[Objective] To study the inhibition effect of berberine on cell proliferation in acute T lymphocytic leukemia cell line Molt-4,and its mechanisms.[Methods] The logarithmic growing cell lines were incubated with 0,10,100 μg/ml berberine for various hours.The apoptosis induced by berberine was detected by flow cytometry,eletrophoresis and microscopy.The protein pro-Caspase-3 and p65 were determined by Western bolt assay.[Results]Treated with berberine,Molt-4 cells were found with apoptosis bodies by microscopy.By flow cytometry,it was found that number of G2/M cells was increased and number of S cells was reduced.Necrosis band was found in DNA ladder electrophoresis.The expression of pro Caspase-3 was activated partly and p65 was down-regulated in Molt-4 cells treated with berberine.[Conclusion]Berberine can induce Molt-4 cells apoptosis, and NF-κB、Caspase-3 have been shown to regulate the apoptosis programme.

Objective To observe the correlation between serum fatty specific serine protease inhibitor (vaspin) and inflammato-ry factors in the patients with coronary heart disease (CHD) .Methods 114 cases of CHD(CHD group) in our hospital from April 2010 to April 2015 were selected and divided into the stable angina group ,unstable angina group and acute myocardial infarction group according to the disease type ,and divided into the 1-vessel lesion group ,2-vessel lesion group and 3 or more vessel lesion group according to the coronary lesion vessels .At the same time 45 healthy persons were selected as the healthy control group .The enzyme-linked immunosorbent assay(ELISA) was adopted to detect the levels of vaspin ,tumor necrosis factor-α(TNF-α) ,interleu-kin (IL)-6 ,IL-10 and C reactive protein(CRP) .Results The vaspin level in the CHD group was significantly lower than that in the healthy control group ,while the levels of TNF-α,IL-6 ,IL-10 and CRP were significantly higher than those in the healthy control group ,the difference between the two groups was statistically significant (P<0 .05).The levels of vaspin in the stable angina pec-toris group ,unstable angina group and acute myocardial infarction group decreased in turn ,and the levels of vaspin in the 1-vessel lesion group ,2-vessel lesion group and 3 or more vessel lesion group decreased in turn ;the levels of CRP、IL-6、IL-10、TNF-αin the stable angina pectoris group ,unstable angina group and acute myocardial infarction group increased in turn ,and the levels of CRP、IL-6、IL-10、TNF-αin the 1-vessel lesion group ,2-vessel lesion group and 3 or more vessel lesion group increased in turn ;the differ-ence between the groups was statistically significant (P<0 .05) .The CHD severity was positively correlated with the levels of CRP , IL-6 ,IL-10 and TNF-α(P=0 .007 ,0 .003 ,0 .004 ,0 .005) ,and negatively correlated with the vaspin level (P=0 .002) .Conclusion The vaspin level is closely related to the inflammatory factors in CHD patients ,and is related with the vascular lesions severity of CHD .

Objective:To explore the comparison of imaging characteristics and diagnostic value between ultrasound and CT in patients with pulmonary tuberculosis in Tibetan population in plateau region.Methods:From May 2022 to November 2023,a total of 100 patients with pulmonary tuberculosis of Tibetan population in plateau region,who admitted to Qinghai Provincial Hospital of Traditional Chinese Medicine,were selected as the study objects.All of them were examined by ultrasound and CT.The ultrasonic imaging characteristics of the lesions of patients with pulmonary tuberculosis in plateau region were dynamically observed.And then,the diagnostic effects of ultrasound and CT on pulmonary tuberculosis in the Tibetan population in the plateau region were compared.Results:There was no significant difference in the positive rate of pulmonary tuberculosis in Tibetan population of plateau region between ultrasound and CT examinations(x2=0.421,P＞0.05).The diagnostic sensitivity,diagnostic specificity and Yoden index of ultrasound diagnosis were respectively 97.80%,44.44%and 42.24%,and these of CT diagnosis were respectively 96.77%,42.86%and 39.63%,and the differences were not significant(x2=0.184,0.004,0.272,P＞0.05),respectively.There was no significant difference in detection rate of the distribution of pulmonary tuberculosis lesions in Tibetan population in plateau region between ultrasound and CT examinations(x2=2.011,P＞0.05).There were no significant differences in the detection rates of atelectasis,cavity,calcification and accompany of pleural effusion between ultrasound and CT examinations in patients with pulmonary tuberculosis in Tibetan population in plateau region(x2=0.190,0.197,0.190,0.046,P＞0.05).Conclusion:There are no significant differences in positive detection rate,the detection rate of the distribution of diseased lung lobes,the detection rates of the complication of pulmonary tuberculosis or associated signs such as atelectasis,cavity,calcification and accompany of pleural effusion between ultrasound and CT examinations.The medical resource of plateau region is lack,which is shorter of large examination equipment.Therefore,using ultrasound examination to conduct general survey and preliminary diagnosis for pulmonary tuberculosis is more suitable to the plateau region that lacks CT equipment or is affected by electricity