Objective To understand the health status of the staff at a vocational technology college and analysis the cause of health problems.Methods The data were analyzed and had been statistical processing according to the physical examination reports of a total of 707 Changzhou hygiene teaching staff from 2011 to 2013.Results The top ten diseases and total prevalence were as follows:Abnormal electrocardiogram 48.44％ (295/609),dyslipidemia 39.97％ (247/618),the male prostate hyperplasia 38.82％ (73/188),the female mammae lobule hyperplasia and breast lump 29.05％ (147/506),hypertension 27.44％ (152/554),the female uterine myoma 21.94％ (111/506),fatty liver 21.77％ (133/611),hepaticcysts 20.62％ (126/611),gallbladder polyps and cholecystitis18.66％ (114/611),elevated blood sugar 9.53％ (59/619).Dyslipidemia and abnormal of electrocardiogram were on the rise overall during the three years.Dyslipidemia was releated to sex and age,the number that plasma lipid of women aged from 55 to 64 reach appropriate level was lowest.Conclusions The faculty of vocational colleges have higher incidence of chronic diseases.Women group aged from 55 to 64 have highest incidence of dyslipidemia;more active,targeted measures need be taken.

This paper outlines some molecular marking technology based on rDNA sequences and several DNA fingerprinting technology (RAPD, ARDRA, AFLP, REP/ERIC-PCR) used in classification, identifica-tion and diversity research in lactic acid bacteria. The principles, methods and progress in recent years of these technologies were also introduced. At the same time, this paper also compares the advantages and dis-advantages of these methods. People should choose suitable method according to their purposes.

Objective]To explore Professor YE Jin’s clinical experiences for dwarfism treatment based on spleen-insufficiency constitution related theory. [Methods]From the concept of dwarfism based on TCM, etiology and pathogenesis, key symptoms of spleen-insufficiency constitution and features medication to analyse the academic perspective and clinical experience of Professor YE Jin in the treatment of dwarfism, and list 1 proven case.[Results]Professor YE Jin thinks there’re 5 types constitutions of dwarfism, and attaches importance to adjust the unbalanced constitution of the patients. She thinks spleen-insufficiency constitution is basis of the other four types. She does well in enlivening and invigorating the spleen and activating spleen-energy.[Conclusion] Professor YE Jin has unique clinical experience for dwarfism treatment based on spleen-insufficiency constitution related theory, with significant effect.

OBJECTIVE To evaluate the efficacy of combined therapy on chronic rhinosinusitis(CRS). METHODS Thirty cases with CRS in stage Ⅱ-2 were treated by FESS. At 1,3,6-month after operation,endoscopy and nasal mucosa biopsy were performed and the clinic symptoms were reassessed. RESULTS Although fine mucosal epithelization was found in all cases at 6 month after operation,mild edema of mucosa and muciform secretion still existed in 10 cases. Mucociliary transport rate (MTR) was 3.63?1.74 mm/min before operation,and was 6.27?1.31 mm/min at 6-month after operation. Compared with that before operation,the MTR was significantly improved whereas it had not rehabilitated to normal status. The decrease of inflammatory cells and alleviation of submucosal edema were observed in the mucosa of maxillary sinus. Nevertheless,the number of goblet cells and submucosa glands did not improve significantly. The ultrastructure of the mucosa was not recovered completely at 6 month after operation. CONCLUSION With combined therapy on CRS for 6 months, clinical cure can be achieved,but mucosal inflammatory reaction is still existed.

[Objective]The paper was designed to introduce the Professor YE Jin's personal clinical experience which can provide an effective means for other pediatrician.[Method]By introducing the Professor YE Jin's personal clinical experience,we summarize the effective therapies about the infantile recurrent abdominal pain(RAP). [Result]According to the Professor YE Jin, the pathomechanism of RAP includes mainly cold stagnation, the indigestion, parasite and Qi stagnancy. RAP caused bycoldcan be treated from warming the middle-energizer and fending off the cold,RAP resulting from retention of food can be cured by removing the retention and invigorating the stomach, RAP incurred by ascaris can be cured by relieving ascariasis,expelling parasite and purgation simultaneously,and RAP resulting from Qi stagnation can be cured by soothing liver, regulating qi activity, rather than relieving pain merely. Based on the above-mentioned theory,she tends to use Liquorice and prefers Paeonia a lot in curing the disease, that is always effective in clinic as a result.The recurrence rate is also lower.One case was provided for verification.[Conclusion]Professor YE Jin thinks that warming the middle-energizer and fending off the cold, removing the retention and invigorating the stomach, the RAP can be improved the curative effect,and that can provide another idea for other clinicians.

[Objective]This paper mainly introduced Professor YE Jin 's experience in treating girls with precocious puberty from liver syndrome differentiation and characteristics of prescription medication.[Methods] On account of three main symptoms of girls with precocious puberty at first visit, including thelarche, leukorrhea and menses, we explore the relation between liver and the causes of this disease from the viscera-state doctrine as well as the meridian theory. Then introduce Professor YE Jin's methods and empirical medication in treating girls with precocious puberty based on liver. In order to analyse her experience, a clinical case was reported. [Results]Professor YE holds that the pathogenesis of girl 's precociouss puberty closely related to liver. The deficiency of liver-yin and kidney-yin is the basic pathogenesis of precocious puberty, which is often accompanied with liver qi depression or even liver qi depression transforming into fire. On the basis of nourishing yin and purging fire, Professor YE emphasizes treating precocious puberty from liver. In practical terms, flexibly applying the ideas of softening, soothing and clearing liver can effectively improve the clinical symptoms. She makes the selection and compatibility of Chinese medicine comforming to the characteristics of children 's physiology and pathology and values the protection of vital energy. The reported case specifically demonstrates treating a girl 's thelarche from liver syndrome differentiation as well as the empiric therapy. [Conclusion]Professor YE Jin's experience in treating girls with precocious puberty is well worth learning for its significant curative effect.

