Objective To study the safety and efficiency of transurethral electrochemical therapy (ECT) on benign prostatic hyperplasia (BPH). Methods An experimental electrechomical therapy on prostate has been carred out in 12 dogs.The prostate tissue and the nearly organs were studied for any morphological changes.On the basis of the experiment,ECT was then carried out for 11 patients suffered from BPH.The relevant parameters were assessed. Results In the experimental group,good therapeutic effect was achieved if the electrode was 10 mm shorter than the prostatic urethra.On the other hand if the electrode was 10 mm longer than the prostatic urethera,urethro-rectal fistula and urine incontinence might result.In the clinical group,the effective rate was 82% with no complication observed.Before the treatment RI was 0.70?0.04 and 2 weeks after treatment 0.73?0.03;IPSS 26.7?6.2 and 19.5?4.7;QOL 4.9?0.5 and 3.5?0.6;Qmax (7.8?2.0) and (12.4?3.1)ml/s;PVR (129.1?40.8) and (64.1?27.4)ml;V (48.0?13.1) and ( 47.0?13.5)ml.There was significant difference of IPSS,QOL,Qmax,PVR,RI before and after treatment (P0.05). Conclusions With proper maniputation and adequate length of the electrode used,ECT is a safe,efficient and mini-invasive means for treating BPH in high risk patients nontolerable to open surgery.

Objective To investigate the expression of Yes‐associated protein(YAP) in human papillary thyroid cancer and its influence on cell growth .Methods The samples in 57 cases of papillary thyroid cancer treated by operation resection in the gen‐eral surgery department of this hospital and the matched tumor‐adjacent tissues were collected .All the cases were definitely diag‐nosed by the pathology examination .The expression of YAP protein in the cancer tissue and corresponding tumor‐adjacent tissue were determined by the immunohistochemistry(IHC)staining .The relationship between the YAP protein and the clinicopathological data was statistically analyzed .siRNA was used to silence the expression of YAP in B‐CPAP cells ,MTT and flow cytometry were used to measure the proliferation and apoptosis changes .Results The expression level of YAP was markedly higher in papillary thyroid cancer tissues than in tumor adjacent tissues (P<0 .05);moreover the expression of YAP protein was positively correlated with the tumor size and TNM stage (P<0 .05) .Silencing YAP gene could significantly inhibit the cell proliferation ability and pro‐mote cell apoptosis(P<0 .05) .Conclusion YAP is highly expressed in papillary thyroid cancer tissues and is related with its ad‐verse clinicopathological characteristics ,down‐regulating YAP gene can significantly inhibit the cell growth .

Objective To evaluate the surgical treatment of huge staghorn stones. Method The clinic dates of 184 cases treated with an incision of the infrarenal sinus supplemented by a postrenal low pole segmental incision were studied. Result 184 cases were all successful.Conclusion We conclude that this method, need no interruption of renal blood flow had advantages of simple procedure less bleeding, completely removing calculi, protecting renal function.

Purpose: To study the expression of VEGF, VEGF-C, fit-4 in breast cancer and its relationship with lymph node metastasis and explore the significance of lymphangiogenesis on metastasis via lymphtics. Methods: Paraffin-embedded specimens from 82 patients with primary breast cancer who had undergone radical mastectomy with lymph node dissection were studied. VEGF, VEGF-C, flt-4 expression were investigated by immunohistochemical staining. Results: Positive staining for VEGF, VEGF-C, flt-4 were observed in some breast cancer cells. Furthermore, the expression of VEGF-C, flt-4 were significantly higher in lymph node-positive group than in node-negative group. Correlation was found within the expression of VEGF-C, flt4 also. Conclusions: Clinical significance was found between VEGF-C, flt-4 expression and lymph-node metastasis in breast cancer, and may be involved in the metastatic process.

Liver fibrosis can be caused by chronic liver injury arising from various etiological factors.The activation of the hepatic stellate cell(HSC) is the central event in liver fibrosis,the apoptosis of which was the initiating factor for the reversal process.The apoptosis of HSC is very complicated.The signaling pathways lack of enough research in regulating and controlling and activating HSC's apoptosis.The apoptosis pathways as known currently mainly include mitochondrial pathway,death receptor pathway and non-death receptor pathway.Based on these signaling pathways,selectively inducing cell death of activated HSCs is becoming the new antifibrotic treatment.This review focuses on the new progress of HSC's apoptosis signaling pathways,and summarizes the drugs that have the effect of inducing HSC apoptosis.

