Cumene Hydroperoxide(CHP)-induced mouse mitochondrial lipid peroxides (LPO) are significantly higher than those of the normal mitochondria ( P

Male mice were subjected to 6 Gy total body irradiation,20% TBSA full thickness burns,or both the injuries respectively,and then lipid peroxides (LPO),vitamin E,sulfhydryl groups,respiratory control ratio (RCR),ADP/O ratio,and cytochrome oxidase activity of hepatic mitochondria were determined in the animals in the first 9 postinjury days.It was found that.1.LPO level increased in the early postinjury stage in the combined radiation-burn injury group.2.Vitamin E level decreased significantly in both the groups with either only irradiation or burns.3.The sulfhydryl groups showed a tendency to increase in all the 3 groups.4.The activity of cytochrome oxidase increased signficantly on the 7th postinjury day in the combined injury group.5.RCR and ADP/O ratios decreased more significantly in the combined injury group than in either the single injury group.These facts suggest that the suppression of the respiratory function of hepatic mitochondria results from the damage of mitochondrial membranes due to iipid peroxidation.

The fatty acid content of liver mitochondria was quantitatively determinde with gas chromatography in rats after they were inflicted with 15% TBSA full thickness burns or after the liver mitochondria were incubated with lipids extracted from eschar tissue(D1)or from normal skin of rats(D'1).It was found that after burn injury,the content of unsaturated fatty acid of liver mitochondria decreased and that of saturated fatty acid increased significantly.Both Dl and D'l decreased the content of unsaturated fatty acid and the decrease would be more significant when the concentration of eschar lipids or skin lipids was increased.D1 reduced the unsaturated fatty acid content more significant than D'1 when the two were in same concentration.The possible mechanism of the decrease of unsaturated fatty acid in the liver mitochondria induced with burn injury,eschar lipids or normal skin lipids was discussed briefly.

Lipid extract of burn eschar (D_1) added to isolated rat liver mitochondrialcaused depression of respiratory control ratio (RCR), ADP/O ratio, rate of ATP produc-tion, respiration rate in state 3 but it stimulated the respiration rate in state 4. Pretreat-ment with vitamin E significantly prevented the inhibition of mitochondrial respiratoryfunction caused by D_1. The addition of vitamin E after that of D_1 had no protective ef-fect. Heat-treatment of D_1 markedly reduced its inhibitory effect on mitochondrial respira-tory function. Cumene hydroperoxide (CHP) markedly depressed mitochondrial respiration.Vitamin E caused a significant increase in RCR, which was inhibited by CHP at lowerconcentrations. Vitamin E reduced MDA formation considerably in normal mitochondriaand in those mitochondria incubated with D_1, heated D_1 or CHP. These findings indicatedthat D_1 -and CHP-induced depression of mitochondrial respiratory function was probablydue to lipid peroxidation damage which was proved by the protective effct of the pota-tive antioxidant vitamin E.

Objective To discusse the acupuncture treatment of blood-stasis syndrome of liver meridian based on the ancient medical records. Methods Medical records on treatment of blood stasis syndrome of liver meridian were searched in Chinese Medical Classics. Frequency analysis and comprehending analysis were used to study the treatment strategies and methods. Results Among 231 medical records, single acupoint treatment accounts for 47.28%, followed by multi-acupoints treatment accounting for 32.16%. The most used single points are Taichong (15.96%) and Ganshu (11.70%). Yanglingquan point and Zusanli point are used mostly in two-combined acupoints treatment, while Sanyinjiao is mainly used in multi-acupoints treatment. And the liver channel of foot-Jueyin is mainly used in the meridians, accounting for 24%, followed by the bladder meridian of foot-Taiyin. Conclusions The acupuncture treatment is very effective, while the single acupoint and two-combined acupoints are easily used. It can improve the clinical effects.

Objective To assess the feasibility of combined laparoscopic multi-organ surgery in clinical appliation. Methods 48 combined laparoscopic multi-organ surgeries performed from August 2000 to November 2001 were analyzed. Results All laparoscopic procedures were successfully completed.No one needed to be converted to laparotomy and had postoperative complications.Postoperative stay was similar to that of patients under laparoscopic mono-organ surgery in the same time. Conclusions Combined laparoscopic multi-organ surgery can be performed conveniently.It offers significant benefits to patients such as decreased postoperative pain,reduced trauma to the abdominal wall and cost-saving.

Objective To compare the application effect of the Sandwich teaching method and case teaching method in undergraduate nursing students in clinical nursing teaching.Methods 114 undergraduate nursing students were divided into observation group and control group by random number table,and each group had 57 students.The control group used case teaching method,which the teacher used case to teach,while the observation group used the Sandwich method,including teacher guides,group discussions,cross discussion,group report,teacher's comments,case analysis,etc.Results The score of theory test in observation group was 86.14±7.24,which was significantly higher than 77.46±6.97 of the control group (t=6.515,P ＜ 0.01);Students of observation group thinking yes in the nine survey items was 85.96％ to 100.00％.Conclusions The effect of Sandwich teaching method is better than case teaching method,and has high satisfaction of nursing students.

Objective:To study parenting style of difficultly educated children and the state of their mental health.Methods:Randomly select 120 difficultly educated children from a Training Camp in Changsha and used SCL-90 and EMBU to rate their parenting style and mental health.Results:The mental health condition of difficultly educated children was significantly worse than the norms,but the normal middle school students parental warmth was higher than the difficutly educated students.Difficultly educated children scored significantly higher in father's firm control,punishment,over interference,rejection and denial and maternal rejection and denial,firm control and punishment,but significantly lower in parental warmth.Conclusion:Difficultly educated children had got more mental problems,and their parents treat them with more negative parenting style and less positive parenting style.

[Objective] To explore effect of momordin on reversion of multidrug resistance(MDR) in K562/A02 cells,and expression and function of P-glycoprotein(P-gp).[Methods] IC50 was detected by CCK-8,and expression of P-gp and retention of ADM in K562/A02 cells were detected by flow cytometry(FCM).[Results] Momordin can improve sensitivity of K562/A02 cells to many kinds of chemotherapeutical drugs,result in decrease of expression of P-gp,and raise concentration of ADM within cells.[Conclusion] Momordin can partly reverse drug resistance of K562/A02 cells,the mechanism of reversion is related with down regulation of expression of P-gp.

Objective To evaluate the relationship between the number of copies of genes and congenital diaphragmatic hernia by the detection of multiple loci in infants with congenital diaphragmatic hernia. Methods Multiple loci were analyzed by Microarray analysis of Affymetrix Cytoscan 750 k in 11 neonates with congenital diaphragmatic hernia, in whom 1 case was twins,and his fraternal twins were diagnosed of fetuse intestinal dilatation. Results A homozygous deletion (8 p11.22 arr[hg19]) was found in one neonate with congenital diaphragmatic hernia, and was eventually confirmed that the depolymerization of the biotin and metalloprotease (ADAM) 3A genes lead to homozygous deletion of the 1～15 exon. Conclusion The alteration of ADAM3A copy number may be the cause of congenital diaphragmatic hernia.