Objective To analyze the current situation and the trend of the death due to foreign body in airway in children less than 5 years old in China from 1990 to 2017. Methods Using the mortality data of foreign body in airway in China from 1990 to 2017 from the Global Disease Burden 2017 (data covered 31 provinces, autonomous regions, municipalities, as well as Hong Kong and Macau Special Administrative Regions, excluding Taiwan Province) to describe the death status of children under 5 years old due to foreign body in airway in China, and to calculate the relative change and 95% uncertainty interval ( UI ) of mortality rate and proportion of children under 5 years old due to foreign body in airway between 1990 and 2017. The robust linear regression was performed with the first?order lag of mortality, and the supremum Wald test was used to explore whether the mortality trend had obvious structural changes around a certain time point. Results In 2017, the mortality rate of children under 5 years old due to foreign body in airway in China was 8.57/100 000 (95%UI : 7.41/100 000-9.57/100 000), and there was no significant difference between boys (9.05/100 000, 95%UI : 6.82/100 000-10.23/100 000) and girls (8.02/100 000, 95%UI : 7.02/100 000-9.12/100 000). The supremum Wald test showed that there was a structural change around the year of 2005 (P<0.001) in terms of the mortality rate of foreign body in the airway among children under 5 years old, which depicted a relatively stable trend before 2005 and a significant downward trend after 2005. Compared with 1990, the mortality rate of foreign body in airway among children, boys and girls under 5 years old in 2017 decreased by 49.32% (95%UI : 37.78% - 65.41%), 41.22% (95%UI :25.68%-68.26%) and 56.91% (95%UI: 44.78%-66.70%), respectively; the mortality proportion of foreign body in airway among increased by 153.33% (95%UI : 75.99% - 204.56%), 171.05% (95%UI :48.75%-239.63%) and 137.90% (95%UI: 86.62%-198.09%), respectively; the rank of foreign body in airway in all disease increased by 6 and became the first leading cause of injury from the second. Conclusion From 1990 to 2017, the mortality rate of foreign body in airway among children, boys and girls under 5 years old in China generally showed a downward trend. Compared with 1990, the mortality proportion of foreign body in airway among these population increased by a relatively large extent in 2017.

Objective To analyze the current situation and the trend of the death due to foreign body in airway in children less than 5 years old in China from 1990 to 2017. Methods Using the mortality data of foreign body in airway in China from 1990 to 2017 from the Global Disease Burden 2017 (data covered 31 provinces, autonomous regions, municipalities, as well as Hong Kong and Macau Special Administrative Regions, excluding Taiwan Province) to describe the death status of children under 5 years old due to foreign body in airway in China, and to calculate the relative change and 95% uncertainty interval ( UI ) of mortality rate and proportion of children under 5 years old due to foreign body in airway between 1990 and 2017. The robust linear regression was performed with the first?order lag of mortality, and the supremum Wald test was used to explore whether the mortality trend had obvious structural changes around a certain time point. Results In 2017, the mortality rate of children under 5 years old due to foreign body in airway in China was 8.57/100 000 (95%UI : 7.41/100 000-9.57/100 000), and there was no significant difference between boys (9.05/100 000, 95%UI : 6.82/100 000-10.23/100 000) and girls (8.02/100 000, 95%UI : 7.02/100 000-9.12/100 000). The supremum Wald test showed that there was a structural change around the year of 2005 (P<0.001) in terms of the mortality rate of foreign body in the airway among children under 5 years old, which depicted a relatively stable trend before 2005 and a significant downward trend after 2005. Compared with 1990, the mortality rate of foreign body in airway among children, boys and girls under 5 years old in 2017 decreased by 49.32% (95%UI : 37.78% - 65.41%), 41.22% (95%UI :25.68%-68.26%) and 56.91% (95%UI: 44.78%-66.70%), respectively; the mortality proportion of foreign body in airway among increased by 153.33% (95%UI : 75.99% - 204.56%), 171.05% (95%UI :48.75%-239.63%) and 137.90% (95%UI: 86.62%-198.09%), respectively; the rank of foreign body in airway in all disease increased by 6 and became the first leading cause of injury from the second. Conclusion From 1990 to 2017, the mortality rate of foreign body in airway among children, boys and girls under 5 years old in China generally showed a downward trend. Compared with 1990, the mortality proportion of foreign body in airway among these population increased by a relatively large extent in 2017.

OBJECTIVE: To investigate the application of near infrared spectroscopy in rapid and quantitative determination of multiple quality indicators in concentration process of Bufo bufo gargarizans Cantor extract. METHODS: NIR transmission spectra of concentrated solutions were collected in rectangular quartz cuvette with different optical pathlengths (2 and 5 mm). Calibration models of density, water content and indole alkaloids were developed by partial least squares regression (PLSR). The established models were applied to predict the unknown samples to test the performance of the models. RESULTS: The correlation coefficients of the density, water content and indole alka. The residual predictive deviations (RPD) were all above 3, and the relative standard errors of predictions(RSEP) were less than 10%, indicating the satisfactory model performance and predictive ability. Moreover, better calibration results were obtained with an optical path length of 2 mm for density and water content, and 5 mm for indole alkaloids. The results revealed that the optical path length has influence on the NIR analysis results, and the appropriate path length for the NIR analysis should be determined by comparison analysis. CONCLUSION: NIR spectroscopy technique provides a novel efficient and environmental friendly approach for the fast simultaneous determination of three key quality indicators (density, water content and indole alkaloids) in the concentration process of Bufo bufo gargarizans Cantor extract. Copyright 2012 by the Chinese Pharmaceutical Association.