Objective To observe the clinical effect of cardiopulmonary resuscitation (CPR) in the treatment of cardiac arrest. Methods A retrospective analysis was coducted, 137 patients with cardiac arrest admitted to Department of Emergency of Fuyang People's Hospital from January 2017 to December 2018 were enrolled, emergency rescue with CPR machine. There were 116 cases of CPR in emergency room, including 44 cases of cardiac arrest before hospital and 72 cases of cardiac arrest in hospital. There were CPR was 21 cases of CPR in EICU. The difference of return of spontaneous circulation (ROSC) rate and rescue time between Emergency Room and Emergency Intensive Care Unit (EICU) and ROSC rate in patients with cardiac arrest before and after emergency rescue room were compared. Results There were 45 cases of ROSC in 137 patients, the success rate of rescue was 32.85%, rescue time was 30.00 (20.00-40.00) minutes, and the maximum duration of times was 180 minutes. The ROSC rate of EICU was significantly higher than that of emergency room [66.67% (14/21) vs. 26.72% (31/116), P < 0.05], and the rescue time was significantly shorter than that of emergency room [minutes: 25.00 (10.00-30.00) vs. 30.00 (25.00-40.00), P < 0.05]. The ROSC rate of patients with cardiac arrest in emergency room was significantly higher than that before hospitalization [33.33% (24/72) vs. 15.91% (7/44), P < 0.05], there was no significant difference in rescue time between patients with cardiac arrest in emergency room and that before hospitalization [minutes: 30.00 (20.00-40.00) vs. 30.00 (26.25-40.00), P > 0.05]. Conclusions The function of CPR provides high quality extrathoracic compression, which effectively saves the labor cost of cardiac compression, especially in the case of shortage of medical and nursing staff. In the face of patients who need continuous CPR for a long time, it is a feasible choice to use CPR machine instead of unarmed CPR, which is worth popularizing in clinic.

AIMTo explore the influence of hyperbaric oxygen (HBO) preconditioning on anoxic resistance and anti-weariness at high altitude.

METHODS(1) SOD, MDA, NO, NOS, BLA and BUN of 20 youths living at 3 700 m altitude for half year were tested, then they were divided into group A (n=10, received HBO pretreatment twice) and group B (n=10, received HBO pretreatment 5 times) randomly. They were asked to pedal the EMG-bicycle-ergometer at the second and eighth day, and then the same items were tested. (2) 29 youth who would go to Astronomical Spot (5200 m) were randomly divided into group HBO (n=11, received HBO pretreatment once per day for 2 days at Yecheng (1400 m)) and comparison group (n=10). When they reached I Astronomical Spot, thematic biochemical index were investigated. (3) When 20 youth reached Thirty Milepost Barracks (3700 m) at the second day in their way to Immortal Gulf (5380 m) from Yecheng were randomly divided into group HBO (n=10, received HBO pretreatment once per day for 3 days) and comparison group (n=10). When they reached Immortal Gulf, the thematic biochemical index were investigated.

RESULTS(1) SOD, NO, NOS were increased and BLA, BUN, MDA were decrease in group A compared with that in group B until the eighth day, there was significant difference (P < 0.01). (2) SOD, NO, NOS were increased and BLA, BUN, MDA were decrease in group HBO compared with that in comparison that in group, there was significant difference between groups (P < 0.01, or P < 0.05).

CONCLUSIONHBO could enhance the activity of anti-oxidase and the cleared ability of lactic acid, and the effect of anti-weariness could last for 8 days.

Altitude ; Altitude Sickness ; prevention & control ; Fatigue ; prevention & control ; Humans ; Hyperbaric Oxygenation ; methods ; Hypoxia ; physiopathology ; Ischemic Preconditioning ; methods ; Male ; Oxidative Stress ; drug effects ; physiology ; Superoxide Dismutase ; metabolism ; Young Adult

The concept and framework of network toxicology and network toxicology of traditional Chinese medicine has been proposed in this paper. The related tools and technologies have been briefly introduced, and the prospects for network toxicology of traditional Chinese medicine are forecasted.
Animals ; Drug Interactions ; Drugs, Chinese Herbal ; analysis ; toxicity ; Humans ; Medicine, Chinese Traditional

The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is spread primary via respiratory droplets and infects the lungs. Currently widely used cell lines and animals are unable to accurately mimic human physiological conditions because of the abnormal status of cell lines (transformed or cancer cells) and species differences between animals and humans. Organoids are stem cell-derived self-organized three-dimensional culture in vitro and model the physiological conditions of natural organs. Here we showed that SARS-CoV-2 infected and extensively replicated in human embryonic stem cells (hESCs)-derived lung organoids, including airway and alveolar organoids which covered the complete infection and spread route for SARS-CoV-2 within lungs. The infected cells were ciliated, club, and alveolar type 2 (AT2) cells, which were sequentially located from the proximal to the distal airway and terminal alveoli, respectively. Additionally, RNA-seq revealed early cell response to virus infection including an unexpected downregulation of the metabolic processes, especially lipid metabolism, in addition to the well-known upregulation of immune response. Further, Remdesivir and a human neutralizing antibody potently inhibited SARS-CoV-2 replication in lung organoids. Therefore, human lung organoids can serve as a pathophysiological model to investigate the underlying mechanism of SARS-CoV-2 infection and to discover and test therapeutic drugs for COVID-19.
Adenosine Monophosphate/therapeutic use* ; Alanine/therapeutic use* ; Alveolar Epithelial Cells/virology* ; Antibodies, Neutralizing/therapeutic use* ; COVID-19/virology* ; Down-Regulation ; Drug Discovery ; Human Embryonic Stem Cells/metabolism* ; Humans ; Immunity ; Lipid Metabolism ; Lung/virology* ; RNA, Viral/metabolism* ; SARS-CoV-2/physiology* ; Virus Replication/drug effects*