1.Expression of MAGE A 1-6 and SSX 1-9 Genes in the Sputum and Cancer Tissue of the Lung Cancer Patients.
Yeun Jae LEE ; Jang Hoon LEE ; Jung Cheul LEE ; Kwan Ho LEE
Tuberculosis and Respiratory Diseases 2011;70(4):315-322
BACKGROUND: A variety of diagnostic modalities for lung cancer have been developed. To achieve efficient and early detection of lung cancer, we tried to measure the expression rates of the melanoma associated gene (MAGE) and synovial sarcoma on X chromosome (SSX) genes. METHODS: We designed primers for the SSX gene. In addition to the pre-developed MAGE A primer, using an SSX gene primer was attempted to increase the detection rate. We obtained cancer tissues and cancer-free lung tissues from resected lung, sputum from lung cancer patients who had not undergone surgery, and sputum from healthy people and patients with benign intrathoracic diseases. RESULTS: The sensitivity of the MAGE or SSX gene RT-PCR to identifying cancer tissue of the 69 lung cancer patients was 95.2% for squamous cell carcinoma (scc), 87.0% for adenocarcinoma, and 100% for small cell carcinoma. The mean sensitivity value was 94.2% (p=0.001). For adenocarcinoma, the additional use of the SSX gene resulted in a higher expression rate than MAGE alone (87% vs. 69.6%). The expression rate for the cancer-free lung tissue was 14.3% in scc, 17.4% in adenocarcinoma, and 25.0% in small cell carcinoma. In the induced sputum of 49 lung cancer patients who had not undergone surgery, the expression rate for one of the two genes was 65.5%. The expression rate for the sputum of healthy people and benign intrathoracic diseases by MAGE or SSX gene reverse transcription polymerase chain reaction (RT-PCR) was 3.8% and 17.7%. CONCLUSION: Detecting lung cancer using the expression of MAGE and SSX genes in lung cancer tissue has high sensitivity.
Adenocarcinoma
;
Carcinoma, Small Cell
;
Carcinoma, Squamous Cell
;
Humans
;
Lung
;
Lung Neoplasms
;
Melanoma
;
Polymerase Chain Reaction
;
Reverse Transcription
;
Sarcoma, Synovial
;
Sputum
;
X Chromosome
2.Clinical Significance of p53 Gene Expression with Immunohistochemical Staining in Ovarian Cancer.
Jae Wook KIM ; Yeun Hae LEE ; Young Tae KIM ; Chung Pil LEE ; Dong Kyu KIM ; Chan Ho SONG
Korean Journal of Gynecologic Oncology and Colposcopy 1995;6(1):7-12
Mutation of p53 gene is one of t.he commonest genetic changes in the development of human cancer including ovary. We intvestigated the diagnostic usefulness of the demonstration of p53 gene immunoreactivity in ovarian cancers, because immunochemical demonstration of p53 immunoreactivity is inexpensive, easily controlled and can be applied in routine pathology laboratories. p53 immunoreactivity was not identified in any patients in whom there was no morphological evidence of neoplasia. ln contrast, in contrast, in 46% of patients of ovarian cancer, p53 immunoreactivity was identified. Overexpression of gene correlated with advanced stage but did not corre1ate with grade, cell type and tumor size.
Female
;
Genes, p53*
;
Humans
;
Immunohistochemistry
;
Ovarian Neoplasms*
;
Ovary
;
Pathology
3.Customized Orbital Decompression Surgery Combined with Eyelid Surgery or Strabismus Surgery in Mild to Moderate Thyroid-associated Ophthalmopathy.
Seung Woo CHOI ; Jae Yeun LEE ; Helen LEW
Korean Journal of Ophthalmology 2016;30(1):1-9
PURPOSE: To evaluate the efficacy and safety of customized orbital decompression surgery combined with eyelid surgery or strabismus surgery for mild to moderate thyroid-associated ophthalmopathy (TAO). METHODS: Twenty-seven consecutive subjects who were treated surgically for proptosis with disfigurement or diplopia after medical therapy from September 2009 to July 2012 were included in the analysis. Customized orbital decompression surgery with correction of eyelid retraction and extraocular movement disorders was simultaneously performed. The patients had a minimum preoperative period of 3 months of stable range of ocular motility and eyelid position. All patients had inactive TAO and were euthyroid at the time of operation. Preoperative and postoperative examinations, including vision, margin reflex distance, Hertel exophthalmometry, ocular motility, visual fields, Goldmann perimetry, and subject assessment of the procedure, were performed in all patients. Data were analyzed using paired t-test (PASW Statistics ver. 18.0). RESULTS: Forty-nine decompressions were performed on 27 subjects (16 females, 11 males; mean age, 36.6 +/- 11.6 years). Twenty-two patients underwent bilateral operations; five required only unilateral orbital decompression. An average proptosis of 15.6 +/- 2.2 mm (p = 0.00) was achieved, with a mean preoperative Hertel measurement of 17.6 +/- 2.2 mm. Ocular motility was corrected through recession of the extraocular muscle in three cases, and no new-onset diplopia or aggravated diplopia was noted. The binocular single vision field increased in all patients. Eyelid retraction correction surgery was simultaneously performed in the same surgical session in 10 of 49 cases, and strabismus and eyelid retraction surgery were performed in the same surgical session in two cases. Margin reflex distance decreased from a preoperative average of 4.3 +/- 0.8 to 3.8 +/- 0.5 mm postoperatively. CONCLUSIONS: The customized orbital decompression procedure decreased proptosis and improved diplopia, in a range comparable to those achieved through more stepwise techniques, and had favorable cosmetic results when combined with eyelid surgery or strabismus surgery for mild to moderate TAO.
