1.Identification of phylloplane yeasts from paper mulberry (Broussonetia papyrifera (L.) L'Hér. ex Vent.) in Java, Indonesia
Dalia Sukmawati ; Ariyanti Oetari ; Dian Hendrayanti ; Mega Atria ; Wellyzar Sjamsuridzal
Malaysian Journal of Microbiology 2015;11(4):324-340
Aims: Broussonetia papyrifera (Saeh plant) has many qualities, the inner bark was the material for ‘dluwang’ papers on
which Indonesian historical manuscripts were written, and the leaves have bioactive constituents of medicinal value, and
antifungal activities. We investigated the diversity of yeast species associated with leaves from 6 months and 1.5 yearold
plants, which is prerequisite to understand the phylloplane yeasts and plant interaction.
Methodology and results: The yeasts were isolated from fresh leaves by washing and membrane filtration methods. A
total of 16 leaf samples of 6 months and 1.5 year-old plants were collected from four locations in Java, Indonesia, and
2,543 yeast isolates were obtained. Based on similarity of colony morphology, 82 representative yeast isolates were
selected and identified based on the sequence analyses of internal transcribed spacer (ITS) regions of rDNA. The
identification result showed that they consisted of 17 genera and 32 species. Thirty six of representative yeast isolates
belong to 11 genera (18 species) of the phylum Ascomycota and forty six isolates belong to 6 genera (14 species) of the
phylum Basidiomycota. Phylogenetic trees showed that the yeast isolates are phylogenetically diverse and distributed in
the phyla of Ascomycota (classes Saccharomycetales and Dothideomycetes) and Basidiomycota (classes
Microbotryomycetes, Tremellomycetes, and Ustilaginomycetes).
Conclusions, significance and impact of study: The phylloplane yeasts of B. papyrifera (Saeh plant) were
taxonomically heterogeneous. This is the first report of the isolation and identification of phylloplane yeasts from B.
papyrifera. Phylloplane yeasts may possess antagonistic activity to fungal plant pathogens in their natural habitats.
Yeasts
2.Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts.
Jung Hyun BYUN ; A Reum YU ; Myung Sook KIM ; Kyungwon LEE
Annals of Laboratory Medicine 2018;38(5):487-489
No abstract available.
Yeasts*
3.Production of Ginsenoside-Rg3 from Lipomyces starkeyi Grown on Ginseng-Steaming Effluent.
Jeong Hoon JANG ; Na Mi KIM ; Jong Soo LEE
Mycobiology 2010;38(2):153-155
To produce ginsenoside-Rg3 enriched yeast from ginseng-steaming effluent (GSE), Lipomyces starkeyi, which tends to grow well in GSE, was cultured in sterilized GSE and its growth and production of ginsenoside-Rg3 were determined. Growth of L. starkeyi was 86.1 mg per g GSE and its ginsenoside-Rg3 contents was 0.013 mg per g GSE.
Lipomyces
;
Yeasts
4.Determination of yeast diversity in fermented Sumbawa mare’s milk using internal transcribed spacers (ITS) fragment analysis
Ajeng Mareta Astiyani ; Yoga Dwi Jatmiko
Malaysian Journal of Microbiology 2022;18(4):398-407
Aims:
Molecular identification of yeast has been conducted on various fermentation products. However, the identification of yeast in fermented Sumbawa mare’s milk based on the genotyping method has not been carried out. This study was aimed to determine the diversity profile of yeasts in fermented Sumbawa mare’s milk using phenetic characters and PCR-RFLP analysis technique based on the ITS region.
