Objective To detect the relaxation property of mesoporous silicananocapsules loaded liposomes (MSN-LIPO), and to explore their functions of enhancing MRI T2 weighted imaging. Methods In vitro, MSN-LIPO with different ironconcentrations were scanned by MRI system to detect their relaxation properties. In vivo, six BALB/c nude mouse models with subcutaneous malignant glioma were established and randomly divided into two groups, which were injected with the same concentration of MSN-LIPO intratumorally and intravenously, respectively. The mice in the two groups were scanned by MRI before and after injection, and the image characteristicswere studied and the signal intensity of tumors was statistically analyzed. Results In vitro MRI imaging showed that the signal intensities of the images were gradually decreased with the increase of iron concentrations in MSN-LIPO, and 1/T2 had a good linear relationship with the concentration within a certain range, with the relaxation rate (r2) being 413.7 mmoL-1 s-1. MRI imaging of BALB/ c nude mouse models with malignant glioma showed that the signal intensity of the tumor after intratumor injection of MSN-LIPO was significantly lower than that before intra-tumor injection (15.34±1.24 vs 211.44 ± 5.34, t=36.38, P < 05), and the signal intensity of the tumor after intravenous injection of MSN-LIPO was significantly lower than that before intravenous injection (179.00 ± 4.35 vs 235.99 ± 5.17, t=14.34, P <0.05). Conclusion MSN-LIPO has good magnetic relaxation property and good MRI T2 weighted contrast enhancement function in vivo, suggesting that it can be used as a MRI T2 contrast agent.

A 23-year-old man in remission from acute myeloblastic leukemia after allogeneic peripheral blood stem cell transplantation developed peripheral neuropathy presenting as sciatic and peroneal nerve deficits. Electrophysiological tests localized the lesions to the left sciatic and common peroneal nerve. Magnetic resonance imaging revealed nerve thickening and enhancement, while a positron emission tomography-computed tomography scan demonstrated increased fluorodeoxyglucose uptake tracking along the nerve, suggesting peripheral nerve infiltration. This report demonstrates an unusual presentation of acute leukemia relapse presenting as focal neuropathy

Objective To prepare and characterize a thermo-sensitive liposomes co-loaded with IR-780 and doxorubicin (D0X). Methods Membrane hydration method and ammonium sulfate gradient method were used to prepare IR-780/D0X thermo-sensitive liposomes (DITSL). The particle size, zeta potential and polydispersity coefficient (PDI) of liposomes were measured by Malvern laser particle size analyzer, and the drug release characteristic of DITSL induced by laser was also detected. Results DITSL loading both IR-780 and D0X was successfully prepared. The encapsulation efficiency of IR-780 and D0X was (94.47 ± 8.57) % and (92.52 ± 7.61)%, respectively; the average particle size was (138.98 ± 8.74) nm, with slight negative potential; the PDI was 0.32 ± 0.02. The drug release rate of DITSL was about 80.1% after laser radiation (0. 8 W/cm2, 5 min) and the highest temperature of DITSL was 54.2°C. Conclusion The prepared DITSL has high drug encapsulation efficiency, appropriate particle size, high photo-thermal conversion efficiency, good temperature sensitivity and laser-induced thermal drug release property, which lays a foundation study for the combination treatment of tumors with photo-thermal therapy and chemotherapy.

To make clear of the absorbed components of Tianzhusan (TZS) and its possible mechanism in preventing vascular dementia (VD), the rats' models of VD were prepared by a permanent ligation of the bilateral common carotid arteries. After 60 days, rats were administrated with TZS for 0.1 g x kg(-1), and the volume is 0.02 mL x g(-1). After 3 days, the medicated serum was prepared and detected by UPLC, and then we predicted the possible chemical structure of the absorbed components of TZS. According to the absorbed components, the potential targets of TZS were found by ligand profiling of Discovery Studio 3.5. All of these target genes were submitted to DAVID onine for gene set enrichment analysis (GSEA). The 5 absorbed components of TZS have been predicted, and four of them have been identified as parishin B, parishin C, parishin, pennogenin-3-O-alpha-L-rhamnopyranosy-(1-->2)-beta-D-glucoside. Through reverse finding targets, we got 861 pharmacophore models and 9 pathways from KEGG, BIOCARTA after document verification. These results showed that the efficacy mechanism of TZS on VD perhaps were be related with these absorbed components and pathways. If the traditional herbs could be proved effective by efficacy tests, the serum pharmacochemistry, computer-aided drug design, system biology and other technologies can be used in the next experiments, which will be beneficial to fast discovery of material basis and mechanisms of traditional medicine coming form ethnic minorities.
Animals ; Dementia, Vascular ; prevention & control ; Drug Discovery ; Drugs, Chinese Herbal ; therapeutic use ; Gastrodia ; chemistry ; Male ; Medicine, Chinese Traditional ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Mass, Electrospray Ionization ; Trillium ; chemistry

Neoadjuvant therapy has been widely applied in the treatment of rectal cancer, which can shrink tumor size, lower tumor staging and improve the prognosis. It has been the standard preoperative treatment for patients with locally advanced rectal cancer. The efficacy of neoadjuvant therapy for rectal cancer patients varies between individuals, and the results of tumor regression are obviously different. Some patients with good tumor regression even achieve pathological complete response (pCR). Tumor regression is of great significance for the selection of surgical regimes and the determination of distal resection margin. However, few studies focus on tumor regression patterns. Controversies on the safe distance of distal resection margin after neoadjuvant treatment still exist. Therefore, based on the current research progress, this review summarized the main tumor regression patterns after neoadjuvant therapy for rectal cancer, and classified them into three types: tumor shrinkage, tumor fragmentation, and mucin pool formation. And macroscopic regression and microscopic regression of tumors were compared to describe the phenomenon of non-synchronous regression. Then, the safety of non-surgical treatment for patients with clinical complete response (cCR) was analyzed to elaborate the necessity of surgical treatment. Finally, the review studied the safe surgical resection range to explore the safe distance of distal resection margin.
Humans ; Neoadjuvant Therapy/methods* ; Margins of Excision ; Treatment Outcome ; Rectal Neoplasms/pathology* ; Rectum/pathology* ; Neoplasm Staging ; Retrospective Studies