China ; epidemiology ; Humans ; Yersinia Infections ; epidemiology ; microbiology ; Yersinia enterocolitica ; classification ; drug effects ; isolation & purification ; pathogenicity

Yersinia pseudotuberculosis is known to cause septicemia, mesenteric lymphadenitis enteritis and erythema nodosum. Most of the infections were found in European countries, but none in Korea ti11 now. For the first time in Korea Y. pseudotuberculosis was isolated form a 51-year-old ma1e with liver cirrhosis. The patient showed chills, abdominal pain and diarrhea followed by a comatose state. The organism was isolated from both blood and peritoneal fluid. The isolation and identification were difficult as the organism grew slowly and many of the characteristics were similar to other enteric bacilli. The isolate was susceptible to all antibiotics tested in vitro, but our chemotherapy with ampicillin and kanamycin did not save the patient's life.
Antibiotics/pharmacology ; Human ; Male ; Middle Age ; Septicemia/microbiology* ; Yersinia/drug effects ; Yersinia/isolation & purification ; Yersinia Infections/microbiology* ; Yersinia pseudotuberculosis Infections/microbiology*

China ; epidemiology ; Humans ; Plague ; diagnosis ; epidemiology ; Yersinia pestis ; isolation & purification

OBJECTIVETo investigate the distribution of Yersinia enterocolitica in Henan province from 2005 to 2011.

METHODSA total of 6700 samples of stool specimen were collected from diarrhea patients and different domestic animals between 2005 and 2011 from Zhengzhou, Suixian and Dengfeng, as well as flies and the daub specimens of raw and cooked meat products. The bacteria were isolated by cold enrichment method, analyzed by the systematic biochemistry to determine the serotypes and bio-types, and tested the virulence genes by PCR method.

RESULTSA total of 216 strains of Yersinia enterocolitica were isolated from 11 kinds of animal hosts and foods, while 29.63% (64/216) of them were from swine. The dominant epidemic serotypes of the Yersinia enterocolitica were O: 5 and O: 8, accounted for 23.2% (50/216) and 20.4% (44/216), respectively; type 1A was the dominant bio-type, accounted for 84.7% (183/216). The dominant serotype and bio-type differed a lot among various hosts.16 pathogenic strains were isolated from swine, followed by diarrhea patients (6 strains) and dogs (6 strains).

CONCLUSIONThe distribution of the host of Yersinia enterocolitica was widespread, while swine was the dominant animal host.

Animals ; Animals, Domestic ; microbiology ; Bacterial Typing Techniques ; China ; epidemiology ; Humans ; Yersinia Infections ; epidemiology ; Yersinia enterocolitica ; isolation & purification

Animals ; Cattle ; Chickens ; Dogs ; Sheep ; Swine ; Yersinia enterocolitica ; classification ; isolation & purification

OBJECTIVETo investigate the epidemiological and molecular typing features of the pathogenic Yersinia enterocolitica strains isolated in China,using pulsed field gel electrophoresis(PFGE) and standardized PFGE method as well as typing database of Yersinia enterocolitica.

METHODSPFGE analysis was performed as Laboratory Directions for molecular subtyping of Salmonella by PFGE (PulseNet,USA) with some modifications and the results of PFGE were analyzed by BioNumerics soft (Version 4.0, Applied Maths BVBA, Belium).

RESULTS114 O:3 Yersinia enterocolitica strains were typed by 25 patterns to have found that K6GN11C30012 (50 strains), K6GN11C30015(19 strains) and K6GN11C30016(10 strains) were the major patterns. K6GNllC30012 had 92.2% cluster similarity with K6GN11C30009-K6GN11C30023. This clone included 91.23% strains of 114 0:3 Yersinia enterocolitica strains. 51 0:9 Yersinia enterocolitica strains were typed by 14 patterns; K6GN11C90004 (22 strains) and K6GN11C90010 (13 strains)were the major patterns. K6GN11C90004 had 81.8% cluster similarity with K6GN11C90010 patterns. The major patterns of 0:3 and 0:9 serotypes were quite different.

CONCLUSIONO:3 Yersinia enterocolitica strains might originate from the same clone and had very few variation in different years and provinces but O:9 Yersinia enterocolitica strains from two different clones with some changes.

China ; Electrophoresis, Gel, Pulsed-Field ; Humans ; Yersinia enterocolitica ; classification ; genetics ; isolation & purification

OBJECTIVETo study the epidemiology of genotyping Yersinia pestis isolated in the fulminant epidemics of human plague in Qinghai province in 2004.

METHODSPrimer pairs targeting the twenty-three different identified regions (DFRs) were designed to detect the presence or deletion of each DFR in 13 strains of Yersinia pestis isolated from the fulminant epidemic of human plague in Qinghai province in 2004.

RESULTSThere were 4 genomovars, i.e. Genomovar 8, 10, 15 and 16 in the 13 strains of Yersinia pestis identified. The genomovar of all the strains of Yersinia pestis isolated from Nangqian county was Genomovar 10. Among the two strains of Yersinia pestis isolated from Wulan county, the genomovar of one strain was Genomovar 8 and the other was Genomovar 10. The genomovars of all the strains of Yersinia pestis isolated from Qilian, Qumalai and Chengduo county belonged to Genomovar 16.

CONCLUSIONIt was demonstrated that the genotyping of Yersinia pestis appeared to be a powerful tool for investigating human plague epidemics.

