Carcinoma, Hepatocellular ; therapy ; Drug Delivery Systems ; methods ; trends ; Genetic Therapy ; Humans ; Liver Neoplasms ; therapy ; Receptor, Epidermal Growth Factor ; administration & dosage

Carcinoma, Hepatocellular ; therapy ; Humans ; Liver Neoplasms ; therapy

Combined Modality Therapy ; Humans ; Liver Neoplasms ; therapy

Carcinoma, Hepatocellular ; therapy ; Chemoembolization, Therapeutic ; Humans ; Liver Neoplasms ; therapy

Antineoplastic Agents ; therapeutic use ; Carcinoma, Hepatocellular ; pathology ; surgery ; therapy ; Catheter Ablation ; methods ; Chemoembolization, Therapeutic ; Combined Modality Therapy ; Hepatectomy ; methods ; Hepatic Artery ; Humans ; Lung Neoplasms ; pathology ; surgery ; therapy ; Neoplasm Staging ; Radiotherapy, Conformal

Aim To study the effects of preconditioning with desflurane, sevoflurane and isoflurane on adenosine triphosphate (ATP) in anoxia-reoxygenation myocardial cells. Methods Rat ventricular myocytes, cultured for 4～5 days, were randomly allocated to five groups: Control group, anoxia-reoxygenation group and groups preconditioned with 1.5 MAC desflurane, sevoflurane or isoflurane following anoxia-reoxygenation. The content of intracellular ATP ,the activities of lactic dehydrogenase(LDH) and creatine kinase(CK), and the cell viability were measured at the end of experiment.Results Preconditioning with 1.5 MAC desfllurane, sevoflurane or isoflurane significantly attenuated the great reduction in ATP and cell viability and the increase of LDH and CK caused by anoxia-reoxygenation. There was a positive correlationship between ATP and cell viability,and a negatiue correlationship between LDH and CK (r was 0.83, -0.87 and -0.82 respectively, P

Objective To investigate the effect of miR-210 on angiogenesis of human renal clear cell carcinoma . Methods Detect the expression of miR-210 in 40 cases of renal clear cell carcinoma tissues , and analyze the rela-tionship between the expression of miR-210 and microvessel density ( MVD) .miR-210-ASO was lipotransfected in-to renal clear cell carcinoma cell line 786-O.RT-qPCR was used to verify the transfection effect .The effect of the supernatant of control group , negative control group and miR-210-ASO group tumor cells on the lumen formation of human umbilical vein endothelial cells ( HUVEC) was observed in a 3-D culturesystems .The transplantation tumor model of nude mice was established , and the effect of miR-210 on the formation of the transplanted tumor micro vessel was observed by endomucin and VEGF immunofluorescence staining under laser scanning confocal micro -scope.Results The expression of miR-210 was positively correlated with microvessel density in renal clear cell carcinoma ( P<0.05 ) .The expression of miR-210 was significantly decreased in 786-O cells transfected with miR-210-ASO( P<0.05 ) .The lumen formation of HUVEC cells co cultured with miR-210-ASO group cell supernatant was significantly less than that of control group and negative control group ( P<0.05 ) .The tumor volume of miR-210-ASO group was less than that of the control group , and the number of the micro vessel and the VEGF expres-sion were significantly less than that of the control group ( P<0.05 ) .Conclusions Inhibition of miR-210 can suppress blood vessel formation in renal clear cell carcinoma .

Objective To explore the value of laparoscopic partial nephrectomy for children with duplex kidney.Methods Between June 2006 and February 2009,we performed laparoscopic partial nephrectomy on totally 21 children with duplex kidney.Three or four trocars were used for the operation.Through transabdominal approach the peri-renal tissues were freed and the vessels supplying the duplicated kidney were ligated.Afterwards,we resected the duplicated kidney,as well as the ureter,and a subperitoneal drainage tube was indwelt.Results The operation was completed in all of the 21 cases without conversion to open surgery.The mean operation time for laparoscopy was 190 min(range,130 to 210 min).The intraoperative blood loss ranged from 5 to 10 ml.No patient received blood transfusion.Liquid diet was given in 8 to 10 hours after the operation.In this series,the postoperative hospital stay ranged from 5 to 7 days with a mean of 6.2 days.Follow-up was available for 3 to 28 months in the cases(mean,20.2 months).During the period,the clinical symptoms disappeared and B ultrasonography showed no abnormalities.Conclusions Providing larger operation space and better exposed surgical field,laparoscopic partial nephrectomy for children with duplex kidney is feasible and safe with quicker recovery.

砄bjective: To evaluate the effect on a transpalatal arch on reinforcing anchorage. Methods: 12 cases with ClassⅡ Division 1 malocclusion were selected and treated with a transpalatal arch for reinforcing anchorage. Results: ①The forward displacement of the first maxillary molar (6 Ptmo, 6 So, 6CR So and 6AP So) , the backward displacement(1 Ptmo and 1 So ) and the reduction of the axial inclination (1 NA and 1 SN) of the upper incisors were increased( P 0.05);③The forward displacement of the first maxillary molar was less than one third of the extraction space ( P

Objective To explore the curative effect of laparoscopic repair of congenital diaphragmatic hernia in children. Methods Three newborns(age,3~24 hours) and 8 infants(age,2~24 months) with congenital diaphragmatic hernia underwent 3-port or 4-port laparoscopic repair from June 2002 to December 2005 in this hospital.The hernial content was reduced and the defect was repaired by using intermittent sutures.Results The laparoscopic operation was successfully completed in 10 cases,while a recurrence was encountered in 1 newborn 3 days after the laparoscopic operation and then was cured by a re-operation of laparoscopic repair.The operative time ranged 55~180 min(mean,100 min),and the intraoperative blood loss was 1~2 ml.All the patients began to take food at 1~2 days after operation.Follow-up examinations in the 11 patients for 9~24 months(mean,16 months) showed a normal position of the diaphragm under fluoroscopy. Conclusions Laparoscopic repair of pediatric congenital diaphragmatic hernia is a feasible and safe technique,with advantages of clear visualization and thorough abdominal exploration.