24 cases(25 eyes)of traumatic cataract with various complications were performed combined operations such as vitrectomy and extracapsular lensectomy from behind to leave the anterior capsule followed by imp- lantation of IOL,vitrectomy and removal of intraocular foreign body followed by secon- dary extracapsular extraction of the cataract and IOL implantation when the lens became opaque,and removal of intraocular foreign body with extracapsular extraction of the lens and IOL implantation.The position of incision,the method of anterior capsulotomy and nucleus delivery,the irrigation and aspiration of lens cortex and the timing of the operation were discussed.

Objective　To analyze the surgical results of uveitis-complicated cataract.Methods　Cataract phacoemulsification surgery was performed on 31 eyes (29 patients) due to uveitis (iridocyclitis 14 eyes, Fuchs heterochromic cyclitis 7 eyes, intermediate uveitis 7 eyes, Vogt-koyanagi-harada syndrome 2 eyes, and sympathetic ophthalmia 1 eye). Follow-up period was 5～28 months, averaging 15 months. Retrospective analysis was made.Results　Postoperative visual acuity≥0.5 was 80%. One eye (Vogt-koyanagi-harada syndrome) showed severe anterior uveitic reaction; One eye (iridocyclitis) had fibrinous membranes on IOL, and white debris on IOL were found in 2 eyes (intermediate uveitis).Conclusion　By using less traumatic phacoemulsification technique to ocular tissue, taking meticulous care to avoid stimulating iris, cleaning up cortex thoroughly during surgery and implanting the IOL in the bag, less inflammatory reaction, less complication and better vision acuity postoperatively at relative quiescence period of uveitis can be achieved.

Objective:To improve the safety,rationality and efficacy of medication for ovarian cancer patients complicated with cirrhosis by the participation of clinical pharmacists in the therapy. Methods:Clinical pharmacists participated in the therapy for an ovarian cancer patient with cirrhosis,and provided a rational and individualized therapeutic regimen through the drug experience of clinical pharmacists as well as the relevant medical guides and literatures. Results:The therapeutic efficacy was increased by the participation of clinical pharmacists in the therapy,the potential risk of the chemotherapy was avoided and the security of medication was assured. Conclusion:The participation of clinical pharmacists in therapeutic practice can improve the normalization of pharmacotherapy for ovarian cancer patients with cirrhosis,which also can provide ideas and methods for treating the similar patients.

Anemia, Hemolytic, Autoimmune ; therapy ; Antibodies, Monoclonal, Murine-Derived ; therapeutic use ; Female ; Humans ; Infant ; Rituximab

Aged ; Anthracosis ; microbiology ; Bacterial Proteins ; genetics ; China ; DNA Mutational Analysis ; DNA, Bacterial ; genetics ; DNA-Directed RNA Polymerases ; Drug Resistance, Bacterial ; genetics ; Humans ; L Forms ; genetics ; Middle Aged ; Mining ; Mutation ; Mycobacterium tuberculosis ; genetics ; isolation & purification ; Tuberculosis ; microbiology

Objective To observe the characteristics of morphosis parameter of the optic disc of physiologie large cup. Methods 100 eyes with physiologic large cup and 74 eyes with normal cup were examined by Heidelberg Retina Tomograph(HRT).The differences of morphosis parameters between two groups were analyzed comparatively on disc area(DA),cup volume(CV),cup/disc area ratio(C/DR),rim area(RA),cup volume(CV),rim volume(RV),mean cup depth(MeCD),maximum cup depth (MxCD),cup shape measure(CSM),height variation contour(HVC),mean retinal nerve fiber layer thickness(mRNFLt),and retinal nerve fiber layer cross-section area(RNFLcsa).The characteristics of the inferior.superior,nasal,and temporal quadrants of the physiologic large cups were analyzed.Results DA,CA,C/DR,CV,MeCD,CSM(P=0.00.respectively)and MxCD(P=0.04)were significantly larger in eyes with physiologic large cup than in eyes with normal cup.RA,RV,HVC,mRNFLt,RNFLcsa(P=0.00,respectively)were significantly smaller in eyes with physiologic large cup than in eyes with normal cup.The temporal quadrant of RV of the physiologic large cup is the narrowest.RNFLcsa decreased as the following order:superior,inferior,nasal,and temporal(P＜0.05).Conclusions Morphosis parameter of the optic discs of physiologic large cup has its own representation on characteristics.Compared to normal cups,physiologic large cups had larger discs but smaller mRNFLt.The nasal quadrant of DA was larger than the inferior quadrant.

OBJECTIVE To discuss the occupational hazard factors and protection measures of Central Supply Unit.METHODS The occupational hazard factors in the Central Supply Unit was analyzed and some suggestion on the occupational protections to decrease occupational risk was done.RESULTS It is necessary to give some special training about the occupational safety,especially through various sharp instrument wound-treatments to establish some protection and countermeasures CONCLUSIONS The pertinent occupational protection strategy according to the actual hazard factors is more efficient and more benefit for the high quality management of Central Supply Unit which will enhance the standardization,quality and working efficiency,and ensure the work safety and quality.

