Objective To discuss clinical significance of the neutrophil alkaline phosphatase(NAP) activity in polycythemia pa‐tients .Methods 35 cases of patients with secondary polycythemia(secondary group) and 22 patients with polycythemia vera (poly‐cythemia vera group) were enrolled in the study ,whose peripheral blood smears were stained by using chemical method and the pos‐itive rate and scores of NAP were assessed .In addition to that ,30 cases of healthy people who had underwent physical examination were recruited as control group .Results NAP positive rate and scores of the polycythemia vera group were significantly higher than those of secondary and control group(P<0 .05) ,while no statistical difference was observed between secondary and control group(P>0 .05) .Conclusion NAP score is a good methord to diagnose polycythemia ,which could facilitate rapid ,accurate diagno‐sis and be used as auxiliary indicators in the differential diagnosis of polycythemia .

Objective To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 (STYK1) on the proliferation,apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15.Methods The expression of STYK1 protein was downregulated by RNA interference technique in HCT-15 cells.Then,the STYK1 protein expression was detected by Western blotting.CCK-8 assay was used to measure the effect of STYK1 down-regulation on the proliferation of the cells,flow cytometry was employed to detect the cell apoptosis,and Transwell chamber assay was adopted for cell invasion ability.The expression levels of p-ERK1/2 and p-AKT were detected by Western blotting.Results Compared with the siRNA NC group,STYK1 down-regulation significantly inhibited the proliferation (P ＜ 0.01) and invasion (P ＜ 0.01),induced cell apoptosis (P ＜ 0.01),and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA (P ＜0.01).Conclusion STYK1 down-regulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells,which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.

[Objective]This paper mainly introduced Professor YE Jin 's experience in treating girls with precocious puberty from liver syndrome differentiation and characteristics of prescription medication.[Methods] On account of three main symptoms of girls with precocious puberty at first visit, including thelarche, leukorrhea and menses, we explore the relation between liver and the causes of this disease from the viscera-state doctrine as well as the meridian theory. Then introduce Professor YE Jin's methods and empirical medication in treating girls with precocious puberty based on liver. In order to analyse her experience, a clinical case was reported. [Results]Professor YE holds that the pathogenesis of girl 's precociouss puberty closely related to liver. The deficiency of liver-yin and kidney-yin is the basic pathogenesis of precocious puberty, which is often accompanied with liver qi depression or even liver qi depression transforming into fire. On the basis of nourishing yin and purging fire, Professor YE emphasizes treating precocious puberty from liver. In practical terms, flexibly applying the ideas of softening, soothing and clearing liver can effectively improve the clinical symptoms. She makes the selection and compatibility of Chinese medicine comforming to the characteristics of children 's physiology and pathology and values the protection of vital energy. The reported case specifically demonstrates treating a girl 's thelarche from liver syndrome differentiation as well as the empiric therapy. [Conclusion]Professor YE Jin's experience in treating girls with precocious puberty is well worth learning for its significant curative effect.

Objective:To observe the effect of prolonging life and inhibiting tumor growth of Xiaozhensan on H22 cells-bearing mice and study the mechanism of action. Methods:①H22 cells-bearing mice models were made by subcutaneous injection and were divided into six groups:model group,low,middle and high dosage groups of Xiaozhensan,5-FU group,5-FU plus Xiaozhensan middle dosage group. After 10 days' treatment,the mice were killed and the anti-tumor rates were calculated,to detect the content of IL-2 and observe pathological sections of tumor and expression of Bcl-2 by immunohistochemical method. ②H22 cells-bearing ascites mice models were divided as the above. The living days of the mice were recorded,the prolongation of life span were calculated. Results:Xiaozhensan had the function of inhibiting the growth of tumor,and there was the dependability between quantity and effect. In pathomorphology,the sections of Xiaozhensan groups showed nuclear shrinkage,necrosis,hemorrhage,inflammatory cell infiltration on the periphery,etc. Xiaozhensan could increase the content of IL-2 and inhibit the expression of Bcl-2. Xiaozhensan combined with 5-FU can prolong the life span of the mice bearing ascites tumor. Conclusion:Xiaozhensan had the function of inhibiting the growth of tumor. The mechanism of action may be concerned with these aspects:enhancing the cellullar immunologic response and strengthening the ability to resist tumor,inducing apoptosis and weakening reproductive activity of tumor,etc. Middle dosage group of Xiao Zhen San combined with anticarcinogenic western medicin can prolong the life span of the mice bearing ascites tumor. This study provided dependable experimental documents for clinical application of Xiaozhensan,and had some guiding significance in clinic.

