Background and purpose:Nasopharyngeal carcinoma is sensitive with radiotherapy, and it is of great signiifcance to study the resistance mechanism. This study aimed to analyze the expression of TNFAIP3 in the radiotherapy of nasopharyngeal carcinoma, the difference between radiosensitivity and radioresistance, the relationship with the occurrence and development of radioresistance.Methods:Detection of TNFAIP3 mRNA expression was carried out by TNFAIP3 multi-point labeled DIG probein situ hybridization.Results:In radiosensitivity and radioresistance of nasopharyngeal carcinoma, the moderately and strongly positive TNFAIP3 mRNA expression rates were 15% and 45%, (χ2=5.274,P=0.021 6), there were statistical significance. In the radioresistat nasopharyngeal carcinoma, TNFAIP3 mRNA moderately and strongly positive expression was positively correlated with TNM stage. In the degree ofⅢ-Ⅳ tumor, it had moderately and strongly positive expression rate of 48.28%.In the degree ofⅠ-Ⅱtumor, it had moderately and strongly positive expression rate of 36.36% (χ2=33.240,P<0.005); The expression rates in distant metastasis and no distant metastasis were 81.81% and 31.03% (χ2=6.385,P=0.011 5), the difference had statistical signiifcance.Conclusion:TNFAIP3 has antitumor effect, and it is correlated with the occurrence and development of radioresistant in nasopharyngeal carcinoma.

To keep vocational schools distinctive and vigorous,top priority should be placed in teachers'training and teaching,making practice skill training systematic,embodying practical open professional principle based on vocational education in the process of practice teaching,by the teachers'guidance and stimulating students to actively participate in practice,reforming teaching ways and means.

Objective:Through the analysis of the basic situation of the medical service of the psychiatric inpatients in Beijing from 2003 to 2015,it provided the basis for rational allocation of mental health resources and reasonable utilization of medical expenses.Methods:Descriptive statistical method was used to analyze the annual discharged patients,average hospitalization days and hospitalization expenses of inpatients with mental illness.Results:The annual discharged patients were increasing gradually.The number of discharged patients increased from 7861 to 24898 during 2003-2015.The annual average growth rate was 10.08％.The average length of stay in hospital since 20ll was lower than before,which were about 40 days in recent years.The average hospitalization expenses per day were also increasing.It increased from 131.04 yuan to 461.02 yuan during 2003-2015.The annual average growth rate was 11.05％.Conclusion:The demand of mental illness patients for hospitalization was gradually increasing.The hospitalization demand of patients with mental illness was increasing.The medical expenses quickly increased.It needed to adjust the payment policy to promote the rational and effective utilization of medical expenses.

AIM:To investigate the role of SDF-1? in migrating of bone marrow stromal cells to the injured areas. METHODS:Ischemic brain lesion model was created in rats by permanent middle cerebral artery occlusion (MCAO). 48 SD rats were divided randomly into 2 groups. Group 1:phosphate buffered saline (PBS 1 mL) for control (n=25); Group 2:BMSCs (2?106) were injected intravenously at 24 h after MCAO (n=24). After propagated in BMSCs,Ad5/F35 GFP (green fluorescent protein) was infected to BMSCs. The expression of SDF-1? (stromal cell-derived factor-1?) mRNA in the penrumbral tissue was assayed by real-time quantitative PCR. The expression of CXCR4 on MSCs was detected by flow cytometry. Confocal microscopy was used to detect the GFP-labeled MSCs migration. RESULTS:Ad5/F35 GFP signals was observed in almost infected BMSCs. The expressions of SDF-1? mRNA in the thalamus and hippocampus of the ischemic brains were peaked at 3rd day after stroke,followed by a decrease at 14th day post-ischemia. The expression of SDF-1? mRNA in the cortex of the ischemic brains was peaked at 7th day post-ischemia,still at high level at 14th day post-ischemia. The median percentage of surface CXCR4 expression in BMSCs was 14%. GFP labeled BMSCs were detected in the origination of the middle cerebral artery (olfactory area) at 6 h,after 3 days in the prenumbra tissue such as thalamus,and in the cortex more labeled cells were found after 14 d post-ischemia.CONCLUSION:BMSCs can pass through the blood brain barrier of ischemic rats. Its mechanism might be associated with the expression of SDF-1? in the ischemic brain.

Objective To guide the therapy for cyanide poisoning patients on plateau, the effect of hypoxia on the efficiency of 4-(N, N-dimethyl)-aminophenol (4-DMAP) in ferrihemoglobin (MHb) formation was investigated. Methods Rabbits were raised in hypoxic condition (4500m altitude) as acute-hypoxic animal model, and MHb concentration was determined by spectrophotography at the length of 635nm after 4-DMAP injection. Results There was no difference in hemoglobin content and MHb concentration between acute acutehypoxia group and controls. But in hypoxic rabbits, MHb formation as induced by 4-DMAP was improved distinctly. 30%-40% MHb concentration formation (optimal anti-cyanide concentration) was induced by 20mg/kg 4-DMAP in hypoxic rabbits, compared to 25-30mg/kg in control rabbits. However, when hypoxic rabbits received 25-30mg/kg 4-DMAP, which was the optimal dose for treatment of cyanide poisoning in normoxic animals, the animals manifested even worse symptoms of hypoxia, eren death occurred. Moreover, the level of MHb in acute-hypoxic models maintained longer than that in controls. The effect of 4-DMAP in MHb formation was closely related with duration of hypoxia. During hypoxia period from 1d to 5d, MHb concentration was increased gradually along with the duration of hypoxia, peaking on the 5d, and it started to decline on 7d, but it was still higher than that in control group. Conclusion Hypoxia enhances the effect of 4-DMAP in MHb formation and lowers the metabolism of MHb.

