A tolal of 104 rats were at random divided into three groups: the control, light-injured (group 1) and serious-injured (group Ⅱ), with investigating the impact of various traumatic injuries on immunoreactive ?-endophin (ir-?-Ep). The results showed that there was a marked rise for ir-?- Ep in the rats plasma at half an hour of trauma, then reached the peak value at 2h postinjury. The ir-?- Ep concentration in group Ⅱ was significantly higher than group Ⅰ. and the time returned to mormal was 3rd day and 7th day respectively. It is illustrated that both of the increased ir-?-Ep concentration and the time backed to mormal are related to traumatic severity, and therefore, the determination of the changes of ir-?-Ep level in the plasma is beneficial to the judgement of traumatic picture.

AIM:Neural stem cells(NSCs)and their supporting factors are related to nerve regeneration after central nerve injury, but there are few evidences for the changes of NSCs and their derived supporting factors.This study explores the change of NSCs and gene expression of stem cell-derived neural stem/progenitor cell supporting factor(SDNSF)in the injured spinal cord tissues of rats,and investigates the relation between the NSCs and expressions of SDNSF. METHODS:The experiment was carried out in the laboratory of Nantong University Neuroscience Institute between September 2005 and March 2006.①A total of 27 SD rats,cleaning grade,were offered by the Animal Experimental Center of Nantong University.Twelve rats used for immunohistochemistry assay were divided into sham operation group and spinal cord injury group,while other 15 rats used for RT-PCR detection were assigned into sham operation group and spinal cord injury group.All the disposals were in agreement with the ethical standard for animals.②The spinal cord contusion model of rats was established according to Allen's falling strike method.Laminectomy was conducted without strike in the sham operation group.The quantity and morphology of NSCs,gila cells and SDNSF-positive cells were observed by immunochemistry to detect expressions of nestin,glial fibrillary acidic protein(GFAP)and SDNSF in the injured spinal cord on days 8 and 16 after injury.The expression of SDNSF mRNA in the injured spinal cord was studied by semi-quantitative RT-PCR on days 4,8,12,16 after injury. RESULTS:①There were nestin-positive cells about central canal of spinal cord in the sham operation group.The nestin-positive cells showed erupted roots,migrated peripherally and proliferation,some GFAP-positive cells emerged and the SDNSF-positive cells were observed,which were similar to neuron on morphology on day 8 after injury.The quantity of nestin-positive cells decreased obviously,there were a number of GFAP-pesitive cells and few SDNSF-positive cells were showed on day 16 after injury.②RT-PCR revealed that SDNSF mRNA expressed in normal rate,and the expression was up-regulated on day 4 after injury,peaked on day 8,and decreased to the normal level on day 16. CONCLUSION:①There are some NSCs about the central canal of spinal cord,which proliferate and soon may differentiate to gila cell after injury in normal rats.②SDNSF is expressed in the normal spinal cord.The expression of SDNSF mRNA in the spinal cord varies with injured time,accompanying the proliferation and differentiation of NSCs. There are intimate relations between NSCs and expression of SDNSF in the repair of spinal cord injury.

Neurogenic overactivity bladder is a type of overactive bladder with high incidence. This paper reviewed the research progress of neurogenic overactivity bladder in etiology, pathophysiology and treatment.

Objective To observe the clinical effect of point-to-point acupuncture with electric stimulation of direct current induction for facial nerve paralysis. Methods A total of 65 patients with facial nerve paralysis were randomly divided into the observation group (n=33) and the control group (n=32). The control group received routine treatment, and observation group was received Dicang (ST4)-to-Jiache (ST6) and Yangbai (GB14)-to-Taiyang (EX-HN5) by acupuncture with electric stimulation of direct current induction combined with conventional western medicine. The treatment of both groups last 3 weeks. The House-Brackmann Scale and the BR indexe at baseline and after 3 weeks treatment were observed. Results After treatment, The clinical effect of observation group was 66.7%, which was significantly higher than control group 43.8% (U=2.175, P=0.013). After treatment, House-Brackmann facial nerve scale (Ⅰ level=15 cases, Ⅱ level=4 cases, Ⅲ level=3 cases) in the observation group was significantly better than that in the control group (Ⅰ level=5 cases, Ⅱ level=7 cases, Ⅲ level=2 cases; U=2.069, P=0.018). After treatment, R1 latency (12.28 ± 2.55 ms vs. 13.84 ± 3.07 ms, t=2.227) and R2 latency (32.28 ± 7.08 ms vs. 35.95 ± 5.11 ms, t=2.396) in the observation group were significantly shorter than those in the control group (P=0.030, P=0.020). Conclusions The point-to-point acupuncture with electric stimulation of direct current induction could reduce latency of R1 and R2 for facial nerve paralysis, and improve the clinical effect.

