Objective To evaluate the diagnose and operation about the movement cecum syndrome.Methods To analyse clinical manifestations and what finned by operation of the movement cecum syndrom 16 cases,to summarize the issues of diagnose.To use a new operation method-accending colo,cecum shorten treates the movement cecum syndrome,observing the result of the treatment.Results 1 case was used with appendectomy,2 cases was treated with appendectomy and cecum fixxing,clinical manifestation do not disappear.13 cases was treated with appendectomy and accending colo、cecum,clinical manifestation disappear,the weight added.Result of the treament is good.Conclusion appendectomy colo,cecum shorten is a good methed for treating movement cecum syndrom.

0.05). Conclusions The LTs synthesis is elevated in RSV bronchiolitis and LTs may participate in the pathogenesis of RSV bronchiolitis. Leukotriene receptor antagonist may play an important role in RSV bronchiolitis. However there still needs further research on the relationship between LTs and eosinophils count in the pathogenesis of RSV bronchiolitis.

Animals ; Antigens, Neoplasm ; genetics ; metabolism ; Biomarkers, Tumor ; genetics ; metabolism ; Brain Neoplasms ; metabolism ; Cell Adhesion Molecules ; genetics ; metabolism ; Digestive System Neoplasms ; metabolism ; Female ; Genital Neoplasms, Female ; metabolism ; Glioma ; metabolism ; Head and Neck Neoplasms ; metabolism ; Humans ; Immunotherapy ; Male ; Prostatic Neoplasms ; metabolism ; Signal Transduction

Polyamidoamine (PAMAM) dendrimers is considered a better genetic carrier for its ability to form with DNA a nano-sized polyelectrolyte complex of high stability and dissolubility. The mechanism of PAMAMmediated gene endocytosis has been fairly clear and the researches in vitro have confirmed that PAMAM had high transfection efficiency and low cytotoxicity. The researches in vivo have further confirmed that PAMAM had a potential application foreground in the field of gene therapy including in the prevention and treatment of ewing's sarcoma and pulmonary fibrosis, in heart transplantation, and in the preparation of falciparum malaria vaccine.

Objectives To evaluate the application of systemic octreotide radioactive imaging in diagnosis and follow-up for children with neruoblastoma (NB). Methods Clinical data of 14 children with NB applied systemic octreotide radioac-tive imaging from April 2006 to March 2011 were collected and analyzed. For the ifrst-visit patients, they were applied with systemic octreotide radioactive imaging at the ifrst follow-up appointment after routine treatment. And for recurrent or sus-pected recurrent cases, systemic octreotide radioactive imaging was applied before the treatment and after 2-3 weeks chemo-therapy. Results Nine of 14 patients were male and 5 were female with a median age of 4.8 years (1-10 years). According to In-ternational Neuroblastoma Staging System (INSS), 4 cases were categorized as stage 3 and 10 cases were categorized as stage 4. The uptake rate of systemic octreotide radioactive imaging in primary site or metastasis sites of 8 cases were higher than nor-mal site with positive rate of 57.14%(8/14). One case was conifrmed as false positive by treatment and imaging examination. Six cases were negative in primary site or metastasis sites with negative rate of 42.86%(6/14). One case was conifrmed as false negative via treatment and imaging examination. Therefore, the sensitivity of systemic octreotide radioactive imaging reached 85.71%(12/14). Conclusion Systemic octreotide radioactive imaging could be one of the effective methods for NB in diagno-sis, follow-up and evaluation of prognosis.

