Objective To investigate the quantitative relationship between the changes of the parameters of Three-Electrode System and the Current following through WE (Farad current and Nonfarad current ). Methods We built a model based on the driving voltage-current system,which could describe the parameters of system and present the influence of current by changing parameters. Results The results of studying two simulational models accord with experiments of electrical chemistry. Conclusion This study provides an estimated value and influence of the electrochemistry noises,which is significant for data accuracy and anti-jamming of electrochemistry products.

Repeat cervical cytologic testing,human papillomavirus(HPV)testing and immediate colposcopy are currently the most general managements in women with atypical squamous cells of undetermined significance(ASCUS)result.However,the efficacy of these three methods is hampered by strong subjectivity,high false negative and positive rates.With the development of the aetiology of cervical cancer,there is good evidence that biomarkers for persistent HPV infection,HPV integration,instability of host genes and malignancy triage ASCUS more objectively and efficiently.The three-dimensional multiparameter biomarker-based detection may be the predominant method for the triage of women with abnormal cervical cancer screening result.

Objective To investigate the prevalence of SEN virus(SENV)infection in patients with chronic hepatitis gravis before and after plasma exchange,and its influences on liver function.Methods Two subtypes of SENV,SENV-D and SENV-H were detected in sera from 40 patients with chronic hepatitis gravis before and after plasma exchange.The changes of liver function were also tested before and after plasma exchange.Data analysis was done by t test and ehi-square test.Results Among 40 patients with chronic hepatitis gravis,SENV-D positive rates before and after plasma exchange were 27.5%(11/40)and 42.5%(17/40),respectively,and SENV-H positive rates before and after plasma exchange were 27.5%(11/40)and 50.0%(20/40),respectively,which were all significantly different(X2=4.17,X2=7.11,both P<0.05)between before plasma exchange and after plasma exchange.And after the treatment of plasma exchange,serum alanine aminotransferase(ALT)level was(164±75)U/L in SENV positive patients,which was significantly higher than that in SENV negative patients[(48±12)U/L,t=3.13,P<0.05].But there were no significant differences of other liver function indexes between SENV positive and negative groups.Condusions SENV can be transmitted via plasma exchange.SENV coinfection may influence the restoration of liver function in patients with chronic hepatitis gravia after the treatment of plasma exchange.

Objective To discuss the ultrasonic anatomy of gastrocnemius and its substructures in normal population. Methods Eighty gastrocnemius in 40 volunteers were scanned by real time high frequency ultrasound. Sonograms of medial and lateral heads of gastrocnemius were acquired. Plume angles between medial and lateral heads of gastrocnemius were measured at condition of rest, 5 kg and 10 kg isometric contraction. Both dominant and non-dominant legs were evaluated. Differences of plume angles were compared by ANOVA in different conditions and by t test in different legs. Results At the upper part of the muscle, both medial and lateral heads of gastrocnemius could be divided into muscular compartment, shallow compartment and deep compartment by hyperechoic intra-muscular septa with clear margin. The septa of lateral heads presented as hyperechoic side-lying′T′, while the septa of medial heads presented as hyperechoic side-lying-′T′. Vascular signals could be detected in these hyperechoic septa. The plume angle at the distal part of the lateral head of gastrocnemius was composed of shallow compartment attaching to the Achilles tendon, and that of the medial head was composed of medial muscular compartment attaching to the tendon. At rest, 5 kg and 10 kg isometric contraction, plume angles of lateral heads were (13.36±3.20)°, (13.32±3.30)° and (12.75±3.20)°, and plume angles of medial heads were (8.69±3.30)°, (8.59±2.99)° and (8.65±3.20)°. Under the same condition, plume angles of medial heads were larger than those of lateral heads and the difference was statistically signiifcant (t=9.09, 9.50 and 8.10, all P<0.01). Changes of plume angles between rest and different weight bearing conditions were small, and differences were not statistically signiifcant (F=0.89 and 0.02, P=0.41 and 0.98). Plume angles of medial heads in dominant legs and non-dominant legs were (13.66±3.60)° and (13.30±2.84)°, and those of lateral heads were (8.71±3.48)° and (8.80±3.35)°. The plume angles of medial heads were larger in dominant legs than those in non-dominant legs, while the plume angles of lateral heads were smaller in dominant legs than those in non-dominant legs. However, both differences were not statistically signiifcant (t=0.70 and 0.87, P=0.48 and 0.17). Conclusions The anatomical characteristics of medial and lateral heads and compartments of gastrocnemius can be clearly depicted by high frequency ultrasound. Plume angles can also be accurately measured.

