSG-6 expression was 0.492 ± 0.092 in the hADSCs vs.1.256 ± 0.401 in the MTs (t =3.216,P =0.032).The semi-quantitative analysis showed that the mean IOD of IL-2 in the MT group was 7.67 ± 1.26,while 12.6 ± 4.79 in the hADSCs group,but semi-quantitative analysis showed that there was no statistical significance in the difference between the two groups (t =1.724,P =0.16).The semi-quantitative analysis showed that IOD was 71.7 ± 14.2 in noninduced MTs (NI-MTs) vs.35.7 ± 11.4 in ID-MTs for collagen fibers (t =3.428,P =0.027) and 12.8 ± 1.9 in NI-MTs vs.30.6 ± 8.9 in ID-MTs for smooth muscle fibers (t=3.369,P=0.028);furthermore,the mean IOD was 0.0613 ±0.0172 in ID-MTs vs.0.0017 ± 0.0009 in NI-MTs for α-SMA (t =5.994,P =0.027),while 0.0355 ± 0.0128 in ID-MTs vs.0.0035 ± 0.0022 in NI-MTs for smoothelin (t=4.268,P =0.013),which indicate that 3D bioprinted structure containing ID-MTs could mimic the smooth muscle layer of native urinary tract.After encapsulation of the urinary tract patch for additional cell adhesion,urothelial cells were seeded onto the encapsulated structures,and a monolayer urothelial cell was observed.Conclusion:Through 3D bioprinting and tissue engineering methods,we provided a promising way to fabricate tissue-engineered urinary tract patch for further investigation.

Atrial Fibrillation ; therapy ; Catheter Ablation ; adverse effects ; Female ; Humans ; Pulmonary Veins ; Venous Thrombosis ; etiology

Animals ; Arsenic ; toxicity ; Comet Assay ; DNA Damage ; drug effects ; Lymphocytes ; drug effects ; metabolism ; Male ; Malondialdehyde ; metabolism ; Rats ; Rats, Wistar ; Tobacco Smoke Pollution ; adverse effects