Objective To establish a method for determination of chlorogenic acid, galuteolin and 3,5-O-dicaffeoyl quinic acid in chrysanthemums by quantitative analysis of multi-components by single marker ( QAMS ) . Methods Using chlorogenic acid as the internal standard substance, the relative correction factors ( RCFs ) of galuteolin and 3, 5-O-dicaffeoyl quinic acid were determined by HPLC.The contents of chlorogenic acid, galuteolin and 3,5-dicaffeoyl quinic acid in 12 samples of chrysanthemums were authentically determined by the external standard method, so as to to verify the accuracy of the QAMS method. Results The average relative error of contents of galuteolin and 3,5-O-dicaffeoyl quinic acid was 2.75% and 1.33%by the external standard method and QAMS, respectively. Conclusion The established RCFs have good reproducibility.In case of lack of standard substances, the QAMS method with chlorogenic acid as an internal standard for determination of 3 indicators in chrysanthemum is feasible.

Objective: To deeply investigate the influential factors of mental health in medical students by structural equation modeling.Methods: 480 medical students were tested with SCL-90,adolescent self-rating life events check list and simplified coping style questionnaire,then theoretical model were established and confirmed for the relationships of life events,satisfaction degree with their profession,coping styles and mental health.Results: Study stress,human relations,satisfaction degree towards their profession and coping styles closely correlated with mental health status,and the structural equation modeling fitted the data very well,GFI and AGFI were both larger than 0.90.Conclusion: Use of structural equation modeling has more advantages than the traditional statistical methods,but it should combine with professional knowledge when it is used.

Thyroid nodules are very common.Its etiological factors include hyperplasia,adenomas,cancer,cysts,and thyroiditis.Most of thyroid nodules are benign.The management of thyroid nodules whether thyroidectomy or observation is according to their nature.So,the key of the therapy is to identify that they are benign or malignant.We should comprehensive evaluate patients' history,physical examination,laboratory examination,and auxiliary examination for judging the nature of thyroid nodules and making decision in the choose of thyroidectomy or observation.

Objective To construct a yeast expression vector containing human TIP60? gene (a spliced form of HIV-1 tat interactive protein,HTATIP,60?103) and screen its interaction proteins by yeast two-hybrid. Methods Human TIP60? gene fragment was amplified by RT-PCR and cloned into pGBKT7 vector. Using TIP60? as bait,the proteins interacting with TIP60? were screened from a human liver cDNA library. The positive clones were analyzed by bioinformatic methods. Results Human TIP60? cDNA was successfully amplified and cloned into the pGBKT7 vector and indentified by double enzyme digestion and DNA sequencing. Using yeast two-hybrid,32 clones were screened from a human liver cDNA library,and 9 positive clones including histone deacetylase 7A (HDAC7A),mouse double minute 2 (DMD2),B-cell CLL/lymphoma 3 (BCL3),endothelin receptor type A (EDNRA),androgen receptor (AR),PHD finger protein 17 (PHF17),Ataxia Telangiectasia Mutated (ATM),CAMP responsive element binding protein 1 (CREB1),orphan steroid receptor BD73 were verified after sequencing. Conclusion The screened interaction proteins with TIP60? by yeast two-hybrid may be involved in the roles of transcriptional regulation of TIP60?.

AIM: To detect the expression of ALEX 1 in the breast cancer tissues in order to verify whether ALEX1 has correlation with clinical pathological features in breast cancer .METHODS:Real-time PCR and immmunohis-tochemistry were applied to detect the expression of ALEX 1 at mRNA and protein levels in the breast tissues .The statistical analysis were performed for determining the correlation with the level of ALEX 1 and the clinical pathological features in breast cancer .RESULTS:The protein levels of ALEX1 in the breast cancer tissues were lower than that in the non-breast cancer tissues (P<0.01).The expression of ALEX1 had correlations with pathological grade , clinical stage, molecular type (P<0.05) but had no correlation with the patients ’ age, tumor size and tumor types in breast cancer .Furthermore, the result of real-time PCR showed that mRNA expression of ALEX 1 was also significantly reduced in the breast cancer tis-sues (P<0.01).CONCLUSION:The expression of ALEX1 in the breast cancer tissues is lower than that in non-breast cancer tissues .The pathological grade and clinical stage in breast cancer are negatively correlated with the expression of ALEX1.