[abstract] Objective To study the health education on diabetes nurse diabetes management mode at the core of application in the patients with follow-up effect. Methods Our hospital endocrinology in October 2015,participate in blood sugar management company research project(namely,doctors,nurses and patients ofthree Eonline and offline (O2O) integration management system).The project is the specialist education nurses,doctors and patients of organic whole,to form a closed-loop management.Use of the project with mobile tablet (PAD),SMS platform on the first base into a group of patients with type 2 diabetes insulin for 5 minutes on education and baseline data collection,and in the next 15 weeks of diabetes health education nurses via text message and phone tracking in the form of blood sugar and insulin dose data,at the same time for more than three months of education,guidance and management,to help patients with initial foundation of insulin self management knowledge and skills,promote the blood glucose control is at target. Results In 12 weeks of the completion of this project and follow-up,425 patients of FPG success rate is 82.6%. Conclusion Nurse diabetes health education as the core of diabetes management model is feasible in patients with type 2 diabetes, the effect is good,that greatly arouse the subjective initiative of patients;Improve the patients′self-management ability,improving according to the medical behavior;Improved treatment adherence to standard prompt patients FPG.

A girl who aged eight years and seven months presented with prunosus patches on the right buttock for 8 years,gradual unilateral enlargement of the right lower limb for more than 7 years,and multiple vegetations for 1 year.Dermatological examination showed nevus flammeus and multiple malodorous vegetations over the right lower limb with high skin temperature.The right lower limb was thicker and longer than the left lower limb.X-ray examination,magnetic resonance imaging and Doppler ultrasound examination revealed high-flow vascular malformations.Pathological examination of the vegetations showed vascular proliferation,fibroblast proliferation and erythrocyte extravasation.She was diagnosed as Parkes-Weber syndrome accompanied by pseudo-Kaposi's sarcoma.

OBJECTIVE:To observe the protective effect of the traditional Chinese medicine Yiqimingmu oral solution on photochemical damage of retina in rats. METHODS:SD rats were divided into four groups randomly:negative control group, model group, low dose Yiqimingmu oral solution group and high dose Yiqimingmu oral solution group. All the groups except the control one were continually exposed to Lux (1 900?106.9) green fluorescent light for 24 hours to establish retinal photochemical damage model. The low dose and high dose groups were administered intragastrically with Yiqimingmu oral solution (15mL?kg-1?d-1 and 30mL?kg-1?d-1) 7 days before light expose. Normal saline solution was administered intragastrically in control and model group. At 6 hours and 6, 14 days after light expose, the content of malandialdehyde (MDA) and activity of SOD in rats' retina were measured. The histopathologic changes were observed by transmission electron microscopy and light microscopy. RESULTS:At 6 and 14 days after light expose, the activity of SOD in Yiqimingmu solution high dose group were higher than in the model control group(P

