Objective To investigate the impact of intraoperative intra-abdominal pressure increasing on digestive system.Methods A retrospective analysis of clinical data of 132 cases of colorectal cancer patients from January 2013 to June 2016 was made. Patients were divided into groups A, B and C according to the intra-abdominal pressure, 46 cases in each group. Group A: 46 patients, 10 mmHg (1 mmHg = 0.133 kPa), group B: 45 patients, 12 mmHg, group C: 41 patients, 15mmHg. Comparison of postoperative recovery time, complications, serum cytokine levels was made among the three groups.ResultsThe number of postoperative 6h nasogastric tube pulled out, bowel recovery time, ifrst lfatus or a bowel movement, tolerance semi-liquid diet, postoperative duration of diarrhea, postoperative hospitalization time among the three groups show no statistically difference (P > 0.05). The difference of acute gastric injury (AGI), chyle leakage, anastomotic fistula, surgical bleeding, intestinal paralysis, vomiting incidence of postoperative among the three groups has no statistically differences (P > 0.05). While postoperative IL-6 level and MAP were signiifcantly increased compare with preoperative level, the difference was statistically signiifcant (P < 0.05). Postoperative TNF-α show no statistical differences compare with preoperative (P > 0.05). Postoperative serum IL-6 levels in 25 patients with intestinal paralysis was (10.71 ± 4.37) ng/L, 107 cases had high serum IL-6 levels in patients did not occur intestinal paralysis was (10.66 ± 4.13) ng/L, the difference was not statistically significance (P > 0.05).Conclusion Intra-abdominal pressure increasing during minimally invasive surgical procedure has no signiifcant effect on the digestive system in colorectal cancer patients.

Humans ; In Situ Hybridization, Fluorescence ; Tissue Array Analysis ; methods

Objective To evaluate contrast-enhanced ultrasound (CEUS) for the diagnosis and follow-up of hepatocellular carcinoma ( HCC) patients after percutaneous radiofrequence ablation ( RFA). Methods The data of 46 HCC cases were reviewed in our study. Results of contrast enhanced computed tomography (CT) , ultrasound contrast agent sonography and grey ultrasound were compared with each other pre- and after percutaneous radiofrequence ablation. Results Contrast agent ultrasound imaging had the same value with CT in preoperative diagnosis of HCC, with postive likelihood ratio of 1.26 and 1.31, respectively,sensitivity of 97% and 91% and specificity of 23% and 31% respectively. For grey ultrasound the positive likelihood ratio was 0.99, sensitivity and specificity was 76% and 23% respectively. The sensitivity and specificity of contrast-enhanced ultrasound after percutaneous radiofrequence ablation was 60% and 90% respectively, and the postive likelihood ratio and negative likelihood ratio was 6. 18 and 0. 44 respectively. The difference was significant while compared with CT (P = 0. 012). All cases were followed-up from 1 to 9 months, and all were alive with recurrence found by contrast-enhanced ultrasound in 3 cases. Conclusions Contrast agent ultrasound imaging is sensitive and effective in the diagnosis of HCC and follow-up after percutaneous radiofrequence ablation.

Objective To assess the prevalence of the metabolic syndrome (MS) in Shanghai adults according to the criteria of the US National Cholesterol Education Programme Adult Treatment PanelⅢ ( NCEP ATP Ⅲ) definition. Methods Randomly selected adults were studied by means of stratified sampling. A cross-sectional study was conducted in Shanghai with a representative sample of 7 414 Chinese adults aged 35-74 years.Results Defined by the ATPⅢ criterion, the preliminary prevalence of MS was 19.81%. The prevalence of MS was 16.21% (95%CI 15.37%-17.05% ) after age was adjusted. The prevalence of MS was higher in female than in male (22.59% vs 16.64% ,P<0.01 ), and higher in urban than rural residents( 17.43% vs 17.16% ,P<0.01 ). The prevalence of MS increased with age in both male and female, especially in females aged above 55 years. There were 19.81% subjects with over 3 risk factors. The most common combination of four components in MS were central obesity, high triglyceridemia, low high-density lipoprotein-cholesterol and hypertension. Conclusions There is a high prevalence of MS in Shanghai adults. MS is increasingly becoming a noteworthy health problem. Early-diagnosis and integrated-methods of prevention and treatment of MS are mandatory.

Objective To investigate the relationship between serum nesfatin-1 and gestational diabetes mellitus (GDM). Methods The serum nesfatin-1 concentrations were measured in 45 GDM patients and 44 NGT pregnant women , as well as 47 non-pregnant healthy women served as control. Serum nesfatin-1 were measured by sandwich enzyme linked immunosorbent assay (ELISA). Meanwhile FPG、 lipoid、 FFA、 FINS、 C-tide were also measured. Result Serum nesfatin-1 level in GDM were significantly higher in GDM patients than in NGT and control (P < 0.01), while serum nesfatin-1 in NGT was significantly lower than in control (P < 0.01). Correlation analysis showed that serum nesfatin-1 was positively correlated with FINS , C-tide , HOMA-IR , triglycerides FBG and OGTT 1 hour glucose, HbA1c (P < 0.05). Conclusion Serum nesfatin-1 level in pregnant women significantly decreas, while in GDM patients significantly increase. Serum nesfatin-1 level in pregnant women is positively correlated with Insulin resistance (IR) and triglycerides level.

