Dental Research ; Humans ; Tooth Bleaching

Cochlear Implantation ; Cochlear Implants ; Humans ; Tinnitus

Adult ; Churg-Strauss Syndrome ; complications ; Humans ; Male ; Myocardial Infarction ; complications

objective: To investigate the effects of cerium oxide content on the color of alumina glass composite. Methods: Infiltration glass powder with different content of cerium oxide (1%～4%) were fabricated. Alumina glass composite was prepared by infiltrating molten glass into presintered alumina block at 1 250 ℃ for 2 h, and the color of the alumina glass composite were measured with Minolta chromatic instrument(CR 321) . For comparison, the color of VITA In Ceram alumina technical shade guide was recorded too. Results: After infiltration, the b * range of the alumina glass composite was in accord with that of the shade guide. With the increasing content of the cerium oxide, a *, b * and chroma increased, L * decreased, the hue of the alumina glass composite shifted from yellow green to yellow red. Conclusion: Cerium oxide may effectively enhance b * of the alumina glass composite, but its ability of reducing L * and enhancing a * is weak.

Objective: To measure the color of dental CAD/CAM alumina-glass-composites(AGC). Methods: Dental CAD/CAM alumina block were infiltrated at 1 120 ℃ by laboratory made AG1,AG2,AG3 and AG4 color serials of infiltration glass powder and Vita In-Ceram Alumina AL1,AL2,AL3 and AL4 infiltration glass respectively. Specimens were made with the area of 10 mm?10 mm,thickness of 0.5,1 and 1.5 mm respectively. Color parameters were measured by Minolta CM-2600d spectrophotometer. Results:The specimen with the thickness of 1 mm was used as the standard for color measurement.After infiltration with AL1-AL4 glass powder,the color parameters of Vita alumina core ceramic were L*: 69.39-78.41,a*:1.82- 4.02,b*:18.35-24.42,when infiltrated with AG1-AG4 glass powder, the color parameters of CAD/CAM AGC were L*:68.80-78.44,a*:1.32- 4.75,b*:16.85- 21.86. b*value and chroma of the AG3 core ceramic were lower than that of AL3 core ceramic,while a* value was higher.b* value and chroma of the AG1 and AG2 core ceramic were lower than that of AL1 and AL2 core ceramic. The reflectance curve of CAD/CAM AGC infiltrated by AG1-AG4 glass powder demonstrated the similar trend as that of the core ceramic infiltrated by Vita In-Ceram glass powder. Conclusion: The color range and surface reflection rate of laboratory made AG1-AG4 AGC are similar with those of Vita AL1-AL4 In-Ceram Alumina core and may meet the need of color match with veneer ceramics.

Aged ; Anthracosis ; physiopathology ; Autonomic Nervous System ; physiopathology ; Death, Sudden ; Humans ; Male ; Middle Aged

Objective To investigate the destructive effect of cadmium (Cd)and the protective effect of astragaloside ( Asd) on TM3 cells.Methods The best protective concentration of Asd on TM 3 cells after Cd treatment was selected by MTT cell viability experiment .The apoptosis rate of TM3 cells was measured by flow cytometry .The expression of connexin 43(Cx43) was detected by immunohistochemistry .The morphological changes of TM3 cells were observed by electron mi-croscopy .Results The viability of TM3 cells in both control and Asd-treated group was not significantly different after 24 h or 48 h,but it was significantly decreased in Cd group .However , the viability of TM3 cells in Cd +As group was higher than that in Cd group .Flow cytometry showed that there was no significant difference in the apoptosis rate of TM 3 cells be-tween the control and Asd-treated group after 24 h,but it was obviously increased with the Cd concentration .The apoptosis rate of Cd+Asd group was lower than that in Cd group (P<0.01).Immunohistochemistry demonstrated that the average optical density (AOD) of the positive product of Cx43 in Cd-treated TM3 cells was obviously decreased (P<0.01), but the average gray value (AGV) was significantly increased when compared with the control group (P<0.01).The ultra-structural changes in TM3 cells were obvious after Cd treatment ,but those in Cd+Asd group were improved when compared with Cd group.Conclusion Cd reduces the expression of Cx 43 and damages the morphology of TM 3 cells.Asd has protec-tive effect on Cd-induced TM3 cell injury.

Objective To explore the effects of hyperglycemia and oxidized low density lipoprotein (ox-LDL) on the differentiation of macrophage derived THP-1 monocytes. Methods THP-1 human monocytic leukemia cell line was cultured in vitro, and the differentiation of THP-1 cells into macrophages was induced by phorbol esters. The macrophages were then incubated with the absence of D-glucose and ox-LDL (control group), 30 mmol/L D-glucose (hyperglycemia group), 100 μg/mL ox-LDL (ox-LDL group) or 30 mmol/L D-glucose and 100 μg/mL ox-LDL(G-ox-LDL group) for 24 h. High performance liquid chromatography was used for qualitative and quantitative analysis of intracellular cholesterol and cholesteryl esters. Both light microscope with red oil O staining technique and transmission electron microscope were employed to observe the morphology of treated and control THP-1 cells. Results A large number of intracellular red oil O stained granules and lipid vacuoles were observed in ox-LDL group and G-ox-LDL group, the contents of total cholesterol and cholesteryl esters were significantly higher than those of control group (P<0.05), and the contents of cholesteryl esters were higher than 50% of total cholesterol in both groups. However, only a few intracellular red oil O stained granules and lipid vacuoles were observed in control group and hyperglycemia group, there was no significant difference in the contents of total cholesterol and choleateryl esters between control group and hyperglycemia group (P>0.05), and the contents of cholesteryl esters were less than 50% of total cholesterol in both groups. Conclusion Foam cells form when THP-1 cells are incubated with ox-LDL, while hyperglycemia alone can not convert THP-1 cells to foam cells, indicating that ox-LDL is necessary for the macrophages derived THP-1 monocytes to turn into foam cells.

Objective A HPLC method was established for determination of the content of ATST in the aqueous humor of rabbits. MethodsThe mobile phase was consisting of methanol-1％ triethylamine(57:43) and omeprazole (OMZ) as internal standard. The detection was carried out with an ultraviolet detector operated at 235nm. ResultsThere was linearity over the range of 2. 056～41.12 ug/ml in the humor aquosus, r=0.9997. The average recovery of ATST was 94.58 ％. Intra-day and in- ter-day RSD were less than 5 ％ and 10 ％ ( n = 5), respectively. Conclusion The method is reliable. It can be used for the study on the pharmacokinetics of ATST.

Forty-eight patients with subclinical Cushing's syndrome(SCS)were evaluated.Eleven of them underwent adrenalectomy(Group 1)and the other 37 cases did not(Group 2).Serum and urine corticosol, plasma ACTH and parameters related to metabolic syndrome(such as waist circumference,blood pressure,blood lipids and fasting plasma glucose)were measured.The data at diagnosis were compared with those during the survey.The results indicated that patients with SCS had a significantly high prevalence of metabolic syndrome.The symptoms and signs of metabolic syndrome could be improved after removing the tumor.Otherwise there is no improvement,some patients will even develop into overt Cushing's syndrome.