The aim of joint development and sharing of institutional repository is to reveal and use the primary literature in military colleges and universities, and help scientific researchers, teachers and students to discover and use such re-sources .The ideas on joint development and sharing of institutional repository in military colleges and universities were thus analyzed, and the related problems were elaborated with suggestions put forward for their solution.

AIM: To establish a method of determining eucalyptol, camphor, menthol and borneol in Ganmaokang Spray. METHODS: GC was conducted on a EC-WAX column(30 m?0.53 mm?1.2 ?m) and naphthalene was adopted as internal standard. RESULTS: Eucalyptol, camphor, menthol and borneol respectively showed a good linear relationship in the following ranges of concentration:0.384 48-0.987 12 mg/mL,0.240 6～(0.561 4 mg/mL,)0.367 56-0.857 6 mg/mL and 0.135 6-0.316 4 mg/mL. The average recoveries were obtained as 98.4%, 98.2%, 98.5% and 98.2%(n=5). CONCLUSION: The method is simple, sensitive, accurate and suitable for the determination of four constituents in Ganmaokang Spray.

Objective To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis (PFGE REA) and random amplified polymorphic DNA (RAPD) analysis for molecular typing of Neisseria gonorrhoeae isolates. Methods Genomic DNA from 36 Neisseria gonorrhoeae isolates were digested with SpeI and analysed by contour clamped homogeneous electric fields electrophoresis. The 36 isolates were also characterised by RAPD analysis with arbitrary primer OPA 03. Results 12～19 DNA fragments from 5 to 600kb were obtained among the 36 isolates after PFGE REA. The 36 isolates were discriminated into 20 PFGE patterns. Amplification of Neisseria gonorrhoeae DNA with primer OPA 03 produced 15 different DNA fragments (280～1900bp), and 3～9 fragments per strain could be seen. The 36 isolates showed 18 different RAPD patterns. Strains sharing common auxotypes and antibiotic spectrum could be differentiated by PFGE REA and RAPD analysis. General agreement was found between these two techniques. Isolates from different geographic areas and even some isolates in the same area showed considerable amount of DNA polymorphism. In addition, some isolates shared common or very similar patterns were unique to a given geographic area. Conclusion It is concluded that both PFGE REA and RAPD analysis are useful, sensitive molecular techniques for differentiation of clinical isolates of Neisseria gonorrhoeae. They should be helpful in the investigation of strain origin, clonal relation among strains and spread of antibiotic resistance. Compared with PFGE REA, RAPD analysis is faster, relatively simple and more economical.

BACKGROUND:The effects of posterior decompression fixation in the repair of thoracolumbar fracture are affirmative. The fixation was divided into short-segment and long-segment pedicle screw fixation according to different treatment methods. The therapeutic effects of above two methods are controversial in the clinic at present. OBJECTIVE:To compare and observe the anterior vertebral compression ratio and the changes in Cobb angle after posterior short-segment and long-segment pedicle screw placement fixation in the repair of thoracolumbar fracture. METHODS:120 patients with thoracolumbar fracture were enrol ed in this study, and assigned to two groups according to fixation methods. Study group received posterior short-segment pedicle screw fixation. Control group received posterior long-segment pedicle screw fixation. Anterior vertebral compression rate and Cobb angle were observed before fixation, immediately and 12 months after fixation in both groups. RESULTS AND CONCLUSION:There was no significant difference in anterior vertebral compression rate and Cobb angle between the two groups before fixation (P>0.05). Anterior vertebral compression rate and Cobb angle were significantly smal er in the study group than in the control group immediately and 12 months after fixation (P<0.05). Besides, surgical time and blood loss volume were less in the study group than in the control group (P<0.05). These results indicated that posterior short-segment pedicle screw fixation for treatment of thoracolumbar fracture can effectively improve kyphosis, recover vertebral compression degree, and its surgical time is short and blood loss volume during surgery is little, so it is better than posterior long-segment pedicle screw fixation.

Objective: To investigate the effect of soy isoflavones extract on bone loss induced by estrogen deficiency. Methods: 87 postmenopausal women were randomly assigned in single blind manner, to three treatment groups with daily intake of placebo, 84 mg and 126 mg soy isoflavones respectively. Additionally a positive control group was 10 women was given daily 2.5 mg estrogen. Bone mineral density (BMD) of the lumbar spine, neck of femur and Ward`s triangle were measured at baseline and post treatment after 24 wk . Results: BMD in all sites in both isoflavones treated and estrogen treated groups did not change significantly. However, the BMD of the lumbar spine decreased in the placebo group (P

