Treatment options for patients with chronic kidney disease (CKD) are currently limited; therefore, there has been significant interest in applying mesenchymal stem/stromal cell (MSC)-based therapy to treat CKD. However, MSCs harvested from CKD patients tend to show diminished viability and proliferation due to sustained exposure to uremic toxins in the CKD environment, which limits their utility for cell therapy. The application of melatonin has been demonstrated to improve the therapeutic efficacy of MSCs derived from and engrafted to tissues in patients suffering from CKD, although the underlying biological mechanism has not been elucidated. In this study, we observed overexpression of hexokinase-2 (HK2) in serum samples of CKD patients and MSCs harvested from an adenine-fed CKD mouse model (CKD-mMSCs). HK2 upregulation led to increased production levels of methylglyoxal (MG), a toxic metabolic intermediate of abnormal glycolytic processes. The overabundance of HK2 and MG was associated with impaired mitochondrial function and low cell proliferation in CKD-mMSCs. Melatonin treatment inhibited the increases in HK2 and MG levels, and further improved mitochondrial function, glycolytic metabolism, and cell proliferation. Our findings suggest that identifying and characterizing metabolic regulators such as HK2 in CKD may improve the efficacy of MSCs for treating CKD and other kidney disorders.

PURPOSE: Unpredictable shrinkage of zirconia during sintering process causes discrepancy. Therefore, there have been attempts to reduce discrepancy by milling zirconia after sintering. However, due to the hardness of sintered zirconia, milling takes longer time, causes damage to the machine and causes chip formation. With customized zirconia block using the mean dimension of prepared natural dentition, it is expected to overcome these shortcomings. MATERIALS AND METHODS: The mean dimension of prepared natural dentition was analyzed as STL file after scanning of prepared teeth treated at SNUDH. The transverse, frontal and sagittal planes were set using Mimics and Photoshop. 3D volume was projected on each plane, and the outer line was measured through external tangent line, and the inner line was measured through inflection point of tangent line. RESULTS: The mean height of prepared incisal (N = 57) is 6.60 ± 1.05 mm, mesiodistal length is 2.98 ± 0.73 mm, buccolingual length is 2.04 ± 0.73 mm. The mean height of prepared premolar (N = 15) is 5.37 ± 1.49 mm, mesiodistal length is 4.10 ± 1.78 mm, buccolingual length is 5.86 ± 1.55 mm. And the mean height of prepared molar (N = 13) is 5.11 ± 1.29 mm, mesiodistal length is 6.80 ± 1.18 mm, buccolingual length is 7.34 ± 1.40 mm. CONCLUSION: Using the mean dimension of prepared natural dentition, it is expected to be able to fabricate customized zirconia block.
Bicuspid ; Dentition ; Hardness ; Molar ; Tooth*

People with class I intellectual disability need lifelong assistance and protection from their surroundings due to impaired adaptive functioning. They have poor oral health and show higher prevalence of dental caries, periapical inflammation and tooth loss that require proper prosthetic restoration. Because removable prostheses for intellectually disabled patients often lack stability, retention, and maintenance, fixed prostheses are essential and the only available option is dental implants. In this case, a 45 year-old male patient with class I intellectual disability had poor oral hygiene with most of his teeth missing and visited the clinic to recover his masticatory function. Due to such systemic conditions, the definitive restoration of choice was the implant-supported fixed dental prosthesis made of biocompatible and highly strong monolithic zirconia. In consequence of the treatment process, the patient was able to improve his oral environment aesthetically and functionally.
Dental Caries ; Dental Implants ; Dental Prosthesis ; Humans ; Inflammation ; Intellectual Disability* ; Male ; Mouth Rehabilitation* ; Mouth* ; Oral Health ; Oral Hygiene ; Prevalence ; Prostheses and Implants ; Tooth ; Tooth Loss

