Objective To develop a method to determine molybdenum in radix puerariae and the soil. Methods A method for determination of trace molybdenum in radix puerariae and the soil where it growth by single sweep polarography has been used. Results The linear range was 0.010-1.0 ?g/ml, r=0.999 0, the detection limit was 6.0?10-3 ?g/ml, the recovery rates were 98.0%～106.0%, RSD was 2.2%-4.8%. Conclusion This method is suitable for the determination of molybdenum in radix puerariae and the soil. The content of Mo in radix puerariae is related to that in the soil.

Injection Stauntoniae (IS ) was applied ou a part of the saphenous nerve of rat 4mm in length. With 50% IS, amplitudes of A??, A? and C components of the compound action polential were reduced and their latencics were delayed. In particular, the amplitudcs of A? and C components reduced to 47.0% ? 23.o% and 44.0?20.0% (MEAN?SD, n = 8, P

Objective To establish a method for determination of organophosphorus and carbamate pesticide residues in water by GC-MS.Methods Twelve kinds of organophosphorus and carbamate pesticide residues were determined with GC-MS with methylenechloride and petroleum ether(4∶1) as extraction solvent.Results The linear ranges of 12 kinds of pesticides were perfect in the range of 0.01-1.0?g/ml,r≥0.997,the average rates of recovery were 76.0%-111.0%,and RSD were 5.20%-9.20%,the lowest limit detection(S/N=3) of dichlorvos,chinomethionate,parathion,bromophos,carbophenothion,fenthion,isoprocarb,pirimicarb,aminocarb,thiobencarb,pendimethalin,malathion was 0.009,0.003,0.008,0.001,0.003,0.003,0.0025,0.009,0.005,0.010,0.010,0.006 ?g/ml respectively.Conclusion This method is simple,rapid,sensitive and can be applied to the simultaneous determination of 12 kinds of organophosphorus and carbamate pesticides in the water.

Objective To analyze the main clinical manifestations of intestinal lipoma and discuss the treatment of intestinal lipoma.Methods The clinical data of 19 patients with intestinal lipoma treated in our hospital in recent 15 years were analyzed retrospectively.CT,ultrasonography,and colonoscopy were used in diagnosis.Local intestinal resection by laparoscopy and laparotomy were employde.Results Intestinal lipoma presented with a variety of symptoms including intestinal bleeding,abdominal pain,anemia,intussusception,and bowel obstruction.In 17 of the intestinal lipoma patients,the main clinical manifestations were change of bowel habits,abdominal pain and bloody stool;13 of them had intestinal intussusception or obstruction,and two patients were asymptomatic.Sixteen patients underwent colonoscopy,and seven of them were correctly diagnosed.Seventeen patients received surgical resection and recovered.The diameter of mass was 3-6.5 cm.The pathologic exam showed submucosal lipoma in 12 cases,two cases showed ulcer formation with erosion,2 cases had intermural lipoma and one case had atypical lipoma with potential malignant transformation.Two asymptomatic patients diagnosed by colonoscopic biopsy werre only followed up at regular intervals.Conclusions Imaging diagnosis and colonoscopy are helpful in the preoperative diagnosis of intestinal lipoma.The method of treatment is based on the size of the tumor and presence or absence of pedicle.Endoscopic electrosection is often used for tumor mass less than 3 cm in diameter,otherwise local intestinal resection is the treatment of chocie.

Nineteen cases of cardiac myxoma were studied immunohistochemically in order to elucidate the histogenesis of the tumor.It was found that the blood vessels and the stromal cells of all the tumors were positive to vimentin.the matured endothelial cells of blood vessels were positive to UEA.the stromal cells and the smooth muscle cells of blood vessels of a part of the tumors were positive or weak-positive to desmin or myosin.a part of the tumor cells of several myxomas were weak-positive or positive to S-100 protein,and the epithelial markers of AE1 and AE3 were negative in all the cardiac myxomas.Our study revealed that there were various kinds of mesenchymal cells in cardiac myxomas and they might originate from multipotential primeval mesenchymal tissues.The difference of im-munohistochemical reactions of tumor cells reflects the difference of the maturity and differentiation of the tumor tissues.The histopathology and histochemistry of cardiac myxoma were also observed and analyzed.

