OBJECTIVE:To compare the efficacy and safety of gio combined with oxaliplatin (SOX) versus 5-fluorouracil (5-FU)combined with calcium folinate and oxaliplatin(mFOLFOX6)in the treatment of diffuse advanced gastric cancer. METH-ODS:The data of 128 patients with diffuse advanced gastric cancer was retrospectively analyzed and patients were divided into SOX group(66 cases)and mFOLFOX6 group(62 cases)by different medication. SOX group received Gio capsule after breakfast and dinner,which was <1.25 m2,40 mg,1.25-1.5 m2,50 mg,>1.5 m2,60 mg,d1-14+130 mg/m2 Oxaliplatin for injection,intrave-nously,d1;3-week was regarded as a treatment course,the efficacy was evaluated every 2 courses,and it lasted a maximum of 8 courses but a minimum of 2 courses. mFOLFOX6 group received 85 mg/m2 Oxaliplatin for injection,intravenously,d1+200 mg/m2 calcium folinate,intravenously,d1+400 mg/m2 5-FU for injection by rapid intravenous injection,d1,then 2 400 mg/m2 5-FU,main-taining 46 h by continuous infusion. 2-week was regarded as a treatment course,the efficacy was evaluated every 3 courses,and chemotherapy was conducted in a maximum of 12 courses but a minimum of 3 courses. Clinical efficacy,time to progression,sur-vival time and incidence of toxicities in 2 groups were observed. RESULTS:The objective response rate,time to progression and median survival time in SOX group was significantly higher than mFOLFOX6 group,the difference was statistically significant (P<0.05 or P<0.01). There was no significant difference in the disease control rate and incidence of toxicities in 2 groups(P>0.05). CONCLUSIONS:The efficacy of SOX is superior to mFOLFOX6 in the treatment of diffuse advanced gastric cancer,it can prolong the survival time,with similar safety.

Objective To explore the influence of type A character on early renal impairment in elderly patients with essential hypertension and the protective effect of benazepril. Methods According to the type A behavior measuring scale, 80 cases with type A character and 80 cases without type A character were screened out from the elderly patients with essential hypertension. The patients received benazepril intervention with incipient dose of 10 mg daily, which increased gradually to the largest does of 20 mg daily within 1 month, The treatment lasted 6 months in order to make the patients achieve the normal blood pressure (BP). The general data, plasma angiotensin Ⅱ (AngⅡ )> urinary albumin excretion rate (UAER) and urinary β2-microglobulin (β2-MG) were measured at pretherapy and post-treatment respectively. Results There were no statistically significant differences at pretherapy between the two groups in age, gender, body mass index (BMI), blood urea nitrogen (BUN), creatinine (Cr), systolic pressure and diastolic pressure (all P>0.05). Ang Ⅱ [(60.3±11.6)mg/Lvs (55.8±10.2)mg/L], UAER[(83.3±23.3)μg/min vs. (75.7±16.3)μg/min] and β2-MG [(75.6±24.5)mmol/L vs. (66.0±19.4)mmol/L] were significantly higher in type A character group than in non-type A character group (t=2.598, 2.603, 2.742; P= 0.016,0.012, 0.008). TypeA character value of the two groups showed a positive correlation with Ang Ⅱ (r= 0.361, P=0.002), UAER (r=0.198, P=0.015) and β2-MG (r=0.202, P = 0.021). Only Ang Ⅱ (β=0.291, t=2.793, P= 0.008) was correlated to type A character value independently by multiple stepwise regression analysis. Before treatment in both of the type A character group and the non-type-A character group, there was a positive correlation between Ang II and UAER, (β2-MG, and obvious correlation in type-A character group. The systolic pressure, diastolic pressure, Ang Ⅱ , UREA and β2-MG were all decreased obviously in two groups post treatment. And the variable value of AngⅡ was also positively correlated with UAER (r=0.373, P=0.005; r=0.282, P=0.032), fr-MG (r= 0.385, P=0.003; r= 0.279, P=0.039) before and after treatment. Conclusions Type A character can aggravate injury of early renal function by certain stress systems in the elderly patients with essential hypertension and benazepril has better protective effect for it.

OBJECTIVE:To prepare Potassium dehydroandrographolide succinate(PDS)hollow suppository and establish a method for its quality control.METHODS:The PDS hollow suppository was prepared with semisynthesis fatty acid glyceride as ground substances and PEG300 or 1,2 propylene glycol as disperse medium.The content of PDS was determined by ultraviolet spectrophotometry at a detection wavelength of 251nm.RESULTS:The linear concentration range of PDS was 6.0～50.0?g?mL-1(r=0.999 9,n=5).The two kinds of PDS hollow suppository were all up to the quality standard.CONCLUSION:This method is reasonable in design and feasible in technology.

Objective To investigate the vision-related quality of life （VRQL） in patients with glaucoma and to explore the intervention. Methods 260 patients with primary open angle glaucoma (POAG) were investigated with questionnaire of VRQL and self-evaluation before and after health education. 100 health adults with similar age and vision was selected as controls. Results There were significant decrease on VRQL and self-evaluation in patients with glaucoma. Systematic health education can significantly improve their VRQL and self-evaluation. Conclusion The in patients with Glaucoma may result in decrease of VRQL， and systemic health education can improve the quality of life.