PURPOSE: Human amniotic membrane has been used to variable ocular surface disorder. We report a case of the usefulness of amniotic membrane transplantation (AMT) for vernal keratoconjunctivitis "Shield ulcer". METHODS: A 12-year-old male patient was diagnosed to have vernal keratoconjunctivitis "Shield ulcer" and treated with medical therapy for four weeks. But he showed severe itiching, foreign body sensation, decreased visual acuity and continued to corneoconjunctival lesion and then, Amniotic membrane transplantation with stromal side down was performed after debridement of the ulcer bed and therapeutic contact lens was applied. RESULTS: At tenth day after AMT, ulcer size was decreased and corneal state was generally improved with restoration of visual acuity. One month after, he has been comfortable with improved visual acuity, giant papillae of upper tarsal conjunctiva was nearly complete remission. CONCLUSIONS: AMT is an effective method for managing vernal keratoconjunctivitis "Shield ulcer", that are refractory to conventional medical treatment.
Amnion* ; Child ; Conjunctiva ; Conjunctivitis, Allergic* ; Debridement ; Foreign Bodies ; Humans ; Male ; Sensation ; Ulcer* ; Visual Acuity

An accurate implant placement with ideal location is significant for long-term success of the implant. An exact evaluation of nearby anatomic structures such as quality of residual bone, an inferior alveolar bone and a maxillary sinus is required. For a prosthetic-driven treatment, planned surgery, precise prosthesis and communication with the patient are significant requisites especially for full-mouth rehabilitation. In this case, the patient with severe alveolar bone resorption had a CT guided surgery supported by CT data and the data from scanning diagnostic wax-up. Afterward, edentulous area was restored by full mouth implant-supported prosthesis by using monolithic zirconia and CAD/CAM technique. This paper reports the outcome of the procedure which was remarkable both esthetically and functionally.
Bone Resorption ; Humans ; Maxillary Sinus ; Mouth Rehabilitation ; Mouth ; Prostheses and Implants ; Rehabilitation

PURPOSE: The purpose of this study was to investigate the effects of the insertion depth of an immediately loaded implant on the stress distribution of the surrounding bone and the micromovement of the implant using the three-dimensional finite element analysis. MATERIALS AND METHODS: A total of five bone models were constructed such that the implant platform was positioned at the levels of 0.00 mm, 0.25 mm, 0.50 mm, 0.75 mm, and 1.00 mm depth from the crest of the cortical bone. A frictional coefficient of 0.3 and the insertion torque of 35 Ncm were simulated on the interface between the implant and surrounding bone. A static load of 178 N was applied to the provisional prosthesis with a vertical load in the axial direction and an oblique load at 30°with respect to the central axis of the implant, then a finite element analysis was performed. RESULTS: The implant insertion depth significantly affected the stress distribution on the surrounding bone. The largest micromovement value of the implant was 39.34 µm. The oblique load contributed significantly to the stress distribution and micromovement in comparison to the vertical load. CONCLUSION: Increasing the implant insertion depth was advantageous in dispersing the concentrated stress in the cortical bone and did not significantly affect the micromovement associated with early osseointegration failure.
Dental Implants ; Finite Element Analysis* ; Friction ; Immediate Dental Implant Loading ; Maxilla* ; Osseointegration ; Prostheses and Implants ; Torque

Computer aided design and manufacturing and implant surgery using a guide template improve restoration-driven implant treatment procedures. This case utilized those digital technologies to make definitive prostheses for a patient. According to the work flow of digital dentistry, cone beam computed tomography established the treatment plan, which was followed to make the guide template for implant placement. The template guided the implants to be installed as planned. The customized abutments and surveyed fixed restorations were digitally designed and made. The metal framework of the removable partial denture was cast from resin pattern using an additive manufacturing technique, and the artificial resin teeth were replaced with the zirconia onlays for occlusal stability. These full mouth rehabilitation procedures provided functionally and aesthetically satisfactory results for the patient.
Computer-Aided Design ; Cone-Beam Computed Tomography ; Dentistry ; Denture, Partial, Removable ; Humans ; Inlays ; Mouth Rehabilitation* ; Mouth* ; Prostheses and Implants ; Tooth ; Workflow

