1.Basic principles of selecting the site of blood reserve bank for preparedness against war
Yuxiu LIU ; Jun ZHENG ; Xiaoli XU ; Jianfeng LUAN ; Baolin YANG ; Yurong WANG ; Xinyong HU ; Xueming YI
Journal of Medical Postgraduates 2003;0(10):-
The blood reserve bank for preparedness against war,as a store of blood products and a base of blood supply,bears the responsibility of wartime emergency blood supply.Site selection is the basis of the construction of the blood reserve bank and directly related with its capacity of blood supply in both peace and wartime.The decision-making process concerning site selection is complicated and multi-aimed with various factors mutually related and conditioned.This paper proposes some basic principles of selecting the site of the blood reserve bank for preparedness against war,which are hoped to be beneficial for the demonstration of its site selection.
2.Treatment for duodenal fistula by enteric catheter fluid closuring combined with self-made double cannula rinse and drainage.
You Guo DAI ; Jia Xin WANG ; Da Fu ZHANG ; You Yi LIU ; Yu LYU ; Yi Bo HU ; Xiao HAN ; Li Kun LUAN ; Qin LIU ; Zhen Hui LI
Chinese Journal of Gastrointestinal Surgery 2021;24(8):718-721
3.A case report on the relationship between treatment-resistant childhood-onset schizophrenia and an abnormally enlarged cavum septum pellucidum combined with cavum vergae.
Zheng-luan LIAO ; Shao-hua HU ; Yi XU
Chinese Medical Journal 2012;125(7):1349-1351
The treatment of refractory schizophrenia has been a clinical challenge for most psychiatrists; the possible reasons include diagnostic errors, medical conditions and brain dysgenesis. Here, we described a patient with childhood-onset schizophrenia who had severe psychiatric symptoms such as auditory hallucinations and persecutory delusions, and etc. We reexamined all his possible medical conditions and found that the patient had an abnormally enlarged cavus septum pellucidum (CSP) combined with cavum vergae (CV) (maximum length >30 mm). Some reports suggested that abnormal CSP (length >6 mm) has a significant association with schizophrenia. However, abnormally large CSP or CSP/CV and related prognosis were reported rarely. This case suggested that abnormally enlarged CSP or CSP/CV may worsen the prognosis.
Adolescent
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Antipsychotic Agents
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therapeutic use
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Benzodiazepines
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therapeutic use
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Clozapine
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therapeutic use
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Dibenzothiazepines
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therapeutic use
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Humans
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Male
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Quetiapine Fumarate
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Schizophrenia, Childhood
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diagnosis
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drug therapy
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pathology
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Septum Pellucidum
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pathology
4.The expression difference between estrus and non-estrus of ERβmRNA of beagles by real-time quantitative PCR
Rui ZHONG ; Yi GAN ; Xiumei REN ; Qin XU ; Yanbin ZHAO ; Bing LIU ; Zhaozeng SUN ; Yujing ZHU ; Xinhong LUAN ; Zhongming HU ; Yong ZHANG ; Lin ZENG
Chinese Journal of Comparative Medicine 2014;(11):15-19
Objective To quantitative the changing information of estrogen receptor βgene which was in tissue and organ of sex gland during oestrus and dioestrus of Beagles, and to show the different expression situation of hypothalamus-pituitary-gonad axis during oestrus and dioestrus, and providing the basic of theory to research deeply the mechanism of heat of Beagles. Methods As the key gene in regulation reproduction, ERβgene is located in hypothalamus-pituitary-gonad axis, so using Beagles which was in oestrus and dioestrus, and extract the RNA from hypothalamus、pituitary、ovary and uterus respectively,after reverse transcription we detected the expression of ERβgene by real-time quantitative PCR.Results The expression of ERβgene mRNA from ovary、uterus、pituitary、hypothalamus of Beagles which was in dioestrus was 0.35 times, 0.17 times, 0.44 times and 0.43 times than the expression of ERβgene mRNA from ovary, uterus, pituitary, hypothalamus of Beagles which was in oestrus.Conclusion The expression of ERβgene was up-regulation in hypothalamus-pituitary-ovary axis of Beagles which was in oestrus.
