Allelic deletions involving the short arm of chromosome 3(3p13-21.1) have been observed frequently in cervical carcinomas. Recently the fragile histidine triad(FHIT) gene was cloned and mapped to this chromosomal region(3p14.2). From various studies involving tumor cell lines and primary cancers, the FHIT gene has been presumed to be a candidate for tumor suppressor gene involving various tumors. In FHIT gene, the most common aphidicolin-inducible fragile site, FRA3B exists and the FRA3B has been considered as a region of the spontaneous integration site of HPV 16. In order to elucidate the role of the FHIT in carcinogenesis of cervical cancer, this study was designed to investigate both the expression of FHIT protein in normal, preinvasive and invasive cancer samples employing immunohistochemical study and allelic loss of FHIT gene locus against several microsatellite markers employing the PCR analysis. Immunohistochemical studies of FHIT protein revealed following features. In normal ectocervical squamous epithelium, the expression of FHIT was relatively weak and confined to the basal layer, but in normal endocervical glandular epithelium it was very strong. The expression of FHIT was reduced as the tumor progressed from early lesion to invasive cancer. The koilocytosis was associated with diminished expression of FHIT protein. The study of allelic loss of FHIT gene locus was undertaken against two intragenic (D3S1300, D3S1234) and one extragenic (D3S1295) microsatellite markers. The 5th intron, D3S1300, showed allelic change in 6 of 15 assays and 7th intron, D3S1234 showed allelic change in 10 of 29 assays. There was no apparent LOH from 29 assays in D3S1295. In conclusion, the expression of FHIT protein was markedly reduced or absent in cervical squamous cell carcinoma and the chromosome breakage in FHIT region might be related to the diminished expression of FHIT. On the basis of the reduced expression of FHIT and its encompassment of FRA3B region, it is suggested that disruption of FHIT, a putative tumor suppressor gene, might be the mechanism by which HPV infection enhances cervical tumorigenesis and clonal outgrowth.
Arm ; Carcinogenesis ; Carcinoma, Squamous Cell ; Cell Line, Tumor ; Chromosome Breakage ; Clone Cells ; Epithelium ; Genes, Tumor Suppressor ; Histidine ; Human papillomavirus 16 ; Introns ; Loss of Heterozygosity ; Microsatellite Repeats ; Polymerase Chain Reaction ; Uterine Cervical Neoplasms

No abstract available.
Education*

No abstract available.
Weight Gain*

No abstract available.
Adenoma*

No abstract available.
Aged* ; Humans

No abstract availalbe.
Hepatitis C, Chronic* ; Hepatitis, Chronic* ; Humans

Hypertensive patients tend to experience cardiac diseases such as myocardial infarction more frequently than the normotensive subjects. Exercise training in hypertensive patients alliviates their symptoms, improves their cardiopulmonary endurance, and decreases their cholesterol levels. Regular exercise in hypertensive patients decreases the development of their symptoms, therefore makes patients comfortable due to the decreased sympathetic tones and increased parasympathetic tones. To prescribe exercise to hypertensive patients, it is essential to evaluate the patients' blood pressure while exercising. The components of exercise prescription include the mode, intensity, duration, and frequency of exercise, The best exercise for hypertensive patients is aerobic exercise which improves cardiopulmonary functions. It makes the patients take less medications. The aerobic exercise includes cycling, walking, and running. The intensity of the aerobic exercise should be 45~60% of their VO2max value. While the patient is taking medication, the prescription of exercise should be cautious, because most antihypertensive agents decrease exercise endurance, and thus make it difficult to continue exercise, compared with those without medication.
Antihypertensive Agents ; Blood Pressure ; Cholesterol ; Exercise ; Exercise Therapy* ; Heart Diseases ; Humans ; Hypertension ; Myocardial Infarction ; Prescriptions ; Running ; Walking

An in other medical fields in Korea, the terminology in pathology has to go through a long adaptation period since the liberation from Japan in 1945. The Korean Society of Pathologists (KSP) has been the driving force in establishing and adopting new pathology terms in Korean. This effort started from the time when the KSP launched the Korean Journal of Pathology in 1965, and was activated by the momentum of publishing its pathology textbook for the medical students first in Korea in 1990. The KSP organized the Terminology Standardization Committee in close collaboration with the Termonology Standardization Committee of the Korean Medical Association (KMA). The product of joint works was the third edition of Korean Medical Termonology Dictionary published by the KMA in 1992. Since then, the Termonology Standardization Committee of the KSP has continued to improve many inappropriate terms into more easily understandable ones based on the guideline. The guideline of the KSP is basically same to that proposed and adopted by the KMA. Pathology terms are expected to improve further to be standardized as the Pathology Textbook Committee of the KSP is in process of publishing the 5th edition. In the future, the KSP should be ready to establish and standardize the medical terminology in the pathology field since so many new vocabularies are coming into use in this rapidly changing medical environment.
Cooperative Behavior ; Humans ; Japan ; Joints ; Korea ; Pathology* ; Students, Medical ; Vocabulary

Recent studies have shown that opiods can produce potent antinociceptive effects by interacting with opioid receptors in peripheral tissues. This study sougt to compare the effects of morphine with those of-bupivacaine administered intraarticularly upon pain after arthroscopic knee surgery. In a ramdomized manner, 60 healthy patients received either morphine(3 mg in 20 ml NaCl : n=20), bupivacaine(20 ml, 0.25% : n=20) intraarticularly at the completion of surgery, and others were not administered (n = 20) under general anesthesia after 1, 2, 4, 6, 12 and 24h of postoperative day, pain was assessed by a visual analogue pain scalps, time to first analgesic use were recorded. Pain scores were signicantly greater in the morphine group than two groups at 11. From 4th until the end of the study period, pain scores were significantly greater in the bupivaca.ne group than in the other two group. Anagesic requirements were significantly greater in the morphine group than two groups at 1h but were significantly greater in the bupivacaine group than in the other groups throughout the remainder of the study period. The results suggest that intraarticular morphine produces an analgesic effect of delayed onset but of remarkably long duration.
Anesthesia, General ; Bupivacaine* ; Humans ; Knee* ; Morphine* ; Receptors, Opioid ; Scalp

No abstract available.
Animals ; Carrier Proteins* ; Chemokine CCL5* ; Macrophages* ; Mice*