No abstract available.
Yersinia enterocolitica* ; Yersinia*

No abstract available.
Animals ; Cattle* ; Yersinia enterocolitica* ; Yersinia*

BACKGROUND: Aseptic technique and cold storage of blood can reduce the incidence of transfusion-associated infections. But, none of these precautions eliminates the potential of drawing contaminated blood from an asymptomatic carrier with psychrophilic organisms such as Yersinia enterocolitica. We evaluated the ability of WBC-reduction filters to prevent the growth of bacteria in packed RBCs that are artificially inoculated with Y. enterocolitica. METHODS: Twenty units of packed RBCs donated from 20 healthy individuals were divided into 4 groups. Group A and B were inoculated with 10 CFU/mL of Y. enterocolitica and group C and D were inoculated with 100 CFU/mL of Y.enterocolitica. After 24 hours of cold storage, group A and C were filtered through WBC-reduction filter (Sepacell R 500A: Asai medical, Japan) and returned them to storage. Group B and D served as unfiltered controls. We collected blood weekly from day 1 to day 35 of storage. Bacterial growths were compared between 4 groups. RESULTS: The prefiltration WBC count was 8,880/ L (SD 1464.2/ L, n=20). After filtration residual WBC count was 210/ L (SD 99.8/ L, n=10). All cases of group B & D (10 & 100 CFU/mL inoculation without filtration) showed growth over 105 CFU/mL after 3 weeks storage. But in filtered groups, only 1/5 (20%) of group C (100 CFU/mL inoculation with filtration) and 4/5 (80%) of group A (10 CFU/mL inoculation with filtration) showed growth over 105 CFU/mL after 3 weeks. CONCLUSIONS: The use of WBC-reduction filter have ability to reduce the risk of transfusion transmitted bacteremia in packed RBCs.
Bacteremia ; Bacteria ; Filtration ; Incidence ; Yersinia enterocolitica* ; Yersinia*

No abstract available.
DNA* ; Plasmids* ; Restriction Mapping* ; Yersinia enterocolitica* ; Yersinia*

Humans ; Yersinia Infections/diagnosis* ; Yersinia enterocolitica

A nested polymerase chain reaction (PCR) was applied to detect and identify pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. We used photochemical postamplification procedure with 8-methoxypsoralen to control carryover contamination. Using the ail and inv gene, the sensitivity and specificity of DNA amplification by nested PCR was considerably improved. The amplified fragment sizes were 298 bp for the ail gene and 295 bp for the inv gene. Amplification was successful when the template was derived from three sources: purified DNA, aliquots of boiled bacterial suspension and aliquots of lysed bacterial suspension. The detection limits were 10 fg of DNA and 2 * 10 colony forming units (CFU) for Y. enterocolitica and 10 fg DNA and 2 CFU for Y. pseudotuberculosis.
DNA ; Limit of Detection ; Methoxsalen ; Polymerase Chain Reaction* ; Sensitivity and Specificity ; Stem Cells ; Yersinia enterocolitica* ; Yersinia pseudotuberculosis* ; Yersinia*

PURPOSE: With increasing frequency, Yersinia enterocolitica is being recognized as an important bacterial cause of acute gastrointestinal infection with abdominal pain. In addition, the association of Y. enterocolitica infections with acute appendicitis has been suggested. This study was undertaken to ascertain whether Y. enterocolitica is a possible infectious agent in acute appendicitis. METHODS: Between December 2007 and April 2008, 162 patients who underwent appendectomy for presumed appendicitis, enrolled in this prospective study. After surgical excision of appendix, a portion of each specimen was cultured for Y. enterocolitica with highly selective media (Cefsulodin-Irgasan-Novobiocin agar). RESULTS: Pathologically, 150 of the patients had appendicitis and 12 patients had normal appendices. Only one of the 150 patients (0.7%) with appendicitis was found to be culture positive for Y. enterocolitica, while it was not detected from normal appendices. CONCLUSION: The authors were unable to implicate Y. enterocolitica as a major pathogen in acute appendicitis within the Seoul area. However, we thought there to be more need for investigation for association of Y. enterocolitica with acute appendicitis over a broader area and season.
Abdominal Pain ; Appendectomy ; Appendicitis ; Appendix ; Humans ; Prospective Studies ; Seasons ; Yersinia ; Yersinia enterocolitica