5. Retrospective analysis of clinical characteristics and prognosis of patients with sepsis related liver injury
Ying CAO ; Zheng-gang LUAN ; Liang WANG ; Yi-na LIU ; Bo HU ; Xiao-chun MA
Chinese Journal of Practical Internal Medicine 2019;39(02):163-167
OBJECTIVE: To analyze the incidence, clinical characteristics and prognosis of sepsis-related liver injury(SELI). METHODS: The data of septic patients in the department of Critical Care Medicine of the First Affiliated Hospital of China Medical University from January 2013 to December 2014 were collected and the basic information, infection site, emergency operation and residence were recorded. Also, the duration of ICU, the first 24 h APACHE II and SOFA score, mechanical ventilation, MODS, nutritional support, serum total bilirubin and aminotransferase, the duration of liver dysfunction and the duration of liver dysfunction during ICU stay were recorded. Rank sum test was used to compare age, APACHE II score, SOFA score, length of stay in ICU, duration of mechanical ventilation; Chi square test was used to compare the patient's source, type of operation, whether MODS, and nutritional support; The logistic regression analysis was used to analyze the related factors of death. RESULTS: A total of 341 septic patients with sepsis were included in the study, including 96 patients with septis-related liver dysfunction. The main manifestations were elevated transaminase in 17 cases(17.71%), elevated bilirubin in 31 cases(32.29%), elevated bilirubin and transaminase in 48 cases(50%) and liver injury in 66 cases(68.75%) of sepsic patients occurred within 1-3 days of ICU 3.22 days(1-40 days). The morbidity and mortality of MODS in sepsis-related liver injury patients were 70.83% and 33.33%. Statistically differences in APACHE II scores, SOFA scores, mechanical ventilation duration, and MODS in patients with different prognosis were revealed. Further logistic regression analysis showed that sepsis with MODS was an independent risk factor for death. There were statistical difference in duration of symptoms, duration of ICU stay and prognosis. CONCLUSION: The morbidity of SELI is high, mostly in the early stage of sepsis, men are more prone to the disease. The abdominal cavity is the most common site of infection. High APACHEII score, prolonged mechanical ventilation and MODS are independent risk factors for SELI.
6.A survey of mental health among transferred injured survivors in Wenchuan earthquake in Sichuan.
Ning WEI ; Wei-hua ZHOU ; Jian-bo HU ; Man-li HUANG ; Hong-li QI ; Shao-hua HU ; Ying-ran ZHANG ; Zheng-luan LIAO ; Yi XU
Chinese Journal of Preventive Medicine 2009;43(5):376-379
OBJECTIVETo assess the depression and anxious status among transferred injured survivors in Wenchuan earthquake in Sichuan province.
METHODSA total of 43 transferred injured survivors were investigated by questionnaire exploring their trauma symptoms and mental health status.
RESULTSHigh rates of trauma symptoms were remarkably observed in these survivors. Of all the respondents, 60% had some emotional symptoms and sleeping difficulties. About one third of respondents experienced recurrent and intrusive distressing recollection of event, 16 (37.21%) experienced nightmare, 15 (34.88%) had flashback and 7 (16.28%) of them tried to avoid relative stress.
CONCLUSIONMany mental symptoms were observed in transferred injured survivors. The two major factors of mental stress were emotional symptoms and re-experience of the disaster.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Disasters ; Earthquakes ; Female ; Humans ; Male ; Middle Aged ; Stress Disorders, Post-Traumatic ; psychology ; Stress, Psychological ; Survivors ; psychology ; statistics & numerical data ; Wounds and Injuries ; psychology ; Young Adult
7.Construction of the stable expression system of NIH3T3 fibroblast with pcDNA3.1(-)-hTGFbeta3 and the study on the proliferation of the system fibroblasts.
Shi-Jie TANG ; Si-Tian XIE ; Su-Luan HU ; Zhi-Qiang XIAO ; Ji-Kui SHEN ; Hong YI
Chinese Journal of Plastic Surgery 2006;22(2):109-112
OBJECTIVETo construct and identify the stable expression system of NIH3T3 fibroblast with eukaryotic expression vector of human transforming growth factor beta3 (pcDNA3.1 (-)/TGFbeta3). So as to investigate the proliferation of NIH3T3 fibroblasts transfected with hTGFbeta3 gene stably.
