Hereditary nonpolyposis colorectal cancer (HNPCC) is a distinct autosomal dominant syndrome, accounting for 5%-10% of the total colorectal cancer population. To date, we have noticed that patients with HNPCC inherit a germline mutation in one of the genes responsible for repair of DNA mismatch errors. Tumors of HNPCC show a high frequency of microsatellite instability. Identifying individuals afflicted with HNPCC has implications for surgical management, prognosis, follow up, and surveillance of HNPCC patients and family members at risk.

Purpose:To explore the clinicopathologic features of sporadic colorectal cancer patients with microsatellite instability(MSI) and the correlation between the microsatellite instability and the DNA ploidy.Methods:Detecting microsatellite instability with two sites(BAT25 and BAT26),conduct flow cytometry to analyze the DNA ploidy among 71 sporadic colorectal cancer patients and probe into the relationship between the microsatellite instability phenotype and the clinicopathologic characteristics as well as the DNA ploidy.Results:The positive rate of the microsatellite instability among sporadic colorectal cancer patients was 9.86 percent.The microsatellite instability phenotype was correlated with the tumor site and the histopathologic type and the differentiation grade with the P value less than 0.05,while it was independent of the gender,age,lymph node metastasis and Dukes' stage.The proportion of right colon cancers and poorly-differentiated adenocarcinomas of sporadic colorectal cancers showing microsatellite instability was higher than that of microsatellite stable ones.18 and 50 cases showed diploidy and aneuploidy respectively.5 cases of MSI were diploid,There was statistically significant correlation between the microsatellite instability phenotype and the DNA ploidy(P=0.012).Conclusions:Sporadic colorectal cancers with microsatellite instability were prone to occur in the right colon and to be poorly-differentiated adenocarcinomas and inclined to be diploid.

Objective To investigate the clinicopathologic features of sporadic colorectal cancers with CpG island methylator phenotype. Methods The methylation of the promotors of the five genes including p14, hMLH1, p16, MGMT and MINT1 were detected using methylation specific PCR in 71 patients to determine the clinicopathologic characteristics of sporadic colorectal cancers with CpG island methylator phenotype. Results Fifteen out of 71 (21. 1%) patients were positive for the CpG island methylator phenotype. The proportion of the right-sided colonic cancers(40. 0% vs. 12. 5% , P

Stroke is one of the most common diseases among middle and senior - aged people,and depressive disorder,which hinders severely recovery and prognosis,is one of the complications of stroke.This paper analyzes the efficiency of social intervention mode on patients with post - stroke depressive disorder,and comes up with the conclusion that social intervention mode can not only help to ameliorate the occurrence of post - stroke depressive disorder,but also promote the recovery of patients' nervous function.Therefore,the social intervention mode can be regarded as an effective means for medical treatment.

Objective To assess the effect of thoracoscopic surgery under laryngeal mask anesthesia and explore the nursing strategies. Methods Thirty-five patients from April to December in 2014 undergoing video-assisted thoracoscopic surgery (VATS) were given intubation anesthesia and another thirty-five ones undergoing the same surgery in 2015 received laryngeal mask anesthesia. The two groups were compared in terms of surgery conditions, surgery complication and ambulation time. Results No significant differences were found in surgery time or blood loss between two groups (P>0.05). The postoperative waking time in the mask anesthesia group was significantly shorter than that of the intubation anesthesia group (P<0.05), and ambulation time was significantly shortened (P<0.05). The rates of throat discomfort, hoarseness and gastrointestinal reactions were significantly lower. Conclusions Laryngeal mask anesthesia used in small thoracoscopic surgery for airway management is safe and feasible. Combined with training in respiration and limb function, better analgesia and nursing, it can keep away complications related to intubation anesthesia, shorten hospital stay and accelerate postoperative rehabilitation.

Objective To observe the effect of salt-partitioned moxibustion at Zhongwan (CV12) in treating belching due to chemotherapy.Method Subjects with chemotherapy-induced belching were randomized into an observation group and a control group, 30 in each group. The control group was intervened by intramuscular injection with Ritalin 20 mg, while the observation group was by salt-partitioned moxibustion at Zhongwan for 30 min in addition to the intramuscular injection.Result The total effective rate was 93.3% in the observation group versus 76.7% in the control group, and the difference was statistically significant (P＜0.05); the action latency in the observation group was (1.7±1.8)min in the observation group versus (15.6±4.2)min, and the difference was statistically significant (P＜0.01).Conclusion Salt-partitioned moxibustion at Zhongwan is significantly effective in treating chemotherapy-induced belching, and it’s easy-to-operate, efficient, and safe.