BACKGROUND: Primary progressive aphasia syndrome(PPA) is an unusual type of dementia, and the relevant reports are very rare in China.OBJECTIVE: To describe a Chinese PPA patient with non-fluent aphasia as the main clinical characteristic.DESIGN: An observed trial and a case analysis based on patients.SETTING: Department of Neurology in a hospital of a university and a general hospital of a military area command of Chinese PLA.PARTICIPANT: The patient was a 64-year-old and right-handed man, who hospitalizated in First Clinical Medical College, Harbin Medical University in April 2002.METHODS: Neuropsychological assessment, head structural computed tomograph and magnetic resonance imaging and functional single photon emission computed tomography(SPECT) imaging were used in this patient.ing results.RESULTS: Neuropsychological testing revealed predominantly non-fluent aphasia, anomia was the earliest symptom. CT scan and MRI revealed atrophy of left temporal convexities and enlarged sylvian fissures. SPECT exhibited hypoperfusion in the left temporal, frontal, and partially parietal areas.CONCLUSION: The clinical symptoms, the neuropsychological and neuroimaging findings fit the diagnosis of PPA.

Background Angle closure is the pathological basis of primary angle-closure disease (PACD).Understanding different types of angle closure mechanisms is significant for evaluation of risk factors,prevention,diagnosis and treatment of PACD.Ultrasound biomicroscopy (UBM) is commonly used for investigating the types of angle closure mechanisms in clinical works.However,UBM is a contact examination,which limits its application.Anterior segment optical coherence tomography (AS-OCT) is a non-contact examination of anterior segment imaging,through which qualitative and quantitative evaluations of the anterior chamber angle can be made.Objective This study was to investigate the reproducibility of mechanism categories of angle closure based on AS-OCT images and UBM images.Methods Cross-sectional study was performed.PACD outpatients from September to October in 2013 in Beijing Tongren Hospital were enrolled and received both UBM and AS-OCT examinations.UBM images were obtained in superior,inferior,nasal and temporal quadrants of the anterior chamber.AS-OCT images were obtained in the anterior segment quadrant mode at 0-180,45-225,90-270,and 135-315 degree meridians.UBM and ASOCT images were categorized into three dominant angle closure mechanisms:pupil block,anterior located ciliary body and thick peripheral iris roll,separately.And the same mechanism of at least two UBM/AS-OCT images of one eye was defined as the angle closure mechanism of this eye.If both eyes of one PACD patient were eligible for inclusion,the right eye was selected for analysis.The reproducibility of two examinations was evaluated by Kappa coefficients.Results Finally 40 PACD patients (40 eyes,27 right eyes and 13 left eyes) were enrolled for analysis.Based on UBM images,12 eyes (30.0％) were classified as pupil block,23 eyes (57.5％) as anterior located ciliary body and 5 eyes (12.5％) as thick peripheral iris roll.Based on AS-OCT,12 eyes (30.0％) were classified as pupil block,21 eyes (52.5％) as anterior located ciliary body and 7 eyes (17.5％) as thick peripheral iris roll.The Kappa coefficient for the classification of angle closure mechanisms was 0.870.Conclusions Criterion of angle closure mechanism categories based on AS-OCT images was established.Mechanism categories obtained from AS-OCT and UBM images showed a good reproducibility.

Objectives To search for the relationship between HP and lesions of gastric mucosa of children.Methods Four hundred and fifty-eight bioptic specimens-from eastrie mueosa in children were strained with hematoxylin-eosin(HE)and Warthin-Starry for light microscopic study.Results HP was detected in 152 specimens. HP deteetable rate-in specimens with normal mucosa was 1. 1%, in compari-on with 42.3% in those with chronic superficial gastritis and 14. 3% in those with chronic atrophic gastrins .Specimens with chronic active gastritls, mostry manitesting local active lesions,had HP detectable rate of as high as 80.2%、69. 7% of the specimene with HP were found to have obvious lymph follicles reaction within lamina propria in their gastric mucosa.Conclusions There is a close relationship between HP infection and Pediatric chronic gastritis(especially chronic active gastritis). Histopathologically, HP-associated gastritis is characterized by chronic and local active inflamations, and tbe formation of lymph follicles within lamina propria.