The purpose of this study was to verify the split-crest technique experimentally for successful implantation at alveolar bone having unfavorable condition. Using inferior border of the mandible of the canine, we made comparable study about the state of osseointegration between conventional technique and split-crest technique. We set experimental group which was implanted at inferior border of the mandible of the canine by split-crest technique using the fixture of 3.75 mm width and 8mm length, and set control group which was implanted by conventional technique at the counter area of the mandible. The experimental animal was sacrificed at 1, 4, 8 and 16 week. We observed the changing process of bone formation following implantation with stereoscopy, light microscopy, electron microscopy and fluorescent microscopy, and studied histomorphometrically. Histologic results were as follow : 1. In control group, a bit of new bone formation was initiated on a portion of bone defect area at 1 week. The initiation of osseointegration between fixture and new bone was seen at 4 week. New bone tissue with normal shape and structure formed and filled defect area at 8 week. But complete bone remodeling was attained at 16 week. 2. In experimental group, bone formation around fixture was going on actively. But the shape and structure of new bone area was more irregular than that of control group, and bone density was also lower than that of control group. Active new bone formation was still observed at 16 week. 3. The osseointegrated new bone was remodeled to cancellous bone having trabeculae and marrow space composed of compact lamellar bone. 4. In fluorescent microscopic analysis, active bone formation was seen between 4 weeks and 8 weeks at control group. Otherwise, along the all experimental period new bone formation was observed evenly at experimental group. 5. Both control and experimental group, normal osseointegration was accomplished without bone resorption which is essential factor in split-crest technique. As previous results, when implantation using split-crest technique at alveolar bone attenthaving unfavorable condition, early bone formation was delayed slightly, but we could get attentive results along the long term period.
Animals ; Bone and Bones ; Bone Density ; Bone Marrow ; Bone Remodeling ; Bone Resorption ; Mandible ; Microscopy ; Microscopy, Electron ; Osseointegration* ; Osteogenesis

No abstract available.
Humans ; Orthognathic Surgery ; Prognathism

No abstract available.
Mandibular Osteotomy*

No abstract available.
Skeleton

No abstract available.
Giant Cells ; Gingiva ; Granuloma, Giant Cell ; Osteomalacia

Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on screening anti-oral cancer activities among 14 traditional medicinal plant extracts that revealed anticancer activities on other solid tumors. The results were as follow: 1. Methanol extract of Lepidium apetalum showed the highest anti-oral cancer activity against A253 cells. At concentration of 4 microgram/ml, the cell viability was 48% under our experimental condition. IC50 value obtained was 4 microgram/ml. 2. Methanol extract of Coptis japonica and Solanum nigrum were effective on KB cells. Cell viability observed were 62% and 67% at concentration of 4 microgram/ml, and IC50 values were 12 microgram/ml and 10 microgram/ml respectively. 3. When the methanol extract of Lonicera caerule was combined with 2 microgram/ml of cisplatin, the anticancer activity was synergistically increased. One hundred microgram/ml of Lonicera caerule showed 92% (alone) or 59% (combined with cisplatin) cell viabilities. IC50 value of Lonicera caerule extract against KB cells was reduced from 301 microgram/ml to 126 microgram/ml when combined with 2 microgram/ml of cisplatin. 4. Medicinal plant extracts effective on both A253 and KB cells were Coptis japonica, Lepidium apetalum, Solanum nigrum, Caesalpiniae Lignum, Curcuma aromatica.
Bone Marrow ; Caesalpinia ; Cell Survival ; Cisplatin ; Coptis ; Curcuma ; Humans ; Inhibitory Concentration 50 ; KB Cells ; Lepidium ; Lonicera ; Mass Screening ; Methanol ; Mouth Neoplasms ; Plants, Medicinal* ; Solanum nigrum

No abstract available.
Adenoma, Pleomorphic ; Mouth Mucosa

No abstract available.
Facial Bones*

No abstract available.
Mediastinitis*

No abstract available.
Facial Bones*