No abstract available.
Education*

Allelic deletions involving the short arm of chromosome 3(3p13-21.1) have been observed frequently in cervical carcinomas. Recently the fragile histidine triad(FHIT) gene was cloned and mapped to this chromosomal region(3p14.2). From various studies involving tumor cell lines and primary cancers, the FHIT gene has been presumed to be a candidate for tumor suppressor gene involving various tumors. In FHIT gene, the most common aphidicolin-inducible fragile site, FRA3B exists and the FRA3B has been considered as a region of the spontaneous integration site of HPV 16. In order to elucidate the role of the FHIT in carcinogenesis of cervical cancer, this study was designed to investigate both the expression of FHIT protein in normal, preinvasive and invasive cancer samples employing immunohistochemical study and allelic loss of FHIT gene locus against several microsatellite markers employing the PCR analysis. Immunohistochemical studies of FHIT protein revealed following features. In normal ectocervical squamous epithelium, the expression of FHIT was relatively weak and confined to the basal layer, but in normal endocervical glandular epithelium it was very strong. The expression of FHIT was reduced as the tumor progressed from early lesion to invasive cancer. The koilocytosis was associated with diminished expression of FHIT protein. The study of allelic loss of FHIT gene locus was undertaken against two intragenic (D3S1300, D3S1234) and one extragenic (D3S1295) microsatellite markers. The 5th intron, D3S1300, showed allelic change in 6 of 15 assays and 7th intron, D3S1234 showed allelic change in 10 of 29 assays. There was no apparent LOH from 29 assays in D3S1295. In conclusion, the expression of FHIT protein was markedly reduced or absent in cervical squamous cell carcinoma and the chromosome breakage in FHIT region might be related to the diminished expression of FHIT. On the basis of the reduced expression of FHIT and its encompassment of FRA3B region, it is suggested that disruption of FHIT, a putative tumor suppressor gene, might be the mechanism by which HPV infection enhances cervical tumorigenesis and clonal outgrowth.
Arm ; Carcinogenesis ; Carcinoma, Squamous Cell ; Cell Line, Tumor ; Chromosome Breakage ; Clone Cells ; Epithelium ; Genes, Tumor Suppressor ; Histidine ; Human papillomavirus 16 ; Introns ; Loss of Heterozygosity ; Microsatellite Repeats ; Polymerase Chain Reaction ; Uterine Cervical Neoplasms

No abstract available.
Adenoma*

No abstract available.

No abstract available.
Fatty Liver*

No abstract available.
Aged* ; Humans

No abstract available.
Weight Gain*

No abstract availalbe.
Hepatitis C, Chronic* ; Hepatitis, Chronic* ; Humans

Hypertensive patients tend to experience cardiac diseases such as myocardial infarction more frequently than the normotensive subjects. Exercise training in hypertensive patients alliviates their symptoms, improves their cardiopulmonary endurance, and decreases their cholesterol levels. Regular exercise in hypertensive patients decreases the development of their symptoms, therefore makes patients comfortable due to the decreased sympathetic tones and increased parasympathetic tones. To prescribe exercise to hypertensive patients, it is essential to evaluate the patients' blood pressure while exercising. The components of exercise prescription include the mode, intensity, duration, and frequency of exercise, The best exercise for hypertensive patients is aerobic exercise which improves cardiopulmonary functions. It makes the patients take less medications. The aerobic exercise includes cycling, walking, and running. The intensity of the aerobic exercise should be 45~60% of their VO2max value. While the patient is taking medication, the prescription of exercise should be cautious, because most antihypertensive agents decrease exercise endurance, and thus make it difficult to continue exercise, compared with those without medication.
Antihypertensive Agents ; Blood Pressure ; Cholesterol ; Exercise ; Exercise Therapy* ; Heart Diseases ; Humans ; Hypertension ; Myocardial Infarction ; Prescriptions ; Running ; Walking

No abstract available.