Objective To discuss the relationship between monocyte CD36 expression and carotid intima-media thickness (cIMT) in rheumatoid arthritis (RA) patients.Methods A total of 207 RA patients were divided into arteriosclerosis group and non-arteriosclerosis groups in accordance with the results of ultrasound in carotid artery,and semi-quantity score was used for cIMT in patients with RA.Risk factors of cIMT in RA patients were analyzed by Spearman correlation and Logistic regression analysis.Results C reactive protein (CRP),triglyceride (TG),tumor necrosis factor (TNF),interleukin-6 (IL-6) and monocyte CD36 expression were found to be statistically different between the two groups (P ＜0.05).Monocyte CD36 fluorescence intensity was negatively correlated with TNF,IL-6,CRP and cIMT in patients with RA (r=-0.344,P＜0.01;r=-0.646,P＜0.01;r=-0.160,P=0.021;r=-0.687,P＜0.01).Logistic analysis showed that monocyte CD36 was associated with the formation of artery atheromatous plaque in patients with RA [OR=1.580,95％CI (1.057,2.36l),P＜0.01].Receiver operating characteristic curve (ROC) curve analysis showed that monocyte CD36 fluorescence intensity was useful when the value with the area under curve was 0.926,the sensitivity and specificity was 83.7％ and 82.5％ respectively.Conclusion Monocyte CD36 fluorescence intensity is correlated with artery atheromatous plaque in patients with RA,monocyte CD36 fluorescence intensity may be a marker to estimate cIMT in RA patients.

Objective To investigate the distribution of E-selectin polymorphisms in Guangxi ethnic Zhuang population and the association of E-selectin gene polymorphism in exon 2 and in exon 4 with systemic lupus erythematosus(SLE). Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to study the E-selectin polymorphisms in 82 patients with SLE and 95 healthy controls of ethnic Zhuang population in Guangxi Province. Results The frequencies of GG?GT?TT genotypes in the exon 2 of E-selectin were 86.6%,12.2%, and 1.2% in SLE group; 90.5%, 8.4%, and 1.1% in the control group, respectively. The frequencies of AA?AC?CC genotypes in the exon 4 and E-selectin were 81.7%,15.9%, and 2.4% in SLE group; 92.6%,7.4%, and 0.0% in the control group, respectively. There was a significant difference in the frequencies of allele and genotype in E-selectin A561C polymorphism between SLE group and control group(P

Objective To study the distribution of IL-6 gene promoter 572C/G, 634C/G polymorphisms in Han population of Guangxi province, and analyze the relation between IL-6 gene polymorphisms and plasma lipid, platelet count. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to detect IL-6 genotype in 198 healthy Han adults. At the same time the plasma lipid level and platelet count were determined by routine methods. Results Serum lipid levels had not significant difference among the different genotypes of IL-6 (P

BACKGROUND: Polymorphism of exon 5 +3953C/T of interleukin-1β (IL-1β) gene is found in some studies. This gene polymorphism is related to stomach cancer, rheumatism, arthritis and other diseases. However, what is the distribution property of genotype of IL-1β gene in Guangxi Zhuang population? OBJECTIVE: To observe the distribution of the polymorphism of exon 5 +3953C/T of 1L-1β gene in Chinese Guangxi Zhuang population and Han population, and compare its distribution with Caucasians and African white people.DESIGN: Contrast observation.SETTING: Department of Histology and Embryology,Youjiang Medical College for Nationalities.PARTICIPANTS: Persons who received the health examination in the Affiliated Hospital of Youjiang Medical College for Nationalities were recruited in the experiment. Among them, 155 were Zhuang nationality, including 80 male and 75 female, aged (37) years, and 195 were Han na tionality, including 100 male and 95 female, aged (38) years. Blood relationship was not found among them,and informed consents were obtained from all the participants.METHODS: This experiment was carried out in the Experimental Center for Medical Sciences,Guangxi Medical University from June 2003 to May 2005. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the polymorphism of exon 5 +3953C/T of IL-1β gene in Guangxi Zhuang population (n=155) and Han population (n=195).Genotype and allele frequency of IL-1β gene of healthy population were directly calculated with counting method, and they were compared between Caucasians and African white people combining with literatures.MAIN OUTCOME MEASURES: Comparison of genotype frequency and allele taking frequency of IL-1β gene (+3953) among different ethnic populations.RESULTS: Subjects who received healthy examination including 155 Guangxi Zhuang nationality and 195 Chinese Han nationality participated in result analysis. ①The frequencies of genotypes and allele of IL-1β gene (+3953C/T) were close between Guangxi Zhuang population and Han nationality (Frequency of genotype CC,CT TT and allele C and T of Zhuang population was 94.8％,5.2％,0,97.4％,2.6％,respectively,and that of Han population was 93.3％,6.7％,0,96.7％,3.3％,respectively,P＞0.05).②There were significant differences in genotype distribution and allele taking frequency of IL-1β gene between Caucasians,African white people and Guangxi Zhuang population,Han population (Frequency of genotype CC,CT TT and allele C and T of Caucasians was 52.2％,43.9％,3.9％,74.1％,25.9％,respectively,and that of African white people was 60.4％,33.2％,6.4％,76.4％,23.6％, respectively, P＜0.01).CONCLUSION: The distribution of polymorphism of exon 5 +3953C/T of IL-1β gene is close between Guangxi Zhuang population and Han population,but significant difference exists in comparison with Caucasians and African white people.