Objective To investigate the change of splenocyte subsets in Balb/c mice immunized with transgenic alfalfa(Medicago sativa)containing Eg95-EgA31 fusion gene of Echinococcus granulosus(Eg) and challenged with Eg protoscoleces.Methods Leaf protein was extracted from transgenic alfalfa containing Eg95-EgA31 fusion gene by heat-coagulation method,and concentration of 20 g/L was used in the study.Meanwhile,leaf protein extracted from the transgenic alfalfa containing blank vector(pBI121)and the normal alfalfa was served as control.Thirty-two female Balb/c mice were randomly divided into 4 groups,8 mice in each group.Oral group was immunized with the leaf protein containing Eg95-EgA31 fusion antigen intragastrically(100μl per mouse);intranasal group was immunized with the leaf protein containing Eg95-EgA31 fusion antigen intranasally(10 μl per mouse);blank vector group was vaccinated intranasally with 10μl leaf protein with blank vector(pBI121);and normal control group was given 100μl normal leaf protein intragastrically.All mice in the above mentioned groups were immunized every 3 days for 2 months.Then,the mice were challenged intraperitoneally with Eg protoscoleces(50 protoscoleces per mouse)8 weeks after last vaccination and sacrified 24 weeks pest infection to separate the splenocytes.Flow cytometry was used to measure the percentages of CD4+ and CD8+ T ceils subsets.Resuits Compared with the normal control group(0.166±0.018,0.083±0.006,2.019 ±0.369),the percentages of CD4+(0.286±0.009)and CD8+(0.102±0.004)T cell subsets and the ratio of CD4+/CD8+(2.814±0.014)in oral group increased significantly (P<0.01 or<0.05).The percentage of CD4+ subset(0.269±0.016)and the ratio of CD4+/CD8+(2.955±0.986) in intranasal group was significantly higher than that ofthe normal control group(all P<0.01).The percentage of CD4+ subset in oral group was significantly higher than that of the intranasal group(P<0.05).No significant difference was found in the percentages of CD4+ and CD8+ T cell subsets and the ratio of CD4+/CD8+ between the blank vector group(0.169±0.018,0.093±0.019,1.852±0.188)and the normal control group(all P>0.05).Conclusions CD4+ T cell may play an important role in the protection induced by transgenic alfalfa vaccine against the challenge of Eg protoscoleces.Intragastrical immunization may be a good route.

Objective To explore the value of serum 1,5-AG in the diagnosis of diabetes mellitus by applying the method of pyranose oxidase (PROD). Methods 1,5-AG,FPG、2hPG and HbA_1c were determined in 50 case of DM,100 case of IGT and 30 control subject. ROC curve was applied to evaluate the diagnostic efficiency of 1,5-AG,FPG and HbA_1c for DM. Results Serum 1,5-AG was obviously lower in the diabetes than in IGT and controls(P＜0.01). Compared with controls, IGT patients had lower serum 1,5-AG(P＜0.05).1,5-AG level had significantly negative correlation with FPG,2hPG and HbA_1c respectively. The area under the ROC curve of 1,5-AG,FPG and HbA_1c are 0.948、0.921、0.817,respectively. Conclusions 1,5-AG level shows significantly correlation with glucose tolerance. Serum 1,5-AG measurement could be used as useful indicator in the screening of diabetes mellitus as well as in the monitoring of glycemic control.

Objective To observe the results of function MRI and perimetry in patients with visual pathway diseases. Methods Three patients (6 eyes) with pituitary adenoma and craniopharyngioma diagnosed via pathological examination and three healthy volunteers aged from 24 to 30 were collected. The best corrected visual acuity was non-light perception-1. 0 in the 6 sick eyes and 1. 0 in the healthy eyes;all the involved individuals had no other ocular diseases except myopia and without any contraindications of MRI. Common tests including the best visual acuity, fundus test by direct or indirect ophthalmoscope, center static visual field tested by Octopus 101 perimeter, program 32, tendency oriented perimetry were performed. The visual stimulation subtended a field of view of about 12 degrees,consisted of high contrast and drifting checkerboards. MRI parameters: GE signa VH/i 3. 0T scanner. Functional data: GRE-EPI sequence, 20 slices lying perpendicular to the calcarine sulcus. Anatomical data was obtained using 3DSPGR sequence to acquire high resolution. The cortical surface was unfolded and then cut and inflated. Functional data was presented to the inflated surface and subsequently analyzed by AFNI software. Results In six eyes, three had temporal defects, two had upper temporal visual field defects, and the other one did not finish the visual field test. The retinotopic representations of health adults were obtained by using the phase-encoded visual stimulation. The Eccentricity coordinate maps showed that foveal representations lay in the occipital poles and the representations appeared further anterior as eccentricity increased. The polar angle coordinate maps showed that early retinotopically organized areas had a representation of visual field. The visual cortex beneath the calcarine sulcus matched with the upper visual field of the opposite side and which upon the calcarine sulcus matched with the under visual field of the opposite side. Less or no visual cortex response was revealed in the patients' function MRI or the response in injury side was vanished. The visual cortex response related with the visual field defects could not be induced in function MRI. Conclusion There is a good correlation between function MRI data and the results of perimetric evaluation. The function MRI can show the visual cortex response correlated with the visual field defects of the patients with visual pathway diseases.