2.Roles of immunopathological technique in diagnosis of renal diseases.
Chinese Journal of Pathology 2006;35(9):513-516
Complement C3
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metabolism
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Fluorescent Antibody Technique
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Glomerulonephritis, IGA
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diagnosis
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metabolism
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Glomerulonephritis, Membranous
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diagnosis
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metabolism
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Humans
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Immunoglobulin G
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metabolism
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Immunohistochemistry
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methods
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Kidney
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chemistry
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pathology
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ultrastructure
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Kidney Diseases
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diagnosis
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metabolism
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Microscopy, Electron
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Nephritis
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diagnosis
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metabolism
3.Primary hypophysitis
Li ZANG ; Yi-Ming MU ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Primary hypophysitis includes 3 subtypes:lymphocytic,granulomatous and xanthomatous hypophysitis. The primary hypophysitis has a low morbidity and the final diagnosis is mostly determined based on the pathological findings after operation.Now it is considered as an organ specific autoimmune disease.Its clinical manifestations and imaging features are similar to hypophyseal tumor,but the endocrine and pathologic features are different.Glucocortieoid pulse therapy and surgery are the two methods of treatment.
4. Preparation of paclitaxel-loaded mixed micelles and its in situ absorption in rat intestine
Chinese Pharmaceutical Journal 2013;48(13):1076-1082
OBJECTIVE: To study the oral absorption of paclitaxel-loaded mixed micelles made of D-α-tocopherol polyethylene glycol 1000 succinate(TPGS) and sodium cholate(NaC) in rats. METHODS: Paclitaxel-loaded mixed micelles were prepared by film dispersion method. The Zeta potential and diameter distribution of TPGS/NaC mixed micelles were measured using laser size scattering determinator. The morphology of micelles was observed by transmission electron microscope. Dialysis method was used to evaluate the release behavior of drug-loaded micelles in vitro. The absorption kinetics was obtained by in situ perfusion method in rats. RESULTS: Most of the mixed micelles were spherical with an average diameter of 24.2 nm and the Zeta potential was -7.84 mV. Compared to the bulk drug, the apparent absorption rate constant (Ka)of paclitaxel-loaded mixed micelles was increased significantly. CONCLUSION: TPGS/NaC mixed micelles can improve the oral absorption of paclitaxel and increase its oral bioavailability.
5.Efficacy of erlotinib on advanced non-small-cell lung cancer
Cancer Research and Clinic 2011;23(8):509-511
Objective To evaluate the efficacy of erlotinib on advanced NSCLC, and observe its adverse events. Methods An open labeled, expanded access program (EAP) was conducted on 19 pathologically confirmed advanced NSCLC patients who had received at least one regimen chemotherapy.Erlotinib (150 mg) was orally administered daily till disease progression or intolerable adverse events developed.The efficacy was evaluated according to RECIST criteria; the adverse events were evaluated according to NCI criteria.Results In 19 patients, the objective response rate was 21.1% (4/19), and the disease control rate was 84.2 % (16/19); the median progression-free survival time was 7.5 months (3-36 months), and the median survival time was 15.9 months (9-39 months).Adverse events were generally mild (grade Ⅰ or Ⅱ ), including skin rash (84.2 %) and diarrhea (57.9 %). One (5.3 %) patient developed grade Ⅲ elevation of serum glutamate pyruvate transaminase.No grade Ⅳ drug-related adverse event occurred.Conclusion Erlotinib is effective and safe for locally advanced or metastatic NSCLC patients who have failed previous chemotherapy.
6.Erythropoietin and neonatal brain injury.
Chinese Journal of Pediatrics 2011;49(10):756-760
7.Hypoxia-inducible factor-1α and small ubiquitin-related modifier
International Journal of Pediatrics 2010;37(2):116-118
Hypoxia-inducible factor-1 (HIF-1) is a key transciptional regulator of cellular and systemic oxygen homeostasis,composed of the two subunits,HIF-1α and HIF-1β.HIF-1α is an inducible subunit regulated by hypoxia,which is posttranslationally modified by various ways,including ubiquitination,phosphorylation,hydroxylation,acetylation and glycation,accordingly affecting its protein's stability,nuclear translocation and regulation of its target genes.Small ubiquitin-related modifier (SUMO) is an micromolecule protein participating in posttranslational modifications of proteins dynamicly in eukaryotic organism,which is similar in structure to ubiquitin.SUMOylation and deSUMOylation are involved in multiple regulations of biologic processes,such as signal transduction,transcriptional regulation,cell cycle processes and biological rhythm.SUMOylation is related to HIF-1α stability and transcription activity in hypoxia,providing a newthread to study self-regulation mechanism of HIF-1α for us.
8.Role and mechanism of telomerase reverse transcriptase in ischemic brain damage
International Journal of Cerebrovascular Diseases 2010;18(9):706-710
Objective Telomerase reverse transcriptase (TERT) participates in pathologic processes of many ischemic damages.It also plays an important role in ischemic brain damage.There are a lot of activation mechanisms of TERT after cerebral ischemia.At present,the hypoxia-inducible factor-1 has been more clearly studied.The protective effect of TERT in ischemic brain damage may be associated with anti-apoptosis,promoting cell survival,promoting angiogenesis,and protecting mitochondria.
9.Relationships among the expression of VEGF, MMP-2 , MMP-9 and metastasis in breast carcinoma
Junwei ZHANG ; Yi ZHANG ; Changzheng MU
Journal of Chinese Physician 2010;12(11):1490-1493
Objective To study the expression of VEGF, MMP-2 and MMP-9 in benign and malignant lesions of breast and their effects on breast carcinoma's invasiveness and metastasis. Methods VEGF,MMP-2 and MMP-9 expression was detected by SP method in 20 breast fibroadenoma and 12 normal breast specimens taken from non-cancerous regions adjacent to breast cancer tissue and 38 breast carcinomas. Results The expression rate of VEGF, MMP-2 and MMP-9 in breast carcinoma was remarkably higher than those in normal breast tissue and breast fibrogdenoma (63.2% vs 25.0% and 30. 0% ,78. 95% vs 33.3% and 35.0% and 71.1% vs 41.7% and 45.0% ,respectively; P <0.05). There was a correlation between VEGF, MMP-2 and MMP-9 expression and nodal metastasis ( P <0.05). High expression of VEGF, MMP-2 and MMP-9 was correlated with pathological grade ( P < 0.05, respectively). The overexpression of VEGF was related with those of MMP-2 and MMP-9 in breast cancer( VEGF and MMP-2:rs =0.541, P <0.01 ;VEGF and M M P-9: rs = 0.11, P < 0.01; MMP-2and M MP-9: rs= 0P < 0.01 ). Conclusion VEGF,MMP-2 and MMP-9 overexpression is strongly related to the invasiveness and metastasis of breast carcinoma, which might be valuable in evaluating tumor invasion and metastasis.
10.Emphasis should be placed on the diagnosis and therapy of tumor induced osteomalacia.
Chinese Medical Journal 2011;124(2):163-165
Humans
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Hypophosphatemia
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blood
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complications
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Neoplasms
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blood
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complications
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Osteomalacia
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blood
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diagnosis
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drug therapy
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etiology
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surgery
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Phosphates
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blood