METHODSThe stable transfection of NIH3T3 fibroblasts with recombinant plasmid expressing hTGFbeta3 was established by using LipofectamineTM2000 and G418 selection. The mRNA and protein expression of TGFbeta3 were detected by the RT-PCR and Western blot method, respectively. Microscope and MTT were adopted to examine the proliferation of the stable expression system of fibroblasts with hTGFbeta3.
RESULTSAfter G418 selection, RT-PCR and Western blot analysis, 7 out of 10 cell lines transfected with pcDNA3.1 (-)/TGFbeta3 expressed with very high level of TGFbeta3, as compared with vector control transfectants that showed no expression, and compared with the other cell lines that expressed relatively low level. The stable transfection of NIH3T3 fibroblasts growth slowed down significantly (P < 0.05).
CONCLUSIONThe stable expression system of NIH3T3 fibroblast with hTGFbeta3 were constructed successfully. The TGFbeta3 gene could inhibit the proliferation of NIH3T3 fibroblasts in vitro.
Animals ; Cell Proliferation ; Fibroblasts ; metabolism ; Humans ; Mice ; NIH 3T3 Cells ; Plasmids ; Transfection ; Transforming Growth Factor beta3 ; genetics ; metabolism
8.Effect of highway transportation on the quality of red blood cells.
Gen-Hong YAO ; Jian-Feng LUAN ; Dong YE ; Pei-Yuan ZHU ; Jing-Mei YAN ; Ping HONG ; Xin-Yong HU ; Xue-Ming YI
Journal of Experimental Hematology 2008;16(4):922-925
This study was purposed to investigate the effect of highway transportation on the quality of blood components so as to provide experimental basis to meet the needs of military operations. The transport condition was simulated by random vibration test. The red blood cells, leukocyte-reduced red blood cells, washed red blood cells were randomly vibrated (C Road) for 4 hours. Then, these blood components were stored in refrigerator for 15 days (4 degrees C). Six milliliters of blood were collected before vibration, after vibration, and at day 15 days of storage after vibration, respectively. The suspension was isolated. The free hemoglobin (FHb), routine hematological parameters, and biochemical indexes were determined. The results showed that FHb, lactate dehydrogenase (LDH), K(+) of red blood cells and leukocyte-reduced red blood cells did not significantly change after vibration and storage. However, FHb, LDH and K(+) of washed red blood cells increased significantly after simulated transportation (p < 0.05). The levels of these parameters at day 15 of storage after vibration were also significantly higher than those after vibration (p < 0.01). The changes of other hematological parameters were not significant in three blood components after vibration (C Road) and storage for 15 day. In conclusion, red blood cells and leukocyte-reduced red blood cells were qualified for clinic transfusion even after transportation within 4 hours for 15 day storage later, if they were kept in proper blood container and protected from damping. However, the washed red blood cells could not be used for clinic after similar transport in the military operations.
Blood Preservation
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Cryopreservation
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Erythrocytes
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chemistry
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Humans
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L-Lactate Dehydrogenase
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blood
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Transportation
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Vibration
9.Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene.
Xiang-ling LUAN ; Wei KONG ; Su-jun LIU ; Li LEI ; Yan HU ; Jun HOU ; Hong-hui SHEN ; Yi-chen WU ; Shao-li YOU ; Pan-yong MAO ; Shao-jie XIN
Journal of Southern Medical University 2008;28(2):252-254
OBJECTIVETo construct the virus-like parcel expressing hepatitis B virus (HBV) C gene and identify its immunogenicity.
METHODSHBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara (MVA).
RESULTSpSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immunogenicity.
CONCLUSIONThe MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
Genes, Viral ; Genetic Vectors ; Hepatitis B Core Antigens ; genetics ; Recombination, Genetic ; Vaccinia virus ; genetics
10.Vascular endothelial growth factor expressing mesenchymal stem cells improves cardiac function in chronic myocardial infarction in pigs.
Fu YI ; Wen-yi GUO ; An-lin LÜ ; Hai-chang WANG ; Hu LI ; Wei-jie LI ; Bing LIU ; Dian-xin ZHANG ; Rong-hua LUAN ; He-xiang CHENG ; Fei LI ; Tao QIN ; Zhi-jing ZHAO ; Feng GAO ; Guo-liang JIA
Chinese Medical Journal 2006;119(19):1664-1668