Objective To evaluate the effect of Orem intervention on the living quality and psychological state of elderly pa-tients with pulmonary tuberculosis.Methods Sixty-eight elderly patients with pulmonary tuberculosis were randomly divided into control group (n=31) and observation group (n=37).Orem intervention was applied in the observation group, while routine nurs-ing was used in the control group.The score of short-form-36 health survey (SF-36), self-rating depression scale (SDS), self-rating anxiety scale (SAS) and satisfaction were compared between the two groups.Results After the intervention, the SF-36 scores of observation group were significantly increased in 8 dimensions, compared with control group , with statistically significant differences (p<0.05).Compared with the control group, the SDS and SAS scores of the observation group were significantly de-creased statistically (p<0.05).The satisfaction rates of the control group and the observation group were respectively 77.4% (24/31) and 94.6% (35/37), with statistically significant differences (p<0.05).Conclusion Orem intervention can effectively im-prove the living quality and psychological state of elderly patients with pulmonary tuberculosis , worth clinical promotion.

Objective To explore the influence of QCC on the infection of orthopedic aseptic operation and healthcare workers in the operating room. Methods A total of 170 patients undergoing orthopedic aseptic operation from January 2015 to June 2015 were chosen as QCC group and 164 patients from June 2014 to December 2014 as control group. The wound infection rate,increased rate of WBC and temper-ature,the responsibility,initiative,problem solving skills,self-confidence,harmony,cohesion of doctors and nurses between two groups were recorded. Results The wound infection rate(2. 4%), increased rate of WBC(2. 4%) and temperature(1. 2%) in QCC group were signifi-cantly lower than those of control group(5. 5%,9. 1%,6. 7%). Moreover,the responsibility(4. 6 ± 0. 6) point,initiative(4. 4 ± 0. 3) point, problem solving skills(4. 1 ± 0. 8) point,self-confidence(4. 3 ± 0. 7) point,harmony(4. 8 ± 0. 9) point and cohesion (4. 2 ± 0. 4) point of doctors and nurses in QCC group were significantly higher than those of control group[(2.9±0.7) point,(2.3±0.5) point,(2.5±0.5) point,(2. 8 ± 0. 6) point,(3. 1 ± 0. 4) point,(1. 8 ± 0. 3) point,respectively)]. Conclusion QCC could effectively reduce the infection rate of orthopedic aseptic operation,and improve the overall quality of the participants health care workers in the operating room.

Objective To design an electrostimulator for cerebellar fastigial nucleus stimulus, which is controlled with singlechip system and using EEG(Electroencephalogram)to modulate amplitude of the carrier wave. Methods The EEG with instrumentation amplifier of high CMRR (Common Mode Rejection Ratio) and some filters are collected. The electric insulation is achieved by using linear optocouplers on the parts of input and output. Results The stimulator can solve the problem of adaptability for wave to simulate the body, and is safe, non -invasive, and of obvious therapy for ischemic cerebrovascular disease. Conclusion The cerebellar fastigial nucleus stimulus can cure ischemic cerebrovascular disease obviously, and the modulation with EEG can solve the problem of adaptability for wave to simulate the body.

Objective: To investigate association between psychosocial factors and anxious and depressive disorders in patients with diabetes mellitus. Methods: 172 patients with type 2 diabetes were inquired with a self-administered questionnaire about their socio-demographic characteristics, perceived stress caused by the disease, coping styles, social support. At the same time, anxious and depressive disorders were assessed with HAD.Results:Among our sample, 35 (20.3%) patients with anxiety, 33 (19.2%) with depression. After adjusting for gender, age, marital status, educational level and income, multivariate logistic regression analysis found the following results. Perceived stress from "Worrying about to be harmed by the disease"(OR=1.76,95%CI 1.12~2.77),"Social/family crisis caused by the disease"(OR=1.85, 95%CI 1.18~2.92),"Worrying about declining in body/physical function"(OR=2.16, 95%CI 1.28~3.67), "Declined economic condition"(OR=2.03,95%CI 1.27~3.26)and "Negative coping styles"(OR=1.94,95%CI 1.26~2.97)were significantly associated with anxious disorders. The perceived stress from "Social/family crisis caused by disease"(OR=1.79, 95%CI 1.09~2.96), "Worrying about declining in body/physical function"(OR=2.89, 95%CI 1.59~5.24)and "Negative coping styles"(OR=1.95,95%CI 1.23~3.11)were significantly associated with depressive disorders, while patients "Taking avoidant measures"(OR=0.52, 95%CI 0.30~0.90)were less depressive disorders.Conclusion: Anxious and depressive disorders in patients with 2 type diabetes were significantly associated with patients' perceived stress from the disease and their coping styles.

Objective To test the in vitro effect of the extract of Herba taraxaci on Demodex folliculorum. Methods Active Demodex folliculorum were obtained from patients with moderate to severe demodex infestation. Herba taraxaci and Radix stemonae were extracted respectively with 80% ethanol under 85℃, and a preparation with a concentration of 200mg/ml was made. The extractions were used in vitro to examine the anti-mite activity by observing time of killing mites. Physiological saline and Radix stemonae extraction served as blank control and positive control respectively. PH value of Herba Taraxaci extract was noted. Skin irritation test of normal and wounded skin and acute toxicity test were carried out with rabbit shin, and Herba taraxaci and 75% ethanol were served as experiment and control medicine. Results Motion and morphology of the mites considerably changed with the effect of Herba taraxaci extract. The time of mite-killing was (1.50?0.65)min with Herba taraxaci and (3.53?1.04)min with Radix stemonae respectively (P