1.Study on metabolites of ferulic acid and gallic acid compatible with danggui and chishao in rat plasma and urine.
Wen DING ; Dawei QIAN ; Jin'ao DUAN ; Erxin SHANG ; Yuping TANG ; Yefei QIAN ; Niancui LUO
China Journal of Chinese Materia Medica 2012;37(3):366-372
OBJECTIVETo study the major metabolites of ferulic acid and gallic acid compatible with Danggui Chishaoyao in rat plasma and urine.
METHODThe metabolites of ferulic acid and gallic acid in rat plasma and urine were analyzed after oral administration of compatible Danggui Chishaoyao using UPLC-Q-TOF-MS.
RESULTOn the basis of the mass information, it was inferred that in vivo metabolites of ferulic acid were be in the form of methylation products, sulfate conjugation products and glucuronidation conjugation products and so on; meanwhile, gallic acid was mainly transformed into eduction products and methylation products.
CONCLUSIONThere are kinds of phase I and phase II metabolites of ferulic acid and gallic acid in rat plasma and urine, which provide a basis for its efficacious materials and action mechanism.
Animals ; Coumaric Acids ; blood ; metabolism ; urine ; Drugs, Chinese Herbal ; metabolism ; Gallic Acid ; blood ; metabolism ; urine ; Herb-Drug Interactions ; Male ; Metabolome ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
2.Evaluation of the intervention effect of conventional and peer AIDS voluntary counseling and testing services among young students
Chinese Journal of School Health 2023;44(10):1468-1472
Objective:
To explore the influence of two voluntary counseling and testing(VCT) services on young students acquired immune deficiency syndrome(AIDS) related knowledge and behaviour, so as to provide a new idea for further improvement and promotion of VCT services.
Methods:
From April 2017 to December 2018, the sample size,selected from 12 patrol and fixed monitoring sites in colleges in Guangzhou, was calculated by non inferiority trial, and the students who received services at the same period were 1∶1 matched. A total of 113 students received conventional VCT and 186 recerived peer VCT services. The Chi square test was used to analyse the difference of AIDS related knowledge and behavior before and after intervention, and a survey was conducted on satisfaction.
Results:
After young students received conventional VCT services, the overall awareness rate of AIDS basic knowledge increased from 80.53% to 93.75%, and the frequency of condom use every time during heterosexual sexual activity in the past year increased by 29.17 percentage points ( χ 2=4.49,4.10, P <0.05). After young students received peer VCT services, the awareness rate of the Four Frees and One Care Policy increased from 34.95% to 58.26%, the rates of homosexual anal sex and more than or equal to 2 sexual partners in the past half a year decreased by 14.84 and 29.43 percentage points, respectively( χ 2=15.69, 4.82, 10.97, P <0.05).
Conclusion
After young students receive two modes of VCT services respectively, the AIDS related knowledge and behavior have been improved, and the influence of the two VCT services has different advantages. The combination of conventional VCT and peer education might be a more effective way of AIDS intervention.
3.Influence of intrahepatic cholestasis during pregnancy on the incidence of preterm birth
Maimaiti REYIHANGULI ; Haiyan LIU ; Wenhui HAN ; Yefei LUO ; Anqun HU ; Yan WANG ; Haili WANG ; Haiyan XIONG ; Yingjie ZHENG
Chinese Journal of Epidemiology 2017;38(10):1415-1418
Objective Intrahepatic cholestasis during pregnancy (ICP) and its relation to incidence of preterm birth (PTB) were under study.Methods A prospective cohort study was carried out that including all the hospitalized pregnant women with live singleton births,from January 2014 to March 2015 in Anqing Municipal Hospitals.Informed consent was followed in every pregnant woman with related demographic information collected through questionnaire and hospital electronic medical record system.Both univariate and multi-variate statistical methods were used to analyze the relations between ICP and incidence of PTB.Results A total of 2 758 pregnant women were included in this study.The incidence proportions of ICP and PTB appeared as 7.25% and 16.28% respectively.Results from the logistic regression analysis showed that ICP increased the risk of both overall PTB (RR=2.33,95%CI:1.67-3.25) and medically indicated PTB (RR=8.46,95%CI:5.45-13.12),but not the spontaneous PTB (RR=0.94,95%CI:0.57-1.54).Conclusion ICP seemed to have increased the risk on medically indicated PTB but not the spontaneous PTB.
4.Comparison of results of two immunoassays for detection of hepatitis B surface antigen in pregnant women
Haiyan XIONG ; Yefei LUO ; Haiyan LIU ; Wenhui HAN ; Anqun HU ; Yan WANG ; Yingjie ZHENG
Chinese Journal of Epidemiology 2017;38(11):1537-1540
Objective To evaluate and compare the detection consistency of hepatitis B surface antigen (HBsAg) by two immunoassays: enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescent immunoassay (ECLIA). Methods A prospective study was conducted among 2296 pregnant women recruited consecutively from January 1, 2014 to January 31, 2015 in a hospital. Blood samples were collected from them for the detection of HBsAg by using ELISA and ECLIA, Ka ppa test was performed on the results. Nested polymerase chain reaction and sequencing of HBV S gene were also performed in all samples. Phylogenetic analysis was performed using Mega 6.0 software. Results The two methods had high detection consistence of HBsAg ( Ka ppa=0.71). There were significant differences in detection result of B genotype and adw2 serotype HBV strains between two methods. Among 123 identified HBV strains, 113 belonged to genotype B and available for further analysis. The difference in detection of substitution rates between two methods or different positive groups were not significant. Compared with ELISA single positive group, the ECLIA single positive group had completely different substitution sites. Conclusion The two methods had high detection consistence of HBsAg, but there were still 32.4% HBV DNA positive cases in ELISA/ECLIA single positive group, and complete complementary substitution sites between ELISA single positive group and ECLIA single positive group. Our results suggested that more effective detection procedure should be considered for the possible impact of the HBV silent transmission and infection.
