Objective To explore association between the changes of myenteric nerve plexus and the delayed gastrointestinal transit in acute necrotic pancreatitis (ANP) rat complicated with hyperlipemia. Methods Atotal of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups (normal control group, ANP group, hyperlipemia (HL) control group and HL with ANP group). HL rat model was established by fed with high-fat diet for 4 weeks, and ANP rat model was induced by retrograde injection of 3.5% sodium taurocholate into pancreatic duct. Gastrointestina1 transit distance was measured by ink gavage method. The histological changes of cholinergic and nitriergic nerves in myenteric plexus were observed by Karnovsky-Root method and NADPH histochemistry method. Results Pathological injuries were more severe in HL with ANP group than in ANP group (12.8±0.63 vs. 10.8±1.93,P<0.01), gastrointestinal transit was obviously delayed (transmission rate was 27%±5% vs. 38%±6%,P<0.01), the density of cholinergic neurons decreased (4.80±1.23 vs. 5.80±0.79, P<0.05), and the density of nitriergic neurons significantly increased (8.70±0.75 vs. 6.80±1.48, P<0.01). There was a linear regression between changes of cholinergic and nitrievgic nerves in myenteric nerve plexus and gastrointestinal transit (R2=0.531, P<0.01). Conclusion There was significant gastrointestinal motility disorder in the ANP rat complicated with hyperlipemia, which may be closely related with the changes of myenteric nerve plexus.

Objective To explore the effect of Buyanghuanwu decoction on blood glucose control and renal function protection in patients with early diabetic nephropathy.Methods 170 cases of patients with early diabetic nephropathy from May 2012 to June 2013 in wenzhou hospital of integrated traditional chinese and western medicine were selected,all patients were divided into observation group and control group,the control group was given routine treatment,the observation group treatment with Buyanghuanwu decoction based on control group .The clinical effective rate was compared between the two groups after treatment,the levels of blood lipids, 24 h urine microalbumin and hemorheology were compared between the two groups before and after treatment.Results After treatment, the total effective rate of the observation group was 90.59%,which was significantly higher than the control group 76.47% (P<0.05).Compared with before treatment,the levels of triglycerides and cholesterol decreased in two groups,high-density lipoprotein levels increased,24 h urine microalbumin decreased,whole blood viscosity,whole blood viscosity trimming and fibrinogen decreased,the difference was statistically significant(P<0.05).Compared with the control group,the levels of triglycerides and cholesterol in observation group was lower,high-density lipoprotein was higher,24 h urine microalbumin was lower,whole blood viscosity,whole blood viscosity trimming and fibrinogen were lower,the difference was statistically significant(P<0.05).Conclusion Buyanghuanwu decoction in the treatment of diabetic nephropathy is effective in treating diabetic nephropathy,it can effectively reduce blood lipids and blood viscosity,significantly reduce urinary protein excretion and protect the kidneys.

Objective To evaluate the application of M2A capsule endoscopies ( CE) in the diagnosis of digestive tract diseases. Methods Forty - five patients (26 males, 19 females) , suffered from gastrointestinal bleeding, abdominal pain or chronic diarrhea were referred to this study and underwent capsule endoscopies. Their average age was 47. 8 years old (a range of 15 - 78). Capsule endoscopies were performed with the Given M2A video capsule system. The conventional diagnostic procedures (including small bowel radiograph, gastroenteroscopy, colonoscopy, etc. ) were done in 31 patients before or after the CE, thus the results of both procedures were compared. Results Among 45 patients, 44 have completed CE successfully. Any complication never experienced. The quality of photos was excellent. The significant pathological findings were revealed in 37 patients, diagnostic yield was therefore 84. 1% . Patients with esophagogastric diseases, small bowel diseases and colon diseases were found in 5,29 and 3 cases respectively. Thirty -one patients received a conventional diagnostic procedure before or after CE. Twenty one of them suffered from GI bleeding, pathological changes were found in 17 patients by capsule endoscopies, only 4 patients by conventional procedure; five of them suffered from abdominal pain, pathological changes were found in 5 patients by CE, only 2 patients by conventional procedure; 4 of them suffered from chronic diarrhea, pathological changes were found in 4 patients by CE, only 1 patient by conventional procedure. The pathological sites located by CE were quite similar to those by conventional diagnostic work-up. Conclusion CE has the advantages of high safety, excellent visualization and precise localization. Its efficiency in diagnosis is much better than that of conventional procedure, and is indispensable in the diagnosis of digestive tract diseases.

Objective To change the two-tiered C/S architecture of traditional PACS.Methods Based on the deeply research of PACS architecture,combination with DICOM and distributed object technology,propose distributed PACS system architecture,the three-tire client/server framework of distributed object technology introduced into the architecture of PACS to design the new architecture.Results In comparison with traditional PACS that were based on DICOM networks,the distributed PACS were improved themselves in system architecture and performance,and PACS were more open and interoperable.Conclusion The new architecture not only enhances the performance of PACS,but also meets the need of open and high interconnectivity and interoperability.

