Introduction: Worldwide fractures among individuals are a major public health problem. As a result, fracture are the most common cause of severe enduring pain and physical disability, and they affect innumerable globally. It has been studied that nutrients that contain high protein helps in the fracture healing process. Eels contains many nutrients, such as phosphate, calcium, protein, collagen and amino acids that can help bone growth. The aim of this study was to examine the effect of eel flour (Monopterus albus) at multilevel dose content to accelerate the healing process of fracture in male Rattus novergicus Wistar Albino rat. Methods: This study design include experimental study with post-test only on controls group. The wok was conducted from May-June 2019 in the Laboratory of Anatomy of Stikes Piala Sakti Pariaman. The sampling was purposive sampling technique with a sample size of 24 rats. Results: The results of the ANOVA test have proved that administration of eel flour accelerates the healing process of the fracture showing 0.000, followed by Post Hoc LSD to determine the test of significant differences between groups. Conclusion: It was observed that with the increase in the dose of flour given to Wistar Albino rat fracture healing process became faster. From the present study it was evident that consumption of eel flour can accelerate the process of fracture healing. But there is need for further testing on humans as an effort to test the safety of the dose.