Objective: To study and explore the distribution characteristics of rs10975521A/T and rs1929992A/G polymorphism of the IL-33 gene in Chinese Guangxi population. To compare the frequency distribution differences of allele and genotype of two sites among different ethnic. Methods:The polymorphism of rs10975521A/T and rs1929992A/G of IL-33 gene in 283 subjects were analyzed with the methods of Single base extension (PCR-SEB) and DNA sequencing,and the distribution frequency and the differences between groups of that were analyzed statisticaly. Results:Three genotypes of AA,AT and TT were found in rs10975521A/T with the frequency distribution of 12. 7%,53. 0% and 34. 3% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population ( P>0. 05 ) . There were significant differences of the allele frequency of rs10975521A/T in the Guangxi population compared with that in the European (P< 0. 05), han Chinese in Bejing (P< 0. 05) and Japanese people (P< 0. 01). Three genotypes of AA,AG and GG were found in rs1929992A/G with the frequency distribution of 23. 9%,53. 7% and 13. 4% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population(P> 0. 05),but the differences of genotype frequency of rs1929992A/G was statistically significant compared with that in the European,han Chinese in Bejing and Japanese people ( P< 0. 05 ) . There were significant differences of the allele frequency between Guangxi population and European (P< 0. 01),han Chinese in Bejing(P< 0. 05). Conclusion: There are different degrees of discrepancy of rs10975521A/T and rs1929992A/G polymorphism of IL-33 gene among different race and region.

Objective To investigate the relationship between genetic polymorphisms of Nogo gene and nasopharyngeal carcinoma in Zhuang ethnic group in Guangxi Province.Methods Nogo gene polymorphisms were analyzed between patients with nasopharyngeal carcinoma and healthy people as a control in Zhuang ethnic group in Guangxi province using PCR-SBE.Their genotype and allele frequency distribution were compared between case and control group.The frequencies of haplotypes were analyzed with SHEsis software between these two sites in different groups.Results There were no differences between the patients and controls in the genotype or allele frequencies of Nogo gene rs 17046518 site (P ＞ 0.05).But the frequency distribution of T allele was significantly different (P =0.003) in the rs12464595 site.The result of haplotypes analysis showed that GA haplotype and CA haplotype were significantly different between cases and control (P =0.045,P =0.002).Conclusion The CG haplotype,CA haplotype and T allele of Nogo gene rs12464595 site increase the risk of nasopharyngeal carcinoma among Zhuang ethnic group in Guangxi.

Objective To investigate the association between peripheral monocyte CD36 expression and rheum-atoid arthritis (RA).Methods A total of 108 patients with RA and 108 healthy individuals were included between 2014 and 2015.Disease Activity Score (DAS)28-erythrocyte sedimentation rate (ESR) and DAS28-C reactive protein (CRP) were used to estimate disease activity in patients with RA,and monocyte CD36 fluorescence intensity was determined by using flow cytometry.Student's t test or Mann-Whitney U test was used to compare the differences between the two groups.The categorical data was compared using x2 test.Correlations between variables were analyzed with Spearman correlation,Logistic regression analysis was used to identify risk factors associated with RA,we used receiver operating characteristic (ROC) curve to estimate the performance of relevant variables for RA.Results Low density lipoprotein cholesterin (LDL-C) [(2.8±0.8) mmol/L,(2.6±0.6) mmol/L,t=-2.046,P=0.042],ESR[25.5(18.00,40.00) mm/1 h,10.00(4.60,10.00) mm/1 h,Z=-12.786,P＜0.01],CRP [15.9 (6.71,25.43) mg/L,2.28 (1.63,4.60) mg/L,Z=-7.980,P＜0.01] and monocyte CD36 expression [(0.44±0.22)×109/L,(081 ±0.26)×109/L,t=1 1.159,P＜0.01] was statistically different between the two groups.Of note,monocyte CD36 fluorescence intensity was negatively correlated with disease activity in patients with moderate and severe disease (r=-0.146,P=0.036;r=-0.259,P=0.007).In stepwise Logistic regression analysis,monocyte CD36 fluorescence intensity was independently associated with RA [OR=0.803,P=0.002,95％CI (0.791,0.820)].ROC curve analysis showed a significant performance with area under curve of 0.855 [95％CI (0.807,0.904);P＜0.01],the sensitivity and specificity were 80.6％ and 73.1％,respectively,the cutoff values was 0.570.Conclusion Monocyte CD36 fluorescence intensity was independently associated with RA in stepwise Logistic regression analysis.A correlation between monocyte CD36 fluorescence intensity and DAS28-CRP,DAS28-ESR was observed in RA patients.

