The application of human embryonic stem cell(hES cell) contributes a lot to the disease treatment,and shows a great potentiality especially in organ transplantation and tissue engineering.Although hES cell therapy has not reached clinical application,some newly achieved results are encouraging.This review mainly presents results recently published in applying hES cell for disease treatment.

Adolescent ; Adult ; Age Determination by Skeleton ; methods ; Child ; Child, Preschool ; China ; Diagnosis, Computer-Assisted ; methods ; Female ; Humans ; Male

Adult ; Female ; Humans ; Intestinal Obstruction ; etiology ; therapy ; Postoperative Complications ; etiology ; therapy ; Rectal Neoplasms ; radiotherapy ; surgery

FOXM1 is a member of the FOX family of transcription factors and has been confirmed to participate in cell prolifera-tion and cell cycle progress. FOXM1 has been reported to play an important role in more than 20 types of human neoplasms, and its up-regulation promotes tumor cell proliferation, invasion, metastasis, chemotherapy drug resistance, and resistance to apoptosis. In recent years, various studies have shown that FOXM1 also plays an important role in tumor metabolism. The metabolism of cancer cells deter-mines the growth and survival of cancer, and metabolic alteration is a key feature of tumor cells. The connection between metabolic ab-normalities and tumorigenesis has been reported. The O-linkedβ-N-acetylglucosamine transferase regulates the expression of FOXM1, which helps tumor cells survive without damage caused by oxidative stress. This review aims to summarize the known relationships of FOXM1 and tumor cell metabolism, as well as its role in the potential pathways of tumor metabolism.

Objective To study the efficacy, safety and compliance of insulin human analog insulin aspart (NovoRapid) and insulin zinc protamine analog (Basulin) in the application on type 2 diabetes mellitus(T2DM)pafients during perioperative period. Methods Sixty T2DM patients were divided into 3 groups (n=20). Group A was treated with NovoRapid+Basulin, group B was treated with insulin human (Humulin R + Humulin N) and group C was treated with insulin pump (Humulin and NovoRapid). Blood glucose was detected in all of the patients before the meal, 2 hours after meal, before the sleep and during the operation. Results Blood glucose could all achieve the control level with the three methods of treatment. The rates of hypoglycemia in the three groups were 10%, 30% and 5% respectively, and the rates of patient satisfaction were 95%, 70% and 100% respectively. Conclusions Insulin human analog could control blood glucose effectively and safely, the compliance is also high. It is feasible of applying insulin human analog in the blood glucose control during perioperative period.

Adolescent ; Humans ; Male ; Werner Syndrome

OBJECTIVE: To develop a sensitive and specific reverse-phase high-performance liquid chromatography (RP-HPLC) method to determine propofol in follicular fluid. METHODS: Thymol was added into follicular fluid samples as the internal standard, and the samples were then precipitated with methanol. After centrifugation at 20000 r·min-1 for 15 min, 20 μL of supernatant was directly injected into an Agilent Extend-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was composed of methanol-water (77:23). Analysis was run at a flow rate of 1.0 mL ·min-1. Fluorescence detection was applied with excitation and emission wavelengths of 276 and 310 nm, respectively. RESULTS: The calibration curves had good linearity in the range of 0.108-5.4 mg·L-1. The linear regression equation was A=0.2074ρ-0.0012(r=0.9993). The limits of detection and quantity proved to be 54 and 108 μg·L-1, respectively. The method recoveries for low, intermediate and high concentrations were 117.9%, 101.7% and 99.4%, respectively. The RSDs of inter-day assay were 4.10%, 1.28% and 0.20%(n=5), and intra-day assay were 4.34%, 2.33% and 0.61%(n=5), respectively. CONCLUSION: The method is accurate and convenient. It can be used to determine propofol for studying its clinical pharmacokinetics.

Objective To evaluate the efficacy of pneumocystoscopy for congenital malformation of the vesicoureteral junction. Methods A total of 18 children (male 11 and female 7, aged from 3 months to 7 years with a mean of 3.6 years) with congenital malformation of the vesicoureteral junction (22 ureters) were enrolled in this study. Among the patients, 9 had vesicoureteral reflux (4 of them had bilateral reflux), 2 had bladder diverticulum complicated with urethral stenosis, and 7 had urethral stenosis and ipsilateral hydronephrosis. After the bladder was insufflated with CO2 through a Foley catheter (16 mm Hg), three trocars were inserted into the bladder, and the bladder was fixed on the abdominal wall using silk thread. By pneumocystoscopy, the Cohen procedure was done through the trocars. A Foley catheter was indwelled at the end of the procedure. Results The procedure was completed in all but one patient, who was converted to open surgery because the contraction of the urethra. The mean operation time was 130 min (110 to 145 min) for unilateral cases (13), and 242 min (225 to 250 min) for bilateral cases. The patients were discharged form hospital 9 to 12 days after the operation, and were followed up fro 6 months to 5 years with a mean of 3.8 years. During this period, MUC showed no ureteral reflux. One of the cases developed mild infection of the urethra, and was cured by circumcision. Conclusion Pneumocystoscopy is effective for congenital malformation of the vesicoureteral junction.

Objective To evaluate the application of fluorescent multiplex amplification of short tandem repeat(STR)for monitoring survival of engraftment after bone marrow transplantation(BMT).Methods Three STR loci named D12S391,D18S865 and D20S161 in 56 cases were detected by fluorescent multiplex amplification.PCR products were separated and typed by DNA Sequencer.Results The genotypes of STR in 52 recipient after bone marrow transplantation were completely identical with those of the donors.In another 4 cases the evidences of mixed chimerism were observed.Conclusion The system of fluorescent multiplex amplification of STRs exhibited high capacity of discrimination and low cost.Its application in the detection of STR after BMT is reliable,sensitive and simple.Combined with the clinical manifestation it can be used to evaluate the effect of BMT.