Objective To investigate the correlation between atorvastatin inhibiting Interleukin-1β(IL-1β) expression and peroxisome proliferator activated receptor β/δ (PPARβ/δ) signal channel in myocyte of aging rat. Methods Primary culture of myocyte were got from aging rat. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GSK0660 group, whichwere treated respectively by Cell culture medium, dimethyl sulfoxide (DMSO), atorvastatin, atorvastin plus GSK0660. The expression level of IL-1βmRNA and protein was evaluated by RT-PCR and Western Blot respectively. Results (1)NO difference were found between control group and DMSO group in expression level of IL-1βmRNA and protein (P>0.05);(2)The expression level of IL-1βmRNA and protein in atorvastatin group were significantly lower than those of control group (P < 0.01); (3)Both mRNA and protein expression level of IL-1βin atorvastatin plus GSK0660 group were higher than those of atorvastatin group(P<0.05 or P<0.01), but still lower than those of control group(P<0.05). Conclusions Atorvastatin down-regulate the expression of IL-1βin aging myocytes by activating PPARβ/δsignal Channel.

Objective To explore the role of plasmids in inducing drug-resistance in Neisseria gonor-rhoeae.Methods The change of drug-susceptibility in Neisseria gonorrhoeae was compared before and after experiments of resistant plasmids conjugation,transformation and deleting.Results The drug-resistance was transferred from resistant strains to sensitive strains through the conjugation and transformation of resistant plasmids,while the drug susceptibility could be recovered through deleting such plasmids from resistant strains.Conclusion The plasmids play an important role in the development of drug-resistance in Neisseria gonorrhoeae.

Objective:To establish an animal model of oral lichen planus (OLP). Methods:Human non-erosive OLP lesion tissue was surgically transplanted into dorsal hypoderm or tongue abdomen in 10 severe combined immunodeficiency (SCID) mice, thereafter, growth and possible alterations of histopathological characteristics of the xenografts were observed for 2 weeks. Results:All the xenografts transplanted into dorsal hypoderm or tongue abdomen healed well.Partial liquefaction and subepithelial lymphocytes diffuse infiltration were observed in epithelial basement membrane in the first week after transplantation. During the subsequent week, the degenerated moiety was hardly to be seen, and the number of subepithelial lymphocytes was gradually decreased. Conclusions:The epithelia of OLP xenografts grow well in SCID mice but can not keep its histopathological features.

LAVP2. Multiple linear regression revealed duration of hypertension, grade of hypertension, age and smoking status were independent determinants for the deterioration of diastolic dysfunction. Conclusion A progressing change in regional diastolic dysfunction from right to left ventricle was found in HT patients. The grade of hypertension and the duration of HT were the major determinants on diastolic dysfunction.

To investigate the character of esophageal manometry in reflux esophagitis(RE) with or without sliding hiatus hernia(SHH). Ambulatory 24 hours esophageal pH metry and esophageal manometry were studied in 50 patients with RE, and 50 RE patients with SHH. Lower esophageal sphincter(LES) competence was significantly boorer in SHH groups as compared with RE group, esophageal sphincter length(LESL) was (1 31?0 33)cm in SHH groups and (2 10?0 86)cm in RE group, and lower esophageal sphincter pressure(LESP) was (1 17?0 53) kPa in SHH groups and (2 16?0 65) kPa in RE groups( P

To compare the diagnostic value of X ray and endoscopy in reflux esophagitis (RE) in patients with hiatus hernia(HH) and their option in clinical practice. Correlative data of X ray and endoscopic findings in 50 cases of RE with HH were analysed.The radiography showed that there was 78 57% with the reflux type of suck and 64 29% with the clearance type of passivity in HH1 group, 81 82% with the reflux type of dumpage and 90 91% with the clearance type of delay in HH2 group. X ray and endoscopic diagnoses were corresponded 60% identically. Both examination methods are suitable for diagnosis of RE with hiatus hernia, X ray is the modality of first choice. Endoscopic exemination is indicated in cases of reflux esophagitis or other complications.

BACKGROUND: Logistic analysis of multi-ordered response-variable is used to probe into from another view the interrelationship between lead content in fetus faeces and neurobehavioral development under exposure to low-level lead in uterus.OBJECTIVE: To probe into multi-factors of neurobehavioral development in neonates and the sensibility.DESIGN: The total score of neurobehavior in neonates was taken as dependent variable and 24 indexes were as independent variables, such as induced factors, lead in umbilicus blood and lead in fetus faeces in questionnaire. Logistic progressive regression of multi-ordered response-variables was used in analysis and corresponding factors were screened at level of P=0.10.SETTING: Wuhan University of Science and Technology, Laboratory Room of Occupation Disease and Epidemic disease in Tongji Medical College of Huazhong University of Science and Technology and Occupation Hospital of China First Metallurgical Construction General Company.PARTICIPANTS: Totally 103 full-month borne and healthy neonates were randomized in Department of Gynecology of one occupation hospital in Qingshan District of New-type Industry Area of Huanhan City from January to October 1999 as the objects. The relatives agreed with topic research and questionnaire investigation and they provided neonatal faeces and received neonatal tests on time.off the umbilicus and preserved in freezing in refrigerator at -4 ℃. The faeces in 24 hours after birth was collected and the lead contents of umbilicus blood and faeces were assayed with graphite furnace atomic absorpBehavioral Neurological Assessment (NBNA) was used in examination on the 3rd day after delivery. Simultaneously, the self-designed questionnaire was adopted in the investigation for parturients. The questionnaire involved other possible factors of neonatal neurobehavioral development, including dependent variables, concerning to states of family, society, environment and health that affected neurological development in neonates and lead contents in umbilicus blood and faeces. Scores of neonatal neurobehavior were taken as response variables. Finally, the corresponding factors were screened.MAIN OUTCOME MEASURES: To screen the factors of neurobehavioral development of neonates.RESULTS: Totally 103 cases entered result analysis. Six factors were selected in the model, named pregnant weeks, the month of drug administration in pregnancy, hemoglobin, emotions in pregnancy, lead level in fetus faeces and drug administration.CONCLUSION: Neonatal neurobehavioral development was related to multiple factors. Good nutrient in pregnancy, long pregnant weeks and good emotions in pregnancy benefit neurobehavioral development of neonates. Drug administration during pregnancy is disadvantageous in neonatal neurobehavioral development, especially the medication at the early phase of pregnancy. The increased lead content in neonatal faeces does not benefit neonatal neurobehavioral development.