Child ; Child Day Care Centers ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Intestinal Diseases, Parasitic ; epidemiology ; prevention & control ; Male ; Nematode Infections ; epidemiology ; prevention & control ; Prevalence

OBJECTIVETo compare the efficacy between moxa salt packets at acupoints combined with tropisetron hydrochloride and single use of tropisetron hydrochloride for cisplatin chemotherapy-induced gastrointestinal reaction.

METHODSSixty patients with malignant tumor who met inclusive criteria and received chemotherapy for the first time were recruited and randomly divided into an observation group and a control group, 30 cases in each one. Between the first days and fifth day into the chemotherapy, the patients in the control group were treated with daily intravenous injection of tropisetron hydrochloride (5 mg), while patients in the observation group, based on the treatment of control group, were treated with moxa salt packets at Zhongwan (CV 12). The nausea and vomiting between the first days and fifth day into the chemotherapy were compared in the two groups, and the occurrence rates of adverse reactions within the first week into chemotherapy were recorded.

RESULTSBetween the second day and fifth day into the chemotherapy, the effective rate for nausea in the observation group was higher than that in the control group (all P < 0.05); between the third day and fifth day into the chemotherapy, the effective rate for vomiting in the observation group was higher than that in the control group (all P < 0.05); one week into the chemotherapy, the occurrence rate of constipation in the observation group was lower than that in the control group (P < 0.05).

CONCLUSIONThe moxa salt packets combined with tropisetron hydrochloride can effectively reduce the cisplatin chemotherapy-induced nausea and vomiting as well as the occurrence rate of delayed-type vomiting, and improve the constipation, which is superior to single use of tropisetron hydrochloride.

Acupuncture Points ; Adult ; Aged ; Antineoplastic Agents ; adverse effects ; Cisplatin ; adverse effects ; Female ; Gastrointestinal Diseases ; etiology ; therapy ; Humans ; Male ; Middle Aged ; Moxibustion ; Nausea ; etiology ; therapy ; Neoplasms ; drug therapy ; Vomiting ; etiology ; therapy

Purpose:To investigate the susceptible factors and treatment against cross infection in hospital of cancer patients.Methods:174 cases of cross infection in hospital of cancer patients from 1997 to 1999 were analyzed for infective site, infective agent and infectious bacteria. Results:174 cases of cross infection in hospital of cancer patients were found, which was 10.45% of the in paitents of the same period. The majority (108/174) had infection of tissues and organs within irradiation fields after radiotherapy. Respiratory tract infection rate was 76.43%. 16 cases (40.0%) were found to have secondary infection of digestive canal after chemotherapy in the 40 cases of infection of digestive canal. Bone marrow deression or chronic failure and long term decubitus were also causes of infection. In addition, mycotic infection was 43.42% (33/76) in bacteriological tests with bad prognosis.Conclusions:Cross infection in the hospital occurs that patients enfeebled by radiotherapy, chemotherapy, chronic bone marrow failure and long term decubitus. Attention should be paid to the possibility of mycotic cross infection in hospitals, as the prognosis could be quite serious. As soon as the diagnosis is confirmed, early and full dose treatment by anti mycotic drugs should be given.

Objective To evaluate the clinical outcomes of intravesical instillation of resiniferatoxin (RTX) for the treatment of overactive bladder(OAB). Methods 30 patients (28 females and 2 males) included in the study.The mean age was 39 years (range,21 to 60 years).The mean course of the illness was 6.9 years (range,2 to 30 years).The voiding diaries of pretreatment showed that frequency at day time varied from 9 to 26 times with a mean of 15.0 times;frequency at night ranged from 3 to 13 times with a mean of 6.4 times.Of the 12 patients undergoing cystoscopy 10 had chronic inflammation in both mucous and submucous.Based on biopsy 1 case was pathologically diagnosed as glandulous cystitis,2 as interstitial cystitis,and others as chronic inflammation.100 ml of RTX at a concentration of 100 nmol/L was intravesically instillated in all the patients and was retained within the bladder for 30 minutes. Results RTX instillation had no vesical irritation and other adverse effects,and no local anesthesia was required.The symptoms were improved immediately in all the patients after 1 day of RTX intervention.The decreases in both diurnal (5 to 15 times,mean 8.9 times) and nocturnal (0 to 5 times,mean 3.0 times) frequencies were significant(P