Adolescent ; Adult ; Arsenic Poisoning ; drug therapy ; Child ; Chronic Disease ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy

Objective To explore the incidence and predictive factors of anemia induced by chemothera-py in early breast cancer patients. Methods 400 early breast cancer patients treated by taxane-based regimens from 2009 to 2011 in our hospital were analyzed to obtain the incidence of anemia. Univariate analysis and multivariate logistic regression analysis were used to search for risk factors linked to the occurrence of anemia. Results Incidence of anemia was 72.2% in early breast cancer patients undergoing chemotherapy. The occur-rence of anemia was related to 5 risk factors: chemotherapy regiments, Hb at baseline < 135.0 g/L, age > 60 years old, BMI ≤ 25 kg/m2 and HBV antigen positive. Conclusion The anemia incidence during chemothera-py is high in early breast cancer patients. Such factors,as chemotherapy regiments, Hb at baseline, age, BMI and HBV antigen, should be taken into account in identifying high risk patients and prevent anemia.

Objective To evaluate the performance of fast Treponema pallidum(TP) detection in voluntary blood donors and optimize the strategy for pre-donation screening.Methods Before blood donation,the gold standard TP test strip was used to make a fast detection.After blood donation,the TP-ELISA was used to test the blood.Then,analyze the donors′ anti-TP positive rate,times and intervals of donating,false positive and negative of TP fast detection.Results From 2014 to 2015,among 73 990 donors who were tested by using fast TP detection,0.71% of them(529 donors) were positive.Among the positive donors,89.2% of them(472 donors) were first-time blood donors.35 donors′ donating intervals were more than 3 years,who accounted for 61.4% of the donors who had donated for more than once.The numbers of the false positive obtained from fast TP detection were 5 and the false negative was 15.By applying the fast TP detection before blood donation,the rate of anti-TP positive had been declined from 0.71% to 0.17%.Conclusion The rejection rate of TP positive can be significantly reduced by using fast TP detection before blood donation.The fast TP detection can be used to optimize the pre-donation screening and promote blood donation service efficiency and level,while donating times and intervals of the blood donors were also considered.

Objective To study the protective effect of bionics-pulsed electromagnetic field (BEMF) on the biomechanical property of the bones of Wistar rats. Methods Thirty-two 3-month-old female Wistar rats were divided into 4 groups (n=8 in each group). The 8 rats of group A (the control) were treated normally and received injections of saline in the same amount of drugs for the rats of other 3 groups. The rats of group B received injections of desamethasone (1 mg/kg, 2 intramuscular injections/week). The rats of groups C and D received injections of desamethasone in the same procedure as that of group B. In addition, the rats of group C received daily subcutaneous injections of calcitonin in the dosage of 2 IU/kg and those of group D were irradiated with BEMF once daily. The results of the 3 kinds of therapeutic procedures were observed 8 weeks later. Results After the irradiation of BEMF, the biomechanical parameters of the lumbar vertebrae and the femur were far higher in the rats of group D than in those of group B. Conclusion BEMF irradiation is effective to prevent the decrease of biomechanical properties of the bones of the rats.

Objective To investigate the relationship between androgen receptor CAG-gene polymorphism and androgen in male with salt sensitive hypertension.Methods Through the oral saline loading test and furosemide volume method male hypertension group were divided into salt-sensitive (SS group) and salt-insensitive (SR group).The samples from 161 males were selected in the study,including salt sensitive hypertension patients (SS group,61/161),salt-insensitive hypertension patients (SR group,40/161) and age-matched healthy samples (control group,60/161).All samples were sequenced with an analysis method (CAG) n repeated polymorphism,and determinated of total testosterone (TT) and free testosterone (FT) level in serum by electrochemiluminescence immunoassay.Results The number of CAG repeats was 14～34,average 22.4± 2.7.The CAG repeats of SS,SR and control group were 23.5±3.75,22.3±3.17 and 21.8±2.95,respectively.There were significant differences among the three groups (t=2.627～ 3.257,all P＜0.05).The level of TT and FT in SS and SR group were decreased compared with that of control group.At the same time,the level of SS group was lower,and there were significant differences among the three groups (t=2.524～ 3.826,all P＜0.05).Conclusion The androgen receptor gene repeat length and androgen levels are associated with male hypertension,especially salt-sensitive hypertension.Long (CAG) n repeat polymorphism maybe a genetic factor in the pathogenesis of hypertension.Plasma androgen levels may be used as a predictor of male salt sensitive hypertension.