China ; epidemiology ; Disease Outbreaks ; Genotype ; Humans ; Molecular Epidemiology ; Plague ; epidemiology ; Yersinia pestis ; genetics ; isolation & purification

BACKGROUND: This study was designed as a quasi-experiment to evaluate automatic inoculation of fecal specimens, using the automated specimen inoculator Previ Isola (bioMerieux, France). METHODS: We evaluated the quality of cultures, recovery rates of enteropathogenic bacteria (Salmonella, Shigella, Campylobacter, and Yersinia species), and cost-effectiveness in terms of technical time. The Previ Isola recovery rates for the two-year period from August 2009 to July 2011 were compared with historical manual inoculation data of the previous two years (August 2007 to July 2009). The regional (Baden-Wurttemberg) and nationwide (Germany) trends of recovery rates for this four-year period were referred. RESULTS: A total of 5,884 fecal specimens were collected over the study period. Most positive cultures were for Salmonella, followed by Campylobacter. Compared with the historical data, the numbers of Campylobacter-positive specimens for a year between August and July were increased significantly, from 19 in 2007-2008 and 10 in 2008-2009 to 32 in 2009-2010 (P=0.002) and 32 in 2010-2011 (P=0.003), respectively. During the study period, the official data for our region and nationwide did not show this increase in the recovery rate of Campylobacter. For Salmonella, Shigella, and Yersinia, no significant changes were observed. Compared with manual inoculation, the mean hands-on time with Previ Isola inoculation was significantly shortened, from 37:30 min to 8:42 min per 15 fecal specimens. CONCLUSIONS: Inoculation by Previ Isola improves the quality of routine culture of fecal specimens, with better sensitivity for Campylobacter and less hands-on time.
Automation ; Bacteria/*isolation & purification ; Bacteriological Techniques/*methods/standards ; Campylobacter/isolation & purification ; Feces/*microbiology ; Humans ; Quality Control ; Salmonella/isolation & purification ; Shigella/isolation & purification ; Yersinia/isolation & purification

OBJECTIVETo analyze the genetic evolution and bacterial type changes of Yersinia enterocolitica in the Ningxia area between year 1984 and 2011.

METHODSA total of 296 strains of Yersinia enterocolitica was collected from diarrhea patients, pig, rodents, sheep and dogs between year 1984 and 2011. The serotype, biotype, ail, ystA, ystB, yadA, virF and other toxic genes were detected. The PFGE subtypes of serotype O:3 and O:9 strains and the cluster features were analyzed.

RESULTSOut of 296 Yersinia enterocolitica strains, pig was the main host, accounting for 65.20% (193/296), followed by rodents, accounting for 32.43% (96/296). Serotype and biotype had their own respective dominant types in different periods. During 1984 and 1985, 2 strains of serotype O:3 and 3 strains of serotype O:9 were isolated, all belonged to biotype 3. Because of lack of strains, there were no obvious dominant types found. Between 1997 and 1999, 177 strains of serotype O:9 Yersinia enterocolitica were isolated as the dominant strain; and there were 178 strains of biotype 2 Yersinia enterocolitica were found. During 2007 and 2011, 54 strains of serotype O:3 Yersinia enterocolitica were isolated as dominant strain; followed by 26 strains of serotype O:5. There were separately 44 and 59 strains of biotype 1A and biotype 3. The PCR test divided the 248 strains into 4 types, including pathogenic strains as type I (ail(+), ystA(+), ystB(-), yadA(+), virF(+)). The PFGE divided the serotype O:3 into 12 types, in which K6GN11C30021 and K6GN11C30012 were the dominant types, accounting for 63.64% (42/66). The serotype O:9 were divided into 14 types, in which K6GN11C90010, K6GN11C90008, K6GN11C30018 and K6GN11C90003 were the dominant types, accounting for 89.01% (162/182).

CONCLUSIONThe different serotypes of isolated strains in Ningxia district showed different dominant bacteria in different periods; while the biotypes also changed with serotypes. The Yersinia enterocolitica isolated from different years showed great variation.

Animals ; DNA, Bacterial ; genetics ; Dogs ; Electrophoresis, Gel, Pulsed-Field ; Genes, Bacterial ; Genetic Variation ; Humans ; Rodentia ; Sheep ; Swine ; Yersinia Infections ; microbiology ; Yersinia enterocolitica ; classification ; genetics ; isolation & purification

OBJECTIVETo establish a highly efficient culture method for detecting Yersinia enterocolitica in stool samples from diarrheic patients.

METHODSStool samples collected from 200 diarrheic patients were detected with a modified and 3 conventional methods, and the positivity rates of the bacterium were compared statistically.

RESULTSWith the modified method, 18 positive samples for Yersinia enterocolitica were detected from 200 stool samples, while with the 3 conventional methods, only 3, 5, and 8 positive samples were identified, respectively. Statistical analysis with McNemer test suggested significant difference in the positive detection rate between the modified and the 3 conventional methods.

CONCLUSIONThe modified method for Yersinia enterocolitica detection has much higher sensitivity than the 3 conventional methods, and can be applied in clinical detection.

Bacteriological Techniques ; Culture Techniques ; methods ; Diarrhea ; microbiology ; Feces ; microbiology ; Humans ; Temperature ; Yersinia enterocolitica ; growth & development ; isolation & purification