Objective To observe the therapeutic effect of modified Buyang Huanwu decoction combined with betahistine hydrochloride in the treatment of vertebral-basilar artery ischemic vertigo and its influence on hemorheological index.Methods 80 patients with vertebral-basilar artery ischemic vertigo were randomly divided into observation group and control group,40 cases in each group.The control group was given betahistine hydrochloride 12 mg,three times a day.The observation group was given modified Buyang Huanwu decoction on the basis of the control group, 4 weeks for a course.The DARS and DHI were measured before and after 4 weeks of treatment.The blood flow velocity was detected by transcranial Doppler ultrasonography, the difference of clinical curative effect between the two groups was compared.Results After treatment, the DARS and DHI scores of the observation group and control group were significantly decreased(t=14.716 and 5.258,20.662 and 14.609,all P<0.01).The DARS and DHI scores in the observation group were significantly lower than those in the control group (t=3.644,7.116,all P<0.05).After treatment,the average blood flow velocity of the vertebral artery, right vertebral artery and basilar artery in the observation group and the control group were significantly increased(t=11.216 and 7.447,10.103 and 7.204,11.303 and 6.642,all P<0.01).The average blood flow velocity of the vertebral artery, right vertebral artery and basilar artery in the observation group were significantly higher than those of the control group (t=4.405,4.761,5.824,all P<0.01).The total effective rate of the observation group was 95.00% (38/40),which was significantly higher than that of the control group (77.50%,31/40), the difference was statistically significant (Z=-2.000,P<0.05).Conclusion Modified Buyang Huanwu decoction combined with betahistine hydrochloride is effective in the treatment of vertebro-basilar ischemic vertigo.It can improve the blood supply to the brain and relieve the symptoms of vertigo, which is worthy of popularization and application.

Abstract: It has long been thought that immune memory is an exclusive hallmark of adaptive immunity, but recent studies have shown that innate immunity also has characteristics similar to immune memory. Innate immune cells, upon being stimulated by pathogens such as lipopolysaccharide (LPS) and β-glucan, can develop a form of immune memory. Upon a secondary encounter with the stimulus, these innate immune cells can produce a stronger immune response, providing nonspecific protection. This immune phenomenon is termed "trained immunity". The mechanism of trained immunity mainly involves the epigenetic reprogramming and metabolic reprogramming of innate immune cells and the interaction between them. This article reviews the main research progress and mechanism of pathogen infection-induced trained immunity.

Objective To study the relationship between the virus content of high risk HPV and cervical intraepithelial neoplasia(CIN).Methods 450 patients with cervical intraepithelial neoplasia (CIN) were selected as the observation group ,450 healthy women at the same time were the control group ,then the positive rate and content of the control group and observation group with different high risk HPV types were detected and compared ,then the detection results of observation group with different CIN classification were compared too ,and the relationship of the detection indexes between the CIN were analyzed by the Logistic analysis .Results The HPV DNA positive rate of observation group with different high risk types were 9.11%,6.67%,6.67%,6.67%and 6.00%,respectively,and they were all higher than those of control group (χ2 =6.871,6.240,6.240,6.240,5.871,all P<0.05),and the positive rate of patients with different CIN classification had obvious differences too ,the virus content and constitution of observation group were respectively (301.35 ±32.67) Copies/mL and 11.56%,10.22%,9.56%,and they were all obviously higher than those of control group (t=6.276,χ2 =7.132,6.915,5.878,all P<0.05),and the content of patients with different classification had obvious differences ,all P<0.05,there were all significant differences ,and the item had close relationship to CIN by the Logistic analysis .Conclusion The high risk HPV virus content of patients with cervical intraepithelial neoplasia shows abnormal state ,and which have close relationship to the disease .

Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Mining ; Noninvasive Ventilation ; Pneumoconiosis ; rehabilitation ; Treatment Outcome

The clinical teaching of ICU is a new challenge.According to the problem encountered in the practice we should improve and better the teaching methods such as asking students to pay more attention to the combination of substructural subjects and critical medicine and having a clinical macrocosm thinking,good responsibility,and the "intensive" concept during their work so that student can learn more initiatively and get more as well.

OBJECTIVE:To investigate the effects of triptolide(TP)on the proliferation of fibroblast-like synoviocytes(FLS) from patients with rheumatoid arthritis(RA)in vitro. METHODS:5 RA patients received knee arthroplasty or synovectomy to ob-tain synovial tissue. FLS was isolated,cultured and identified,and then incubated in the presence of TP [0 (blank control),50, 100 and 200 nmol/ml] for 24,48 and 72 h. The effects of TP on FLS was evaluated by MTT,and then proliferation inhibitory rate was calculated;flow cytometry was used to detect the apoptosis and cell cycle of FLS. RESULTS:The inhibitory rates of TP(50, 100 and 200 nmol/ml)on the proliferation of FLS were 17.46%-52.56%,which was positively correlated with drug concentration. Compared with blank control group,100 and 200 nmol/ml TP could increase the percentage of cells in G0/G1 phase and decrease the percentage of cells in S phase,with statistical significance(P<0.05);200 nmol/ml TP could induce cell apoptosis. CONCLU-SIONS:TP could inhibit the proliferation and also could induce the apoptosis of FLS in RA patients in vitro,which may be one of its mechanism for treating RA.