Purpose: This study aimed to examine the effects of knowledge of Human Papilloma Virus (HPV) and health beliefs related to HPV vaccination on the intention of cervical cancer prevention behavior in Nurses. Methods: Data were collected by conducting a survey of 222 nurses at a single tertiary hospital in J city from September 26 to October 02, 2019. The data were analyzed in SPSS WIN version 23.0 using descriptive statistics, t-test, ANOVA, Pearson’s correlation coefficient and Multiple linear regression. Results: The mean score for HPV knowledge was 7.98. Regarding health beliefs about HPV vaccination, the mean score for the level of perceived benefit was 3.08, and for perceived sensitivity was 2.09. The factors influencing the intention of cervical cancer prevention behavior were perceived benefits, severity, contraception, clinical career, and experience with HPV testing. Conclusion The results of the study indicate that it is necessary to develop and apply education programs that enhance cervical cancer and HPV knowledge. Health beliefs related to HPV vaccination should be integrated when developing nursing intervention to improve the intention of cervical cancer prevention behavior in nurses.