Objective To identify the risk factors for the development of intraoperative hypothermia in the patients undergoing thoracic surgery.Methods One hundred twenty patients of both sexes,aged 23-83 yr,weighing 43-92 kg,of ASA physical status Ⅰ or Ⅱ,scheduled for elective thoracic surgery,who had an expected surgical duration of more than 2 h,with the core body temperature of 36.0-37.5 ℃,were included.After admission to the operating room,the core body temperature was measured.Intraoperative nasopharyngeal temperature of lower than 36 ℃ was defined as hypothermia.The patients were randomly divided into hypothermia group or non-hypothermia group according to whether or not intraoperative hypothermia occurred.Factors including the patient characteristics,the total amount of fluid infused (including the volume of blood transfused),duration and way of anesthesia and duration and type of surgery were recorded.The risk factors associated with intraoperative hypothermia were identified by logistic regression analysis.Results A total of 94 patients developed hypothermia during surgery,and the incidence of hypothermia was 78.3％.The lowest body temperature was 33.6 ℃.There was significant difference between the two groups in age,duration of surgery,the total amount of fluid infused,and the body temperature when patients were admitted to the operating room.Logistic regression analysis revealed that the total amount of fluid infused＞2 000 ml (OR =3.499) and low body temperature when patients were admitted to the operating room (OR =0.074) were independent risk factors for intraoperative hypothermia in the patients undergoing thoracic surgery.Conclusion The total amount of intravenous fluid infused＞2 000 ml and low body temperature when patients were admitted to the operating room are independent risk factors in the patients undergoing thoracic surgery.

Objective To observe the protective and repairing effects of bone mesenchymal stem cells(BMSCs) transplantation on cerebral infarction in rats and to study the different effect of transplantation at different time points. Methods Middle cerebral artery occlusion(MCAO) models were set up,and the rats were divided into a control group,a group with PBS transplantation and five groups with BMSCs transplantation 3,6,12,24 and 72 h after MCAO,respectively.The volume of infarction area and the neurological severity score(NSS) in all the groups were compared. Results Twenty-eight days after MCAO,the TTC staining indicated that the volume of infarction area in the groups with BMSCs transplantation decreased remarkably compared with the control group and the group with PBS transplantation(P

Objective: To search for a method for radical resection of pancreatic and duodena malignancy involving the mesentery root and for the long post-operation survival of patients. Methods: From Jan. 2004 to Aug. 2006, a total of 26 (16 male and 10 female, aged 27-70) patients with pancreatic and duodenal malignancy involving the mesentery root were treated in our department. The patients included 3 with duodenal malignancy and 23 with pancreatic malignancy. Curative resection was performed by the extended pancreaticoduodenetomy (Whipple procedure) combined with mesentery root resection (MRR) for all patients. The outcomes, safety and the post-operation survival rate were analyzed retrospectively. Results: Thirteen patients were treated with Whipple procedures combined with MRR, 9 were treated with partial portal vein/superior mesenteric vein (PV/SMV) and reconstruction of the vessel, and 4 patients received pre-shunt between PV and SMV with artificial vessel graft before the extended Whipple and NMR procedures. The operation time was 2. 5 to 7 (4. 4 ± 1. 1) hour 9 and blood loss was 300 to 5 000 (1 892 ± 1 414) ml with the blood transfusion of 0 to 5 600 (2 100 ± 1 586) ml. There was no death in our group and 7 (27%) had post-operation complication. The post-operation hospital stay was 10 to 30 days. The pathologic examination showed negative surgical margins for all specimens. The tumor size was 4 to 10 (6.17 ± 2.03) cm. After a follow-up of 9 to 38 months, the pain was relieved in all patients. One of the 3 patients with duodenal adenocarcinoma had liver metastasis at 10 months after operation, and the other 2 survived 10 months and 27 months without evidence of tumor reccurence. The patient with pancreatic micro-adenocarcinoma died of local reccurence 9 months after operation. The patient with neuroendocrine carcinoma died of organ failure 24 months after operation. The patient with lymphoma have survived for 24 months after operation. The 1-year and 2-year accumulated survival rates in the 20 cases with pancreatic ductal cancer were 86.6% and 45.6%, respectively. Conclusion: The extended Whipple procedure with MRR is safe and effective. It can obtain RO resection in patients with malignant tumors (over 5 cm in diameter) in the head, neck and uncinate process of the pancreas and duodenal.

ObjectiveTo explore the diagnostic value of 16-slices CT in urinary calculus. Methods25 cases of suspected urinary calculus were examined by 16-slices CT and some of these images were reconstructed with MPR. ResultsIn the 25 casces CT found 21cases of urinary calculus,among them kidney calculus 22 cases,urinary tract calculus 13 cases.In 5 of 21 cases,urinary calculus were not found with US.In all 6 cases urinary calculus were not detected by KUB and/or IVP. Conclusion16-slices CT was a valuable method in diagnosing urinary calculus.

Adult ; Aged ; Aged, 80 and over ; Asbestos ; adverse effects ; Environmental Exposure ; adverse effects ; Female ; Humans ; Male ; Middle Aged ; Pleural Diseases ; chemically induced ; diagnostic imaging ; Tomography, X-Ray Computed