Objective To study the relationship between polymorphisms of E-selectin gene and ischemic stroke (IS) in Zhuang nationality of Guangxi province. Methods The G98T polymorphism in the exon 2 and the S128R polymorphism in the exon 4 of E-selectin gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing among 205 patients with IS of Zhuang nationality in Guangxi and 210 healthy controls. The serum level of E-selectin was determined by enzyme-linked immunosorbent assay (ELISA). Results IS group showed significantly higher serum levels of E-selectin than control group (P0.05). Conclusion E-selectin S128R polymorphism was associated with IS, and R allele may be a genetic risk factor of IS among Guangxi Zhuang natioalities, in whom the E-selectin SR genotype carriers maybe increase the risk of ischemic stroke by enhancing the E-selectin expression.

Objective:To study the frequencies of allele and genotype distribution of miR-146a C>G(rs2910164) and miR-149 T>C( rs2292832) gene, and to analyze the statistical differences between different racial and nationalities.Methods:The Polymerase Chain Reaction-Single Base Extension ( PCR-SBE) technique and DNA sequencing methods were used for the determination of the SNP in miR-146a C>G and miR-149 T>C gene,and compared with the European, African, Japanese and People in Beijing from the Human Genome Project (HapMap).Results:There were no statistical differences of allele and genotype distribution in miR-146a C>G,miR-149 T>C between female and male group (P>0.05).There were significant difference frequencies of allele and genotype distribution of miR-146a C>G and miR-149 T>C gene by compared with the European, African and People in Beijing( P<0.05).Conclusion:There were gene Polymorphisms of miR-146a C>G and miR-149 T>C in Guangxi populations, and there were significant differences by compared with other ethnic populations, which may play an important role in the human inherited disease research.

Objective To investigate the distribution characteristics of interleukin-27 gene rs17855750 G/T,rs40837 A/G polymorphism in Zhuang populations of Guangxi,and to compare the distribution differences of genotype and allele frequencies of interleukin-27 gene rs17855750 G/T,rs40837 A/G polymorphisms among different races.Methods The interleukin-27 gene rs17855750 G/T,rs40837 A/G polymorphisms were detected by SNaPshot SNP genotyping technique on 168 persons in Zhuang populations of Guangxi,frequencies of genotype and allele of interleukin-27 gene rs17855750 G/T,rs40837 A/G polymorphisms were analyzed in Zhuang populations,and was compared with the other four populations (HapMap-HCB,HapMap-JPT,HapMap-YRI,HapMap-TSI) from HapMap database.Results The most common genotype and allele of interleukin-27 gene rs17855750 G/T polymorphysms were TT(70.2％) and G(50.3％) in Zhuang populations of Guangxi,and the most common genotype and allele of interleukin-27 gene rs40837 G/T polymorphysms were AC(35.7 ％) and C(52.1 ％).There were no significant differences in the genotype and allele frequencies of interleukin-27 gene rs17855750 G/T,rs40837 A/G polymorphysms between male and female gender in Zhuang populations of Guangxi(P＞0.05).The frequencies of allele and genotype distribution of IL-27 gene rs17855750 G/T polymorphisms were not significantly different when compared with HapMap-HCB(P＞0.05),but were significantly different when compared with HapMap-JPT,HapMap-TSI and HapMap-YRI(P＜0.01);The frequencies of allele and genotype distribution of intetleukin-27 gene rs40837 A/G polymorphisms were significantly different when compared with HapMap-HCB(P＜ 0.05),and were significantly different when compared with HapMap-JPT,HapMap-YRI and HapMap-TSI(P＜0.01).Conclusion There are significant differences in the frequencies of allele and genotype distribution of interleukin-27 gene rs17855750 G/T,rs40837 A/G between Zhuang populations and other ethnic populations,and this variation may lead to a variety of clinical manifestation and morbidity of some diseases.