ObjectiveTo investigate the rule and characteristics of herbs used by Professor YAN Zheng-hua to treat digestive common diseases.Methods253 prescriptions of treating digestive diseases were searched.Medications for stomach ache,epigastric fullness,abdominal pain,diarrhea,constipation were analyzed by data mining and statistic method.ResultsThe common medications for stomach ache include Dried tangerine peel,Cyperus rotundus,White peony root and the most commonly used two-drug compatibility is “Dried tangerine peel and white peony root” (65.85％) ; common medications for epigastric fullness include Dried tangerine peel,Amomum villosum,Cyperus rotundus and the most commonly used two-drug compatibility is “Dried tangerine peel and Amomum villosum” (64.02％) ; common medications for abdominalpain include Tuckahoe,Dried tangerine peel,Red peony root and the most commonly used two-drug compatibility is “Tuckahoe and Dried tangerine peel” (50％) ; common medications for diarrhea include Tuckahoe,fried Malt,fried Grain buds and the most commonly used two-drug compatibility is “Tuckahoe and Atractylodes macrocephala” (67.5％) ; common medications for constipation include Radix Polygoni multiflori,Chinses Angelica,Semen Cassiae and the most commonly used two-drug compatibility is “Atractylodes macrocephala and Chinses Angelica” (56.52％) and “Hemp seed and Chinses Angelica” (56.52％).Conclusion Professor YYAN Zheng-hua believed that soothing the liver and regulating Qi,and regulating spleen and stomach is the basic therapeutic principle for digestive diseases.

Objective To investigate the techniques,safety,and efficacy of intraoperative holmium laser lithotripsy under cholangioscopic visualization for refractory intrahepatic stones. Methods A total of 38 patients with refractory hepatolithiasis underwent cholangioscopic holmium laser lithotripsy.The laser setting was: the wavelength of 2.1 ?m,the maximum mean output power of 100 W,the pulse peak power of up to 6 kW,and the laser fiber of 400 ?m in diameter.Results The stones were entirely removed on one session in all the 38 patients.The lithotripsy time was 3~5 min,the times of fragmentation was 3~8,and a total of 65 stones were fragmented.No bile duct injuries or biliary leakage happened.Cholangiography and B-ultrasonography at 2 postoperative months found no residual stones.Follow-up for 6 months found no recurrence.Conclusions Application of holmium laser lithotripsy under cholangioscopic visualization for the management of refractory intrahepatic stones is safe and effective.

Objective To investigate the relationship between insulin like growth factor (IGF Ⅰ)、IGF Ⅱ and IGFBP 3 and Child Pugh classification in patients with liver cirrhosis and to determine the potential clinical markers of functional hepatic reserves. Methods Forty four patients with posthepatitic cirrhosis were divided into 3 groups according to disease severity (Child Pugh Score) and 38 healthy subjects severed as controls. Serum levels of IGF Ⅰ, IGF Ⅱ and IGFBP 3 were measured in these groups by immunoradiometric assay. Results Baseline IGF Ⅰ, IGF Ⅱ and IGFBP 3 levels were significantly lower in patients with cirrhosis than in controls, and the serum concentrations of IGF Ⅰ, IGF Ⅱ and IGFBP 3 were associated with the marked changes of liver function due to Child Pugh score. They all showed a significant correlation with the degree of hepatic dysfunction and dropped markedly during the progression of liver failure. The sensitivity of IGF Ⅱ is much higher than that of IGF Ⅰ and IGFBP 3. It was found that IGF Ⅰ

Tissue engineering has evolved as a dynamic research field that encompasses multidisciplinary approaches involving cytology,material science,bioreactor engineering,and medicine.The overarching goal is to construct human tissue and organs in the laboratory for tissue regeneration or replacement.It offers a potential solution to the donor shortage in organ transplantation and to the difficulties in regenerative medicine.The basic components for tissue engineering include seed cell selection,bio-scaffold construction,and ex vivo tissue formation via a bioreactor. Over the past decade,tremendous progress has been made in bioengineering cartilage,trachea,urinary bladder,heart valve and endocrine tissues. However,there remain significant challenges in creating complex and human-sized tissues and organs for clinical use.In this article,we review the basic components and strategies for bioengineering organs. We attempt to provide an overview of current progress and challenges in developing organ-level tissue engineering and in the successful translation of bioengineered tissue and organ products into daily clinical practice.

12 ml/s was used as Group A and Qmax ≤12 ml/s as Group B. Results One week after removal of urinary catheter, dysury appeared in five cases with Qmax for (9.45?2.62) ml/s, of which two cases were with posterior urethral stricture, two with benign prostatic hyperplasia and one with weak detrusor contraction. Three months after removal of urinary catheter, six cases led to secondary dysuresia due to posterior urethral stricture and had Qmax for (6.28?3.26) ml/s, with significant difference compared with urodynamic parameters of normal group (P

Objective To study the roles of Toll-like receptor 2(TLR2)and Toll-like receptor 4(TLR4)in the progress of pulmo-nary infection with candida glabrata in experimental mice .Methods The mice were divided into three groups ,the control group (group A) ,mice infected with candida glabrata(group B)and immunosuppressive mice infected with candida glabrata(group C) .6 mice in each group were killed at 1st and 3rd day after the success in pulmonary infection with candida glabrata .The lung tissues from each group were collected for pathological analysis and PT-PCR to detect the expression level of TLR2 and TLR4 .Protein TNF alpha(TNF-α)level was measured by ELISA method .Results The pathological analysis showed the structure was normal and there was no inflammatory reaction in lungs in group A .The infiltration of inflammatory cells ,weak injuries but no conidia or hyphae in lungs were found in group B .Most of the alveolar collapse and severe damage ,part of the expansion ,a large number of in-flammatory cells infiltration ,accompanying with conidia and hyphae accumulation were observed in group C .The expression levels of TLR2 mRNA and TNF-αprotein in mice lungs at the 1st day and the 3rd day in group C were significantly higher than in group A and B(P<0 .05) ,while group B was higher than group A(P<0 .05);The expression levels of TLR2 ,TLR4 mRNA and TNF-αprotein in group C at the 3rd day was higher than the 1st day(P<0 .01) ,meanwhile ,the expression of TLR4 was higher than in group A(P<0 .05) .Conclusion TNF-αand TLR2 expression maybe involve in the infection and process of candida glabrata ,and TLR4 may play a synergistic effect .