To investigate incidence of acute gastroenteritis (AGE) caused by Yersinia enterocolitica and Y. pseudotuberculosis (referred to as Y. enterocolitica-pseudotuberculosis complex) in children, seropositive rates to major 8 serotypes of Y. enterocolitica-pseudotuberculosis complex antigens among 467 sera of three groups of subjects (298 acute gastroenteritis; AGE, 108 miscellaneous pediatric diseases; MPD, and 61 healthy medical students; HMS) were investigated by bacterial agglutination test using standardized, heat-killed, phenolized, smooth 0 antigens. In addition, cross-reactions between yersinia agglutinins and Widal agglutinins in patients with AGE caused by Y. enterocolitica-pseudotuberculosis complex were examined in paired serum specimens. Seropositive rates to Y. enterocolitica-pseudotuberculosis complex among three groups, AGE, MPD, and HMS, were 36.5%, 13.8%, and 14.7%, respectively. Of 109 seropositive AGE patients, the most common type-specific agglutinin which showed predominating agglutinin titer to one of 8 serotypes of Y. enterocolitica-pseudotuberculosis complex antigens was PO4b (27.5%), followed by PO2a (23.8%), EO3 (16.5%), PO5a (13.7%), PO5b (9.1%), PO3 (4.5%), EO9 (2.7%), and POlb (1.8%), in orders. The Widal agglutinins cross-reacted to Y. pseudotuberculosis PO4b antigen, but the yersinia agglutinins were differentiated from Widal agglutinins by rising agglutinin titers in paired serum specimens taken one to three weeks apart. In conclusion, acute gastroenteritis caused by Y. pseudotuberculosis in children must be regarded as a fairly common enteric disease in children.
Agglutination Tests ; Agglutinins ; Child* ; Gastroenteritis* ; Humans ; Incidence ; Phenol ; Students, Medical ; Yersinia enterocolitica* ; Yersinia*

China ; epidemiology ; Humans ; Yersinia Infections ; epidemiology ; microbiology ; Yersinia enterocolitica ; classification ; drug effects ; isolation & purification ; pathogenicity

OBJECTIVETo investigate the distribution of Yersinia enterocolitica in Henan province from 2005 to 2011.

METHODSA total of 6700 samples of stool specimen were collected from diarrhea patients and different domestic animals between 2005 and 2011 from Zhengzhou, Suixian and Dengfeng, as well as flies and the daub specimens of raw and cooked meat products. The bacteria were isolated by cold enrichment method, analyzed by the systematic biochemistry to determine the serotypes and bio-types, and tested the virulence genes by PCR method.

RESULTSA total of 216 strains of Yersinia enterocolitica were isolated from 11 kinds of animal hosts and foods, while 29.63% (64/216) of them were from swine. The dominant epidemic serotypes of the Yersinia enterocolitica were O: 5 and O: 8, accounted for 23.2% (50/216) and 20.4% (44/216), respectively; type 1A was the dominant bio-type, accounted for 84.7% (183/216). The dominant serotype and bio-type differed a lot among various hosts.16 pathogenic strains were isolated from swine, followed by diarrhea patients (6 strains) and dogs (6 strains).

CONCLUSIONThe distribution of the host of Yersinia enterocolitica was widespread, while swine was the dominant animal host.

Animals ; Animals, Domestic ; microbiology ; Bacterial Typing Techniques ; China ; epidemiology ; Humans ; Yersinia Infections ; epidemiology ; Yersinia enterocolitica ; isolation & purification