PURPOSE: The purpose of this study was to explore the parenting image of modern Korean society through modern novels published during in the stage of modern change in Korea. METHOD: The data were analyzed through latent content analysis. Modern Korean novels (N=138) written during the Korean modernization stage were chosen for analysis. Five categories and seventeen meaningful sub-categories were drawn out from 636 significant sentences. RESULTS: The parenting images expressed in modern Korean society were as follows: From the category of [Devotional love], 5 sub-categories were drawn: Hedgehog's love, Lavish love, Sacrificial care, Exertion of the mind, and Prayer for the future of their children. From the category of [Stern father and affectionate mother], 3 sub-categories were drawn: Stern and Strict father, Tender and loving mother, and Strong maternal love. From the category of [Enthusiasm for their children's education], 3 sub-categories were drawn: Sense of duty to provide good education for their children, Zeal for their children's education, and Satisfaction with their hard-working children. From the category of [The head of family], 3 sub-categories were drawn: Person who has the right to make decisions, Reliable protector, and Object of filial devotion. From the category of [sexual discrimination], 3 sub-categories were drawn: Preference and favoritism to sons, Attaching importance to education of sons, Regarding daughters as those who help support the family. CONCLUSIONS: The results of this study will help to improve the basic understanding the parenting image and parent-child relationship in present day Korea.
Child ; Education ; Fathers ; Head ; Humans ; Korea ; Love ; Mothers ; Nuclear Family ; Parent-Child Relations ; Parenting* ; Parents* ; Religion ; Social Change ; Child Health

PURPOSE: To evaluate the mechanism of the humeral fractures induced by arm wrestling and the clinical results of its treatment. MATERIALS AND METHOD: We reviewed 7 humeral fractures induced by arm wrestling; 3 humeral shaft fractures, 4 humeral medial epicondyle fractures. The mechanism of the fractures and the clinical results were assessed by history and radiographs. RESULTS: Shaft fractures were produced by twist and axial compression force. Humeral medial epicondyle fractures were the avulsion fractures by excessive contraction of flexor muscles and developed in young age. We operated 6 of them and in all cases, we could obtain fracture healings without complication. CONCLUSION: The humeral fractures induced by arm wrestling have the differences in the ages and mechanisms as to the locations of the fractures and if the proper treatment is performed, the clinical results are satisfactory.
Arm* ; Fracture Healing ; Humeral Fractures ; Humerus* ; Muscles ; Wrestling*

PURPOSE: To analyse the efficacy of MRI in assessing fracture configuration of tibial plateau. MATERIALS AND METHODS: The subjects were consisted of the MR images and the plain radiographs of the thirty one patients with tibial plateau fracture. With the plain radiographs, the fractures were classified according to the Schatzker's system and the amount of depression and displacement, location of the major fragment were measured. Then the same parameters were measured with MR images. Additionally new fracture lines and resulting fragments which can be fixed with a screw or transfixing wire were described. The two groups were compared with the each parameters. RESULTS: Fracture classifications were changed in 4 cases. The locations of main fragment were changed in 10 cases. Significant difference was noted in the amount of articular depression (p<0.05) but no significant difference was found in displacement (p=0.332) . Especially in Schatzker-VI cases, articular step offs over 2mm and articular fragments over 2x2cm were revealed in detail by MRI (p<0.05) . CONCLUSION: Fracture type, amount of depression, location, and fracture configuration can be better visualized by MRI, especially in highly comminuted plateau fractures (Schatzker-VI) . The MR imaging seems to be recommended as a primary mode for evaluation of plateau fractures, especially in Schatzker-VI fractures.
Classification ; Depression ; Humans ; Magnetic Resonance Imaging* ; Tibia