Objective To construct and express human ScFv against keratin in E.coli,and identify its binding activity with antigens and antigenic specificity.Methods By genetic engineering technology,human anti-keratin ScFv was constructed from Fab fragment selected from established semi-synthetic phage antibody library.The binding activity with antigens and antigenic specificity of expressed products were i-dentified by ELISA.Results Results of DNA sequence analysis showed that nucleotide sequence of V?and VH of ScFv gene was similar to that of V?and VH of Fab gene,suggesting that there was no mutation in the construction of ScFv.The soluble anti-keratin ScFv was found to have a good antigenic specificity as well as excellent binding activity with target antigens by ELISA.However,the binding activity of ScFv with keratin was lower than that of Fab.Conclusions The successful expression and identification of human an-ti-keratin ScFv might lay a solid foundation for further observation of biological activities of anti-keratin au-toantibody,manufacture of genetic-engineering anti-keratin products,and promotion of clinical application of genetic-engineering anti-keratin antibodies.

Aim To observe the production of anti-idiotypic antibodies directed against AK auto Ab in the rabbit sera after long-term, high dose allogenic AK auto Ab injection. Methods Allogenic AK auto Ab (5mg/kg)was injected intramuscularly to rabbit once every other day for 90 days. Anti-idiotypic antibodies in rabbit sera were detected by ELISA. Results Rabbits injected with AK auto Ab generated anti-idiotypic antibodies that could react to F(ab′ )2 of AK auto Ab. The titers of anti-idiotypic antibodies reached the highest levels at 4 weeks after the administration of AK auto Ab and then gradually decreased. Conclusion Rabbits could be induced to form immune tolerance when high-does allogenic AK auto Ab is administrated for long-term.

Objective To study the therapeutic effects of Bioenterics Intragastric Balloon (BIB) on obesity under gastroscopy.Methods Data of 47 patients treated with Bioenterics Intragastric Balloon under gastrscopy were reviewed from July,2010 to May,2011.Results Weight loss ( mean 15.4 kg ) was successfully achieved in all the patients during 6 months.BMI decreased by 3.2-6.4 kg/m2 ( mean 4.7 kg/m2 ).There was no serious side effect with a better result for obesity according to the follow-up.Conclusion BIB is effective for obesity for noninvasiveness,stable speed of weight loss and less pain.

[Summary] Four patients with hyperthyroid-associated exophthalmos, myxedema, acropachy ( EMA ) syndrome, including three male patients and one female patient were diagnosed with Graves′diseases and treated by 131 I therapy. Complaints of thyrotoxicosis were presented at the onset. Tibia myxedema and acropathy appeared, and eye symptoms aggravated in two patients after anti-thyroid drug therapy and 131 I therapy. Four cases were all given clobetasol propionate, miconazole nitrate, neomycin sulfate and urea cream alone or in combination with compound betamethasone local injection treatment, and three cases were given low-dose oral prednisone treatment. Complaints of tibia myxedema and eye symptoms were significantly improved after the treatment. Therefore, we should be wary of the occurrence of hyperthyroid-associated EMA syndrome after 131 I therapy. Corticosteroid might be the effective therapy for myxedema and eye symptoms of EMA syndrome.

Objective To validate the antiviral effect of oxymatrine against hepatitis C virus (HCV) in the construction of the cell culture-based infectious virus system HCVcc.Methods An HCVcc system was established by infecting Huh7.5.1 cells with the J6 adapted virus (J6cc).Cells of the HCVcc system were then treated with different concentrations of oxymatrine for 24 h,48 h and 72 h,or left untreated (controls).MTT assay was used to detect effects on proliferation.Real-time quantitative PCR was used to detect effects on HCV mRNA expression level,and immunofluorescence was used to detect effects on HCV protein expression.Data were expressed as mean±standard deviation,and statistically assessed using one-way ANOVA and one-sample t-test.Results Treatment with 2,4,8 and 12 mg/mL of oxymatrine for 24 h inhibited proliferation of the J6cc-infected cells by 8.4％,15.2％,29.6％ and 48.6％ respectively,48 h treatment inhibited by 14.3％,25.7％,46.1％ and 66.4％ respectively,and 72 h treatment inhibited by 36.5％,46.6％,70.6％ and 85.4％ respectively.Thus,the effects of oxymatrine were time-and dose-dependent (P ＜ 0.05).The mRNA expression level in the oxymatrine-treated cells of the HCVcc system was 0.59 ± 0.12,which was significantly lower than that in the control cells (P ＜0.05).Moreover,as the oxymatrine concentration increased from 2 mg/mL to 12 mg/mL,the expression levels of HCV proteins also showed a decreasing trend.Conclusion We successfully constructed a J6cc infection HCVcc system and verified the antiviral effect of oxymatrine against HCV.

Objective:To evaluate the early side effects and influencing factors in patients undergoing intensity-modulated radiation therapy after cervical cancer surgery.Methods:A retrospective analysis of 106 patients with cervical cancer who underwent postoperative intensity-modulated radiation therapy in our hospital from January 2014 to December 2017 was conducted. The occurrence of toxic and side effects were summarized, and the related clinical indicators were analyzed using univariate and multivariate analyses.Results:The 3-year overall survival rate was 88.1%, and the 3-year progression-free survival rate was 84.8%. The incidence of acute hematologic toxicity greater than grade 3 was 33%. The incidence of early intestinal reactions was 35%, of which only 1.8% was greater than grade 3. Univariate analysis demonstrated that severe acute hematologic side effects were statistically significant ( χ2=1.789-17.895, P<0.05) with the level of hemoglobin before radiotherapy, radiation in the abdominal aortic lymphatic drainage area or not, an increase of local radiotherapy dose in the target area, and chemotherapy or not. Multivariate analysis showed that concurrent chemotherapy significantly increased the incidence of severe acute hematologic toxicity ( χ2=3.929, P<0.05). Conclusions:In patients with cervical cancer undergoing postoperative intensity-modulated radiation therapy, grade 3 and greater toxic side effects are mainly related to hematological toxicity, and the incidence of severe bowel toxicity in the early stage is low.