Adolescent
;
Adult
;
Decompression, Surgical/*methods
;
Exophthalmos/*surgery
;
Eye Movements/physiology
;
Eyelids/*surgery
;
Female
;
Graves Ophthalmopathy/*surgery
;
Humans
;
Male
;
Middle Aged
;
Oculomotor Muscles/surgery
;
*Ophthalmologic Surgical Procedures
;
Orbit/*surgery
;
Retrospective Studies
;
Strabismus/*surgery
;
Visual Field Tests
;
Visual Fields/physiology
4.Botulinum Toxin A Treatment for Patients with Periorbital Spasm after Facial Nerve Paresis.
Jae Yeun LEE ; Jong Seo PARK ; Helen LEW
Journal of the Korean Ophthalmological Society 2011;52(8):910-915
PURPOSE: To evaluate clinical features of periorbital spasm and facial asymmetry in the patients who recovered poorly from Bell's palsy and facial trauma and to investigate the effect of Botulinum toxin A as a treatment for periorbital spasm and facial asymmetry. METHODS: Between November 2001 and January 2010, Botulinum toxin injection was performed in 17 patients who had blepharospasm and facial asymmetry following poor recovery from facial palsy. The past history, trauma history, clinical manifestation of blepharospasm, Botulinum toxin A injection dose, injection site, frequency of injection, and duration of effect was evaluated. Data was analyzed using the Mann-Whitney U test, SPSS 12.0. RESULTS: The mean number of injections was 2.7 +/- 2.4 times and the mean dose per injection unit was 12.2 +/- 1.2 units. The Botulinum toxin effect lasted 6.9 +/- 5.5 months in Bell's palsy patients, and 8.0 +/- 4.2 months in trauma patients. There was no significant difference between the 2 groups. Most patients reported improvement of periorbital spasm and facial asymmetry. After treatment, 1 patient complained of epiphora and 1 patient complained of ptosis; conservative treatment was performed for these patients. CONCLUSIONS: Blepharospasm can be treated and a cosmetic improvement in facial symmetry can be achieved by Botulinum toxin A injection in the patients who recover poorly from facial palsy.
Bell Palsy
;
Blepharospasm
;
Botulinum Toxins
;
Cosmetics
;
Facial Asymmetry
;
Facial Nerve
;
Facial Paralysis
;
Humans
;
Lacrimal Apparatus Diseases
;
Paresis
;
Spasm
;
Synkinesis
5.Oral allergy syndrome in pollen - sensitized patients.
You Sook CHO ; Yeun Jeong LIM ; Jae Cheon LEE ; Seoung Ho KIM ; Mi Kyoung LIM ; Bin YOO ; Hee Bom MOON
Journal of Asthma, Allergy and Clinical Immunology 1998;18(3):458-465
BACKGROUND: Oral allergy syndrome(OAS) is composed of it,ching sense and edema in oral cavity, lips, throat, pharynx, and larynx following eating some fresh fruits or vegetables. It has been known that most of patients with OAS are allergic to pollens. Common epitopes were found among pollens, fruits and vegetables. Although OAS is a common farm of food allergy in adults, this is the first epidemiologic study of OAS in Korea. MATERIAL AND METHOD: One hundred and fifty one patients who showed positive skin reaction to pollens were telephone-interviewed. Investigation of the prevalence and clinical manifestations of OAS was possible in 81 patients. RESULT: The prevalence of OAS among these patients was 34.6% (28/81). OAS was found in 24(48%) out of 50 patients sensitized to tree pollens, whereas 4(13%) of 31 grass or weed pollen-sensitized paients had OAS. Most common causative food was apple and all of 17 apple- OAS patients were sensitized to tree pollens. Peach was the second common food and 14 of 15 peach-OAS patients were sensitized to tree pollens. Besides oral symptoms, rhinitis, asthma, diarrhea, nausea, vomiting or generalized urticaria were accompanied in half of the OAS patients (14/28). Some patients showed OAS to some unique Korean foods such as dropwort, taro and Aster. CONCLUSION: OAS was very common in pollen-sensitized patients. Larger epidemiologic studies are needed to find unique Korean foods and their antigensm causing OAS.
