Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta (E. hirta) in MCF-7 cell line model using comet assay.
Methods: The cytotoxicity of E. hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E. hirta was assessed by using Comet assay.
Results: Both toxicity tests exhibited significant toxicity result. In the comet assay, the E. hirta extract exhibited genotoxicity effects against MCF-7 DNA in a time-dependent manner by increasing mean percentage of DNA damage. The extract of E. hirta showed significant toxicity against brine shrimp with an LC50 value of 620.382 μg/mL (24 h). Comparison with positive control potassium dichromate signifies that cytotoxicity exhibited by the methanol extract might have moderate activity.
Conclusion:The present work confirmed the cytotoxicity and genotoxicity of E. hirta. However, the observed toxicity of E. hirta extracts needs to be confirmed in additional studies.

100 cases of gastritis verrucosa were classified into acute stage and chronic active stage according to the morpnological and histological characteristics.The CP positive rates in the above two stages were 97% and 68.7%,respectively,while that of the erosion area and the surrounding intact mucosa ' were 83.0% and 45.0%,respectively.The amount of CP was directly related to the degree of inflammatory cell infiltration and erosion.So VG was closely related to CP infection.In the chronic active stage,the detection rates of the Ig coated CP in the tissue section,serum IgA,IgG,and gastric juice IgA were all higher than those in the acute stage (P

OBJECTIVETo investigate the antioxidant activity of methanolic extracts of Lantana camara (L. camara) various parts and the determination of their total phenolics content.

METHODSThe extract was screened for possible antioxidant activities by free radical scavenging activity(DPPH), xanthine oxidase inhibition activity and Griess-Ilosvay method.

RESULTSThe results showed that all the plant parts possessed antioxidant properties including radical scavenging, xanthine oxidase inhibition and nitrites scavenging activities. The antioxidative activities were correlated with the total phenol. The leaves extract of L. camara was more effective than that of other parts.

CONCLUSIONSThis study suggests that L. camara extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

Allopurinol ; pharmacology ; Antioxidants ; pharmacology ; Chronic Disease ; drug therapy ; Free Radical Scavengers ; pharmacology ; Humans ; Lantana ; chemistry ; Methanol ; Oxidation-Reduction ; Oxidative Stress ; drug effects ; Phenols ; pharmacology ; Phytotherapy ; methods ; Plant Extracts ; pharmacology ; Plant Leaves ; chemistry ; Plant Roots ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Reactive Oxygen Species ; Solvents