Acupuncture Therapy ; Adult ; Catgut ; utilization ; Female ; Humans ; Lactation ; Lactation Disorders ; physiopathology ; therapy ; Postpartum Period ; physiology ; Qi ; Young Adult

Objective Queuing theory is the mathematical study of how waiting lines or queues are formed and dissipated o -ver time, which aims to work out the optimal design and optimal control of queuing systems based on the research of probability and regularity of various queuing systems .The aim of paper was to find out the maximum acceptable waiting time for outpatients in stomatol -ogy department and explore the rational allocation of dentists based on queuing theory model . Methods Questionnaires , worktime measurement and queuing theory model were applied to calculate indicators of queuing system in outpatient services of Stomatology De -partment , getting the maximum waiting time accpetable for patients and the reasonable number of dentists . Results The maximum acceptable waiting time for outpatients was 34.02 ±7.07 minutes, and it was reasonable to allocate 25 doctors in the morning and 16 doctors in the afternoon . Conclusion Applying queuing theory helps to optimize dentist number in outpatient services of Stomatology Department and provide scientific reference to improve medical efficiency .

BACKGROUND: In the past, a lot of researches used one-time lead level in umbilical cord blood at birth for investigations,however, one-time lead level in umbilical cord blood at birth can not represent lead caused cumu lative injury to neonatal nervous system during the whole period of preg nancy. Lead in meconium is mainly from digestive juice secreted by fetal alimentary tract, exfoliative epithelia from neonatal intestinal tract and am niotic fluid and sebum cutaneum swallowed by fetus, which is excreted from the very start of pregnancy to 24 hours after birth of neonates and re flects the lead deposit in neonatal intestinal tract during the whole period of pregnancy. OBJECTIVE: To explore the relationship between neurobehavioral devel opment in neonates with intrauterine exposure to lead at low level and the lead level in umbilical cord blood (CBPb) and meconium (MPb). DESIGN: Take lead levels in umbilical cord blood and meconium as neonatal intrauterine exposure indicators and scores of neonatal neurobe havioral development as effect indicators, and descriptive analysis is used to evaluate the correlativity. SETTING: Wuhan University of Science and Technology; Laboratory of Occupation Disease and Epidemiology, Tongji Medical College, Huazhong University of Science and Technology; Wuhan First Metallurgical Con struction Company Hospital for Workers and Staff. PARTICIPANTS: A total of 103 cases of full-term and healthy neonates were selected as objects of observation. The neonates were born in Depart ment of Gynecology and Obstetrics, Wuhan First Metallurgical Constrction Company Hospital, Qingshan District of New Industrial District of Wuhan from January to October 1999. Their parents were agreed to participate in the study and filled in the questionnaire, and provided neonatal meconium and performed neonatal tests on schedule. METHODS: ① Collection and assay of sample: 5 mL umbilical cord blood were collected and reserved in refrigerator at -4 ℃. Meconium with in 24 hours after birth, with dry weight between 5 to 10 g was collected, the lead levels in umbilical cord blood and meconium were assayed with the method of graphite furnace atomic absorption spectroscopy. ② Group ing: The neonates were divided into two groups with high and low-exposure to lead based on the cutoff value of CBPb of 0.483 μmol/L and MPb of 127.78 mg/kg. ③ Neonatal neurobehavioral development examination: Neonatal neurobehavioral development examination method was used for examination 3 days before delivery. Meanwhile, self-designed questionnaire was used to conduct a survey in puerperas. MAIN OUTCOME MEASURES: ① Lead levels in neonatal umbilical cord blood and meconium. ② Scores of neurobehavioral development of neonates with different lead levels of umbilical cord blood and meconium. RESULTS: All the 103 cases of neonates entered results analysis. ①There was significant difference only in scores of neonatal behavioral neurological assessment (NBNA) and biological visual and auditory orientation reaction (BVAOR)between groups with high and low-exposure to lead in umbilical cord blood (P ＜ 0.05). However, there was no rank correlativity between lead level in umbilical cord blood and scores of NBNA,non-biological auditory orientation reaction (NBAOR), non-biological visual orientation reaction (NBVOR) and biological visual and auditory orientation reaction (NBVOR). ②here was significant difference in scores of NBNA,NBAOR, NBVOR and BVAOR between groups with high and low-exposure to lead in meconium (P ＜ 0.05-0.01). The lead level in meconium clearly correlated reversely with scores of NBNA, NBVOR and BVAOR.CONCLUSION: Lead level in meconium is more sensitively related to the scores of neonatal neurobehavioral development, which could be used as indicator for lead deposit in the fetal body during the period of pregnancy.