Adult
;
Asthma
;
Colocasia
;
Diarrhea
;
Eating
;
Edema
;
Epidemiologic Studies
;
Epitopes
;
Filipendula
;
Food Hypersensitivity
;
Fruit
;
Humans
;
Hypersensitivity*
;
Korea
;
Larynx
;
Lip
;
Mouth
;
Nausea
;
Pharynx
;
Poaceae
;
Pollen*
;
Prevalence
;
Prunus persica
;
Rhinitis
;
Skin
;
Urticaria
;
Vegetables
;
Vomiting
6.A Case of Group O without Anti-A,B due to hypogammaglobuluinemia.
Jae Ho LEE ; Kyung Hae JUNG ; Kyou Sup HAN ; Bok Yeun HAN ; Jong Hyun YOON ; Sa Ail CHUN
Korean Journal of Blood Transfusion 1996;7(2):269-273
An ABO discrepancy was observed in a 19-year-old man clinically diagnosed as congenital hypogammaglobulinemia. The patient's red cell was typed as group O, and his serum had no ABO isoagglutinins. Absence of A antigen and B antigen on patient's RBC was confirmed by adsorption- elution test and saliva test. A-transferase and B-transferase activities were not demonstrated in patient's serum. Patient's serum protein electrophoresis revealed hypogammaglobulinemia pattern, and immunoglobulin levels were markedly decreased. Complete absence of B lymphocytes was observed on patient's lymphocyte subset profile. Patient's father and mother were typed as blood group O and no abnormalities were recognized in their serum protein electrophoresis patterns and immunoglobulin concentration.
Agammaglobulinemia
;
B-Lymphocytes
;
Electrophoresis
;
Fathers
;
Humans
;
Immunoglobulins
;
Lymphocyte Subsets
;
Mothers
;
Saliva
;
Young Adult
7.A Case of Rosai-Dorfman Disease Affecting the Palpebral Conjunctiva.
Jae Yeun LEE ; Hyun Min SHIN ; Helen LEW
Journal of the Korean Ophthalmological Society 2013;54(2):346-350
PURPOSE: To report a single case of Rosai-Dorfman disease of the palpebral conjunctiva with a review of the relevant literature. CASE SUMMARY: A 39-years-old woman presented with a palpebral conjunctival mass 3 weeks in duration on both eyes. The patient had a history of excisional biopsy of lymphadenitis and wanted to remove the mass for cosmetic reasons. An excisional biopsy was performed to obtain a diagnosis for proper management. The histopathologic examination of the lesion showed an intensive proliferation of monotonous and histiocytoid cells beneath the epidermis. Immunohistochemical staining for the S-100 protein was positive and anti-CD1A antibody was negative. Five months after mass excision, there was no evidence of recurrence.
Biopsy
;
Conjunctiva
;
Cosmetics
;
Epidermis
;
Eye
;
Female
;
Histiocytosis, Sinus
;
Humans
;
Lymphadenitis
;
Recurrence
;
S100 Proteins
8.Hydroxyl Radical Production after Intrastriatal Injection of Dopamine and the Effect of Growth Hormone on the Apoptosis of Striatal Neurons Injured by Hypoxia-ischemia in Newborn Rat Brain.
Jae Ju CHO ; Jeesuk YU ; Youn Hee JEE ; Soon Bum LEE ; Soo Yeun OH ; Hyung Gun KIM ; Young Pyo CHANG
Journal of Korean Society of Pediatric Endocrinology 2004;9(2):145-151
PURPOSE:We investigated the production of oxygen hydroxyl radicals in the striatum of neonatal rat brain after intrastriatal injection of dopamine (DA) and the effect of growth hormone (GH) on the apoptosis of striatal neurons injured by hypoxia-ischemia. METHODS:The extracellular striatal levels of 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA as indicators of hydroxyl radical(OH-) production were measured by in vivo microdialysis in the striatums of 7 day-old newborn rats (n=10) after direct intrastriatal infusion of dopamine hydrochloride (1.0 micromol/microL). The samples of perfused artificial cerebrospinal fluid (CSF) were collected every 10 minutes interval. The levels of DA, 2,3-DHBA and 2,5-DHBA of CSF were analysed by HPLC (high performance liquid chromatography). Also, the brains were removed at 24 hour after hypoxic-ischemic injury by Rice-Vannucci method. The coronal sections (12 micrometer) of paraffin-fixed brains were stained by TUNEL (terminal transferase-mediated dUTP nick-end-labelling) technique, and the neuronal cells undergoing apoptosis in the striatum were observed by fluorescent microscopy and compared between GH-treated (50 mg/kg, Dong-Ah Pharmacy Co.) and saline-treated rats. RESULTS:The extracellualr striatal levels of 2,3-DHBA and 2,5-DHBA increased abruptly in the first 10 minutes samples after intrastriatal injection of DA. After then, the levels declined slowely. The levels of striatal extracelluar 2.3-DHBA increased up to 621.8+/-508.7% of basal levels (P<0.05), and the levels of 2.5-DHBA increased up to 262.8+/-198.1% of basal levels (P<0.05). GH reduced markedly the number of apoptotic neuronal cells in the striatum after hypoxic-ischemic brain injury. CONCLUSION: The level of hydroxyl radicals increased abruptly after intrastriatal injection of DA and GH reduced markedly the number of apoptotic neuronal cells in the striatum after hypoxic-ischemic brain injury.