Background miRNAs are a group of non-coding small RNA molecules,and they play an important role in regulating the apoptosis of LECs.The biological effects and mechanisams of miRNAs on LECs in agerelated cataract still need to be further elucidated.Objective This study was to investigate the anti-oxidative effects of miR-204 on age-related cataract in vitro.Methods The specimens of anterior lens capsules from agerelated cataract patients and normal donors were collected in Shandong Eye Institution and 20 subjects for each.The expression level of miR-204 was detected and compared between cataractous eyes and normol eyes by real-time fluorescence quantitative PCR (RT-qPCR).HLE-B3 cells,a human LEC line,were cultured,and the oxydative stress models of LECs were established by adding 200 μmol/L H2 O2 in the medium.The models were divided into model control group,miR-204 agomir group,agomir negative control group,miR-204 antgomir group and antagomir negative control group according to the difference of tranfected agents,and normal cells served as the control group.Twenty-four hours after transfection,the expression levels of miR-204 mRNA in the cells of various groups were detected by RT-qPCR to varify the transfection rate.Apoptosis rate of the cells was assayed by flow cytometry.The relative expression levels of TP53INP1 mRNA and p53 mRNA as well as their proteins were detected by RT-qPCR and Western blot,respectively.Results The mean expression level of miR-204 was significantly lower in the anterior lens capsules of cataractous eyes than that in the normal eyes (t=14.21,P＜0.05).The mean apoptosis rate of cells was 1.31±0.12,4.90±0.28,2.60±0.15,4.39±0.20,5.74±0.13 and 4.34±0.63 in the normal control group,model control group,miR-204 agomir group,agomir negative control group,miR-204 antgomir group and antagomir negative control group,respectively.The apoptosis rate was significantly increased in the model control group compared with the normal control group (t =-20.69,P＜0.01) and the apoptosis rate was also increased in the miR-204 antagomir group compared with antagomir negative control group (t =3.79,P＜0.05);while the apoptosis rate in the miR-204 agomir group was significantly declined in comparison with agomir negative control group (t=-12.20,P＜0.01).The relative expression levels of TP53INP1 and p53 mRNA and proteins in the cells were significantly higher in the model control group than those in normal control group (mRNA:t =6.44,11.71,both at P＜0.0I;protein:t =10.72,19.40,both at P＜0.01),and so were between the miR-204 antagomir group and antagomir negative control group (mRNA:t =4.07,3.74,both at P＜ 0.05;protein:t =7.18,10.58,both at P＜0.05).However,the expression levels of TP53INP1 and p53 mRNA and protein were significantly reduced in the miR-204 agomir group in comparison with the agomir negative control group (mRNA:t =-19.28,-10.58,both at P＜0.05;protein:t=-6.50,-6.36,both at P＜0.05).Conclusions miR-204 induces oxidative damage of age-related cataract via targeting TP53INP1,which suggests that the activation of TP53INP1-p53 signaling may be involved in the development of age-related cataract.

Objective\ The present study was carried out to examine the relationship between the distance of axotomy and axonal regeneration systematically,and the effect of a pre\|degenerated peripheral nerve(PN) graft on axonal regeneration of retinal ganglion cells(RGCs) axotomized at different distances. Methods\ The optic nerve(ON) was transected at 0\^5,1,1\^5,2,3 or 7mm from the optic disc and a normal (the normal group) or pre\|degenerated(the pre\|degeneration group) PN graft was transplanted onto the ocular ON stump in adult hamsters. Results\ In both groups,the number of regenerating RGCs 28 days after grafting decreased significantly when the distance of axotomy increased from 0\^5 to 7mm with a sharp decline between 0\^5 and 3mm.When the corresponding distance points between the two groups were compared,enhanced regeneration was observed at 2 and 3mm in the pre\|degeneration group. Conclusion\ These results show that the distance of axotomy on the ON of adult hamsters is critical in determining the number of regenerating RGCs.The beneficial effect of a pre\|degenerated PN graft on axonal regeneration of axotomized RGCs is conditional on the distance of axotomy.\;

In this article we discuss the correlation between the functional defection of beta cell and its pathogenesis in traditional Chinese medicine;we summarize numbers of studies on Chinese medicine's effect on beta cell in different aspects through ways of experiment and clinical research,thus reflecting the unique effect of Chinese medicine in curing diabetes mellitus and improving the function of beta cell and at last proposing the direction of further study on this subject.

Objective To investigate the prevalence of sick building syndrome (SBS) and explore the related risk factors in the people working in office environment in Beijing. Methods A cross-sectional study design was made in the present paper in April 2009. One hundred and two employees in 16 small and moderate offices in three different buildings in Beijing were investigated with the questionnaire for their subjective symptoms, environmental perceptions, psychosocial information and health situation. The indoor environmental factors of the buildings were monitored based on the standardized procedures at the same time. Generalized linear model and multilevel model were used for the data analysis. Results The prevalence rate of symptoms for sick building syndrome was 67.4%. The prevalence rate of SBS symptoms was positively associated with the self-feelings of the subject to high temperature, insufficient ventilation indoors, work stress, satisfaction for job and health complains, as well as with using photocopier in office by ANOVA(P

After the concept of data curation and its application in foreign countries were described , the key points of data curation for data service in special libraries and related problems were analyzed according to the overall needs and changes of users in special libraries.