5. Applicability and feasibility of "Lingnanzhun" -an "Internet Plus-based HIV Self-testing Tool" targeting MSM in Guangzhou
Yanshan CAI ; Yuzhou GU ; Fei ZHONG ; Lirui FAN ; Yuteng ZHAO ; Yefei LUO ; Yongheng LU ; Weiyun HE ; Weibin CHENG ; Huifang XU
Chinese Journal of Epidemiology 2019;40(10):1212-1216
Objective:
This article was to evaluate the applicability and feasibility of "Lingnanzhun" -an "Internet Plus-based HIV Self-testing Tool" targeting MSM in Guangzhou. Hopefully, the results could be used to improve the existing HIV testing services and to support the implementation and scale-up of HIV self-testing programs.
Methods:
Data were collected from a survey on HIV testing preferences among the Internet-using MSM in April to June, 2015. Univariate and multivariate logistic regression analysis were applied to identify factors associated with the use of HIV self-testing service provided by Lingnanzhun. Information related to the users of Lingnanzhun during September 2014 and December 2018 was also collected.
Results:
769 MSM were recruited as participants. Of them, age distribution was 16-77(28.6±6.8) years old, 88.3
6. Characteristic analysis among MSM-users of the "Online HIV Acquisition Risk Assessment System" in Guangzhou
Yefei LUO ; Yuzhou GU ; Fei ZHONG ; Huifang XU ; Yanshan CAI ; Lirui FAN ; Yuteng ZHAO ; Zhigang HAN ; Weiyun HE ; Gang MENG ; Xiaofei JIA ; Weibin CHENG
Chinese Journal of Epidemiology 2019;40(10):1217-1221
Objective:
To analyze the characteristics of levels related to the risk through self-evaluation system, among MSM users in Guangzhou, between 2015 and 2017.
Methods:
Between 2015 and 2017, data was collected from the users of a self-evaluation system network related to HIV infection, based on the previous 'HIV health risk appraisal model’. Information on risk factors was collected to calculate the scores and levels of risks and to estimate the incidence of HIV. Taking the reference of
7. Effectiveness of the "Internet Plus-based AIDS Comprehensive Prevention Service System" among MSM in Guangzhou
Yuzhou GU ; Weibin CHENG ; NgaiSie WONG ; Fei ZHONG ; Yanshan CAI ; Yefei LUO ; Zhigang HAN ; Weiyun HE ; Qi LIU ; Jiewei LIU ; Huifang XU
Chinese Journal of Epidemiology 2019;40(10):1222-1226
Objective:
To evaluate the effectiveness of the "Internet Plus-based AIDS Comprehensive Prevention Service System" among men who have sex with men in Guangzhou.
Methods:
Data through case-reporting and follow-up programs on MSM HIV/AIDS in Guangzhou was collected from the China Information System for Disease Control and Prevention, which including those from the referral and follow-up treatment compliance programs in 2008-2014 (pre-treatment) and 2017-2018 (post-treatment). According to the types of care services, three groups were set as: with "Internet Plus" service, with 'HIV counseling/testing service’ or with 'routine medical service’. General Estimating Equation (GEE) was used to analyze the follow up situation of HIV/AIDS cases, annually. Cox proportional hazard regression model was used to analyze the proportions of treatment referral, within the 30 days of diagnosis.
Results:
Before the implementation of immediate treatment after HIV diagnosis, 90.6
8. Status quo and characteristic analysis among MSM-users of the "Internet Plus-based AIDS Comprehensive Prevention Service System" in Guangzhou
Weibin CHENG ; Shunming LI ; Yuzhou GU ; Fei ZHONG ; Wenting HUANG ; Yefei LUO ; Yanshan CAI ; Weiyun HE ; Lirui FAN ; Yuteng ZHAO ; Huifang XU ; Weiming TANG
Chinese Journal of Epidemiology 2019;40(10):1206-1211
Objective:
To analyze the characteristics of the "Interner Plus-based AIDS Comprehensive Prevention Service System" among MSM who frequently using the Internet in Guangzhou.
Methods:
An online survey was conducted among MSM who were recruited through gay-website portals between August and September, 2018 in Guangzhou, to collect information regarding the use of and attitudes on the "Interner Plus-based AIDS Comprehensive Prevention Service System" . Logistic regression was used to explore the association between the use of Internet intervention tools and related behavioral characteristics. Information on the awareness of AIDS, HIV testing, and condomless anal sex behavior were compared between the core or non-core services users.
Results:
A total of 777 Internet-based MSM were recruited as participants including 638 men (82.1