Objective: To study the action of Zhongjiangweiling Capsule (Fructus Amoni, Cortex cinnamoni, Rhizoma Alpiniae officinarum Radix Glycyrrhizae) on the experimental gastric ulcer. Methods: Area of ulcer, gastric juice volume, acidity and pepsin output were measured on experimental gastric ulcer models induced by the ligated pylorus and burned acetic acid and i.p Reserpine in rats. Results: Zhongjingweiling Capule showed to be able to reduce areas of ulcers, gastric juice volume, acidity and pepsin output. Conclusion: Zhongjingweiling Capsule had antiulcer effect in rats.

AIM:To discuss the related factors that affected the stability of posterior corneal surface after laser in situ keratomileusis ( LASIK) .
METHODS:About 64 patients (64 eyes) were enrolled. The correlation among the changes in posterior corneal surface 6 month after LASIK, surgery method, corneal flap thickness ( FT ) , ablation thickness ( AT ) , postoperative residual corneal stroma thickness ( RCST ) , preoperative thinnest corneal thickness ( CT ) , flap thickness/preoperative thinnest corneal thickness ( FT/CT ) , ablation thickness/preoperative thinnest corneal thickness ( AT/CT) , postoperative residual corneal stroma thickness/preoperative thinnest corneal thickness ( RCST/CT) , anterior and posterior preoperative corneal height, the difference of the forward shift in posterior corneal surface ( diff value ) of preoperative and preoperative intraocular pressure were analyzed.
RESULTS: The changes of diff value between preoperative and postoperative were related with diopter (r=0.419, P=0.014), AT (r=0.394, P=0.023), AT/CT (r=0.501, P=0.004), Diff value of preoperative (r=0.501, P=0. 004), RCST (r=-0. 385, P=0. 033) and RCST/CT (r=-0. 401, P=0. 025). The changes of height value from posterior corneal surface between preoperative and postoperative were related with diopter (r=0. 520, P=0. 002), AT (r=0.504, P=0. 003), AT/CT (r=0. 442, P=0. 013), Diff value of preoperative (r=0. 624, P=0. 000) and RCST/CT (r=-0. 394, P=0. 028).
CONCLUSION: AT, RCST, AT/CT, RCST/CT and diff value of preoperative should be the key index that predicted the stability of posterior corneal surface after LASIK,the further research will give the range of safety value.

As the integration of human parasitology and medical microbiology curriculum, pathogen biology, although constructed and developed about 20-years, didn't realize the true integration of the two disciplines. Beyond that, it was also faced with numerous difficulties such severe compression of human par-asitology class, lack of teaching materials to achieve the true meaning of integration, difficulty to cultivate teachers and so on. The organ-system based learning (OBL) curriculum model may provide new strategies for the dilemma, to be resolved through the following channels: Rearranging the curriculum and teaching materials based on the classification of pathogenic organism parasitic or invasive organs; Corresponding training in microbiology and parasitology teaching by teachers continuous realization, while highlighting the professional teaching level of highly qualified teachers in both courses.

In order to have a better understanding of the role of medical library in medical information service during the implementation of Healthy China strategy,the need of health information service of the public and medical scientific researchers in implementation of Healthy China strategy were analyzed by outlining the development history and major targets of Healthy China strategy,and how to meet the requirements of the development of Healthy China for medical library was studies,namely by improving its resources development,service patterns and staff construction according to the present situation of big data development,the challenges medical library is faced in users needs,resources,platform,space,professionals and funds,and the opportunities brought about by the expanding requirements,improving information academic level,advancing new technologies,favorable policies and environments with relevant suggestions put forward.

Aim To investigate the role of calcitonin gene related peptide(CGRP),ATP sensitive potassium channel(KATP)and spinal nerve in the protective effect of intrathecal morphine preconditioning against myocardial postischemia injury in rats.Methods Rats were established intrathecal catheter placement and myocardial ischemia reperfusion models were randomly assigned to 10 groups:control group(CON);intravenous dimethylsulfoxide group(DMSO);intravenous CGRP8-37 group(CGRP8-37,a selective CGRP receptor antagonist,3 nmol?kg~-1);intravenous glibenclamide group(GLI,a non-selective KATP channel inhibitor,0.3 mg?kg~-1);intrathecal 10 ?l of 1% lidocaine group(LID);intrathecal 3?1 ?g?kg~-1 morphine preconditioning group(MPC);intravenous 3 nmol?kg~-1 CGRP8-37+MPC group(CGRP8-37+MPC);intravenous 0.3 mg?kg~-1 GLI+MPC group(GLI+MPC);intravenous 0.3 mg?kg~-1 GLI+LID group(GLI+LID).Indicators to be observed were MAP,HR and RPP(MAP?HR),the volume of area at risk(AAR)and infarct size(IS),and the area of myocardial infarction,which was demonstrated by IS/AAR.Results Compared to CON group,the volume of IS and IS/AAR was reduced in MPC,LID,LID+MPC and GLI+LID group(P