No abstract available.
Yersinia pseudotuberculosis* ; Yersinia*

No abstract available.
Animals* ; Korea* ; Yersinia pseudotuberculosis* ; Yersinia*

No abstract available.
Nephritis, Interstitial* ; Yersinia pseudotuberculosis* ; Yersinia*

No abstract available.
Mesenteric Lymphadenitis* ; Yersinia pseudotuberculosis* ; Yersinia*

No abstract available.
Mesenteric Lymphadenitis* ; Yersinia pseudotuberculosis* ; Yersinia*

A nested polymerase chain reaction (PCR) was applied to detect and identify pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. We used photochemical postamplification procedure with 8-methoxypsoralen to control carryover contamination. Using the ail and inv gene, the sensitivity and specificity of DNA amplification by nested PCR was considerably improved. The amplified fragment sizes were 298 bp for the ail gene and 295 bp for the inv gene. Amplification was successful when the template was derived from three sources: purified DNA, aliquots of boiled bacterial suspension and aliquots of lysed bacterial suspension. The detection limits were 10 fg of DNA and 2 * 10 colony forming units (CFU) for Y. enterocolitica and 10 fg DNA and 2 CFU for Y. pseudotuberculosis.
DNA ; Limit of Detection ; Methoxsalen ; Polymerase Chain Reaction* ; Sensitivity and Specificity ; Stem Cells ; Yersinia enterocolitica* ; Yersinia pseudotuberculosis* ; Yersinia*

Genotype ; Humans ; Yersinia pestis

OBJECTIVETo establish a gene identification method of Yersinia pestis and Yersinia pseudotuberculosis for plague surveillance.

METHODSAccording to the specific genomic sequences of Y. pestis and Y. pseudotuberculosis, i.e. "pestis Island (PeI)" and "pseudotuberculosis Island (PsI)" and the published genomic sequences of 12 strains of Y. pestis and 4 strains of Y. pseudotuberculosis, the specific identification primers of these sequences were designed.

RESULTSA total of 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis and other intestinal bacteria strains were tested with PCR. Of the 5 pairs of Y. pestis identification primers, PeI2 and PeI11 were specific for Y. pestis. Besides Y. pestis, the primers PeI1, PeI3 and PeI12 could detect part of 57 Y. pseudotuberculosis strains. Of the 5 pairs of Y. pseudotuberculosis identification primers, PsI1 could detect all the 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis. PsI7, PsI16, PsI18 and PsI19 were specific for Y. pseudotuberculosis.

CONCLUSIONThe primers PsI1, PeI 2 and PeI11, PsI7, PsI16, PsI18 and PsI19 can be used in the rapid identification of Y. pestis and Y. pseudotuberculosis, which can be also used to explore the circulation of atypical Y. pestis in quiescent plague foci.

Base Sequence ; China ; epidemiology ; DNA Primers ; Genomics ; Humans ; Plague ; diagnosis ; epidemiology ; Polymerase Chain Reaction ; Population Surveillance ; methods ; Yersinia pestis ; genetics ; Yersinia pseudotuberculosis ; genetics

No abstract available.
Erythema Nodosum* ; Erythema* ; Yersinia pseudotuberculosis* ; Yersinia*

Plague cases were reported at Eawy commune, Eah'leo district of Dak lak province in March 1997. Y. pestis was isolated from 1.42% of rat samples. 2.89% of rat serum samples and 20% of human serum samples contained anti-plague antibody. The reproductive season of X.cheopis has been coincided with plague season, peaking in the dry season. The plague and vector development seasons in Eawy have all typical characteristics of plague in the High-Plateau region
Plague ; Yersinia pestis ; Rats ; Humans