Animals
;
Apoptosis*
;
Brain Injuries
;
Brain*
;
Cerebrospinal Fluid
;
Chromatography, High Pressure Liquid
;
Dopamine*
;
Growth Hormone*
;
Humans
;
Hydroxyl Radical*
;
In Situ Nick-End Labeling
;
Infant, Newborn*
;
Microdialysis
;
Microscopy
;
Neurons*
;
Oxygen
;
Pharmacy
;
Rats*
9.A Case of Tuberculosis of Lid.
Jae Hong AHN ; Chang Yeun LEE ; Ho Min LEW ; Kwang Hwa PARK
Journal of the Korean Ophthalmological Society 1996;37(8):1293-1297
Infection of the lids by the Mycobacterium tuberculosis is uncommon. It usually arises by local extension from the skin adjacent to the lid, but it occasionally arises from the conjunctiva or hematogenous spread. Primary infection of the eyelid by Mycobacterium tuberculosis is very rare. The lid lesion shows brownish red papule that develops into an indurated nodule or plaque; it is occasionally associated with skin ulceration and regional lymphadenopathy. The diagnosis is established through specific histopathologic findings, tuberculin skin testing, and the stain and culture of acid-fast bacilli. It is also done indirectedly through the response to anti-tuberculosis medications. The regimen for treatment of tuberculosis is isoniazid and rifampin taken daily for 6 months. The authors experienced a case of tuberculosis of the lid which occurred in a 4 year-old female whose skin of the upper and lower eyelids showed brownish red nodules and which was treated with anti-tuberculosis medications.
Child, Preschool
;
Conjunctiva
;
Diagnosis
;
Eyelids
;
Female
;
Humans
;
Isoniazid
;
Lymphatic Diseases
;
Mycobacterium tuberculosis
;
Rifampin
;
Skin
;
Skin Tests
;
Skin Ulcer
;
Tuberculin
;
Tuberculosis*
10.Clinical Characteristics of Intermittent Exotropia Patients who Have Improved due to Corrected Refractive Errors.
Jae Yeun LEE ; Sung Woo HA ; Helen LEW
Journal of the Korean Ophthalmological Society 2012;53(6):819-824
PURPOSE: To evaluate clinical features of patients who have experienced decreased exodeviation after refractive error correction. METHODS: Thirty-four patients who experienced decreased exodeviation after refractive error correction between 2003 and 2010 were evaluated. Visual acuity, refractive errors, reported age at onset, duration of wearing glasses, stereopsis, and function of fusion were evaluated. RESULTS: The present study included 34 patients with intermittent exotropia. Before correcting refractive errors, the mean visual acuity was 0.34 +/- 0.27 for the right eye and 0.37 +/- 0.27 for the left eye according to log MAR. After refractive error correction, mean visual acuity improved to 0.12 +/- 0.11 for the right eye and 0.11 +/- 0.11 for the left eye according to log MAR. Before correcting refractive errors, the amount of deviation was 18.1 +/- 7.4 PD at near and 13.5 +/- 8.4 PD at far. However, after correcting refractive errors, the amount of deviation decreased to 5.8 +/- 8.6 PD at near and 4.5 +/- 7.6 PD at far (p < 0.05). CONCLUSIONS: The clinical characteristics of children who have decreased exodeviation after correcting refractive errors is shown by a greater amount of deviation at near than at distance and good fusional vergence and stereoacuity. Because correcting refractive errors by wearing appropriate glasses can decrease exodeviation, the authors of the present study recommend correcting refractive errors as the initial management for exotropia.
Child
;
Depth Perception
;
Exotropia
;
Eye
;
Eyeglasses
;
Glass
;
Humans
;
Myopia
;
Refractive Errors
;
Visual Acuity