Methodology and results:
Yeast isolates were phenotypically characterized and visualized in a dendrogram using CLAD97 software. Then, the yeast DNA was extracted using heat treatment and amplified using ITS1 and ITS4 primers. The amplicons were analyzed by RFLP using HindIII and HaeIII enzymes. The phylogenetic tree was constructed using MEGA 7.0. Based on the result of grouping by phenetic analysis and PCR-RFLP, the 12 isolates were divided into four groups with different members. The results of the phenetic analysis were divided into group I (all isolates of Dompu), group II (isolate B3, B4, S3), group III (isolate B5) and group IV (isolate S1). The types of yeast that were identified molecularly and represented each group of PCR-RFLP results included in group I were Kluyveromyces marxianus D1A and K. marxianus D1B, group II: K. marxianus D7, group III: Kazachstania humilis D4, while milk from Bima and Sumbawa has one yeast species as a member of group IV, namely Pichia kudriavzevii B3. Kluyveromyces marxianus was the yeast frequently found in Sumbawa fermented mare’s milk.
Conclusion, significance and impact of study
Various yeast species as a consortium of the milk samples can contribute to the increasing quality of fermented Sumbawa mare’s milk.
Yeasts
;
Koumiss
5.Presumptive Identification of Candida Species Using Candida ID.
Korean Journal of Clinical Microbiology 2003;6(2):144-148
BACKGROUND: Candida ID is introduced as a new chromogenic medium that allows presumptive identification of Candida species. We evaluated this medium to identify Candida spp. isolated from clinical specimens. METHODS: A total of 200 yeast isolates from clinical specimens in Ewha Womans University Mokdong Hospital, from April 2001 to August 2001 were identified by using the Vitek YBC (Hazelwood, Mo., USA). The results were compared with those by Candida ID (bioMeriux, Marcy l'Etoile, France). RESULTS: Candida ID correctly identified 98.0% of Candida spp. including 100% of C. albicans and 98.9% of C. tropicalis compared with the Vitek YBC. Among 84 strains showing blue colored colony on Candida ID, 82 strains (98%) were correctly identified as C. albicans but 2 strains were identified as C. glabrata and C. guilliermondii by the Vitek YBC. Among 92 strains showing pink colored colony, 90 strains (98%) were identified as C. tropicalis, and 2 strains were identified as C. guilliermondii and C. lusitaniae by the vitek YBC. CONCLUSIONS: Candida ID provides a more rapid and easier presumptive identification of major Candida spp. isolated from clinical specimens.
Candida*
;
Female
;
Humans
;
Yeasts
7.Clinical effects of yeast derived recombinant methionyl growth hormone in growth hormone deficiency.
Journal of the Korean Pediatric Society 1991;34(3):334-339
No abstract available.
Growth Hormone*
;
Yeasts*
8.Clinical effects of yeast derived recombinant methionyl growth hormone in growth hormone deficiency.
Journal of the Korean Pediatric Society 1991;34(3):334-339
No abstract available.
Growth Hormone*
;
Yeasts*
9.Malassezia Species Cultured From the Lesions of Seborrheic Dermatitis.
Yang Won LEE ; Ho Jung KANG ; Kyu Joong AHN
Korean Journal of Medical Mycology 2001;6(2):70-76
BACKGROUND: Malassezia yeasts were suggested to be one of the etiologic factors of seborrheic dermatitis. The genus Malassezia was recently revised into seven species. OBJECT: The study was done to classify the Malassezia yeasts cultured from the lesions of seborrheic dermatitis into one of the revised species and to find out the relationship between certain species of Malassezia and seborrheic dermatitis. METHODS: The specimen obtained from the lesions (face) of twenty cases of seborrheic dermatitis were cultured on Leeming & Notman's media. The obtained strains were placed into one of the seven species by their colony morphologies, microscopic morphologies and physiological characteristics. RESULTS: M. restricta occupied most (more than seventy percents) of the cultured colonies in 13 cases, M. globosa in 6 cases and M. furfur in 1 case respectively. CONCLUSION: M. restricta could be considered to be mostly implicated species in the seven revised Malassezia species in the lesions of seborrheic dermatitis of the face.
Dermatitis, Seborrheic*
;
Malassezia*
;
Yeasts
10.Wangiella Dermatitidis Infections: A Paradigm of the Opportunistic Mycoses Caused by Black Yeasts and Moulds.
Korean Journal of Medical Mycology 2000;5(2):45-50
No abstract available.
Exophiala*
;
Mycoses*
;
Yeasts*