Bone Marrow Mesenchymal Stem Cells (BMSCs) are ideal seed cells for cell therapy,which have been through many long-term studies of in vitro and animal disease model treatment and have been widely used in preventing and treating a variety of clinical diseases caused by aging and other damage,including bone injury and regeneration,neural degenerative and injury,myocardial injury,liver cirrhosis,diabetes,lung disease and so on.BMSCs application in the treatment of orthopaedic disease is the most common and effective.By retrospective literature review,we summarize the BMSCs transplantation application in Orthopaedic disease treatment.As an ideal cell source,BMSCs was first applied in bone tissue engineering,mainly used in the treatment of long bone defect and bone nonunion;Recently,the research trends of clinical BMSCs application turned to treat degenerative diseases and genetic disorders because of its great potential,including osteoarthrits,femoral head necrosis,disc degeneration,spinal cord injury,knee varus,osteogenesis hypoplasia,hypophosphatasia and so on.The safety and efficacy of BMSCs treatment are the key issues in clinical cell therapy.There is no side effects and complications after BMSCs treatment,prove its clinical application is safe.But due to type and degree of disease and individual differences,therapeutic methods including injection method,effective concentrations,treatment window selection,cycle and therapeatic effects have a bigger difference.There is still no uniform of recognized operation standards for the treatment of the same type of disease.So BMSCs transplantation requires effective control during whole medical process and its application in orthopedic diseases is still lack of large-scale multicenter clinical study.Therefore,this review focuses on induction,summary and analysis in the research status of BMSCs in bone disease treatment,and provide new ideas and methods for BMSCs transplantation in orthopaedic disease prevention and treatment.

At present implementation of cost accounting is necessary for hospitals to strengthen operational management, rationally allocate medical resources, adapt to competitions on the medical market, perfect the excitation mechanism, and standardize medical management. Cost accounting in Chaoyang Hospital has transformed from an imperfect stage lasting from 1997 to 1999 to a fairly perfect stage dating from 2000. As a result of the implementation of cost accounting, the awareness of costs and effects on the part of the entire staff in the hospital has been enhanced, competition for equipment, space, manpower, etc. among the various departments has been restrained, and the outcome of the hospital has been markedly improved.

Kawasaki disease,also known as multiple mucocutaneous lymph node syndrome,is a self-limiting and acute systemic vasculitis.Kawasaki disease is often complicated by coronary artery lesions.Kawasa-ki disease is a leading cause of coronary artery lesions in childhood in developed countries.The mechanisms of coronary artery lesions and new therapeutic regimens have been the hotpots in research of Kawasaki disease.This paper reviews current research on mechanism of coronary artery lesions and development of new therapeutic regi-mens.

OBJECTIVE:To probe into the status quo and requirements of drug utilization in Haiqin Sanatorium Area of Nanjing Military Region Hangzhou Sanatorium.METHODS:The drug utilization in 710 convalescents in Sanatorium Area from May 2007 to April 2008 was analyzed statistically.RESULTS:A total of 10 categories of drugs were used in the 710 convalescents,chiefly including anti-cold drugs,antihypertensive drugs,drugs for external use,antimicrobial agents,ophthalmic drugs,otorhinolaryngological drugs,vitamins and gastrointestinal drugs.The rate of drug use conforming diagnosis was 87.32%.Oral administration occupied 71.13%.CONCLUSION:The drug utilization in convalescents in Haiqin Sanatorium Area is basically rational.However,there are also some unreasonable points in the drug utilization which remain to be tackled further.

OBJECTIVE:To observe the hypotensive effect of Urapidil in operation on patients with fundus oculi disease accompanying hypertension.METHODS:Urapidil was injected iv first and then dripped.The SP,DP and heart rate were ob?served at given times and ECG was monitored.RESULTS:The total effective rate was95.1%,average SP decreased43.97mmHg(P

Bone marrow stem cells (BMSCs) are a type of self-renewing,multipotential stem cell group that mainly locate in bone marrow.Due to their great expansion,seLf-renewing and the potential ability of differentiating into a wide variety of cell types,BMSCs have become ideal target cells of tissue engineering,cell transplantation and gene therapy.The ophthalmology-related BMSCs research is primarily focused on the therapy of the corneal diseases and retinal diseases.The therapy of the retinal diseases include vitreous cavity injection and subretina transplantation.Both of them get some exciting effectiveness.However,less reports of the application of BMSCs in optic nerve diseases are seen up to now.The research about BMSCs provides a new clue for solving the modern ophthalmology diseases.But the research remains still in an animal experiment stage.Many relevant problems need to solve prior to its going into clinical trial.This review sumumarized the development,characteristics and prospect of BMSCs.