Neuroblastoma (NB)is a common malignant solid tumor in childhood,which has various clinical manifestations.It is difficult to diagnose and to prone to early metastasis.It is one of the main diseases causing death in childhood.The treatment of children with high-risk NB is still very challenging.At present,the treatments of NB mainly concentrated in the surgery,chemotherapy,radiation therapy and transplantation technology,etc.In recent years,more studies focus on targeted therapy and molecular biology (genetics) treatment.Targeted therapy is targeted to different specific loci of tumor,so as to kill tumor cells and reduce the impact on the normal tissue,which supplies more safe and effective treatments for children with NB.

Objectives: To investigate the effects of the extract mixture of alfalfa and perilla on lowering serum lipid, protecting liver and aortic wall in rats. Methods: Sixty SD rats were divided into six groups and were fed different diet. After seven or fourteen days, the rats were put to death and measured for serum lipids, cholesterol content of liver and aortic wall, and pathological change of the tissue. Results: There was a significant increase of serum TC, LDL-C, cholesterol content of liver and aortic wall in the high-fat fed rats. Under light microscope, there were lipid droplets and cholesterol crystal in liver of group high-fat. Compared with the group high-fat, the rats had a significant decrease of those measurements in serum or in tissue in the alfalfa and perilla group. Conclusions: The extract mixture of alfalfa and perilla could prevent the increase of serum TC, LDL-C, cholesterol content of liver and aortic wall in high-fat fed rats, and also prevent the fatty infiltration and cholesterol deposit of liver.

Five inner ears were removed from five rabbits under aneasthesia. The hard, thick bones of outer and middle ears were trimmed off, and the auditory ossicles were taken away. The cochleae were fixed with 2％ glutaraldehyde in phosphate buffer for eight hours, then they were decalcified in 10％ EDTA-2Na solution for ten days, and the softened shells of the cochleae were stripped off, and post-fixed in 1％ OsO_4 for an hour, dried at critical point, coated with gold, and observed under SEM.The rabbit cochlea shows two and a half turns, the modiolus is about 3.5mm high, and the organ of Corti lies on the basilar membrane of the spiral lamina. The outer hair cells usually form three parallel rows on the middle and basal turns but occasionally four rows on the top half turn. The inner hair cells form only a single row. Every bunch of the outer hairs consists of about 93-114 stereocilia which together show a "W" shape. Every bunch of inner hairs has about 50 cilia which together show an arch shape. The cilia on the top turn are longer than those on the base. The length and width of the inner and outer hair cells and four other kinds of cells were measured according to photos, and the numbers of these kinds of cells were estimated.Other three cochleae fixed at the time of 1, 2 or 4 hours after death were also observed. In the cochlea fixed four hours after death, the majority of the auditory hairs were found fallen down, which means the fixation of cochlea should be carried out within less than four hours after death.Some of the structural similarities and differences of cochleae among rabbit,human being and guinea pig were also compared in this paper.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Lymphatic Diseases ; etiology ; pathology ; Male

Dental Implantation ; standards ; Humans ; Terminology as Topic

OBJECTIVETo observe treatment efficacy of acupuncture at different distal acupoints for acute lumbar sprain after massage.

METHODSOne hundred and fifty patients with acute lumbar sprain were randomly divided into a Yaotongdian (Extra) group, a Houxi (SI 3) group, a Weizhong (BL 40) group, a Chengshan (BL 57) group and a Shuigou (GV 26) group, total 5 groups, 30 cases in each one. After the same massage treatment and based on groups divided, different distal acupoints above-mentioned were selected to be acupunctured. Visual analogue scale (VAS) scores, lumbar activity and treatment efficacy of patients in 5 groups were evaluated.

RESULTSWith acupuncture at distal acupoints after massage,VAS scores of patients in 5 groups were decreased compared with those after massage treatment (all P<0.05); lumbar activity was all obviously improved (all P<0.01); and cured and markedly effective rates were all increased in comparison with those after massage (all P<0.01). But among 5 groups the differences of VAS scores, lumbar activity and cured and markedly effective rates were not statistically significant(all P>0.05).

CONCLUSIONBased on massage treatment, acupuncture at distal acupoints could further improve the treatment efficacy for acute lumbar sprain, but there is no obvious effective difference among every distal acupoint. As long as choosing acupoints with lower pain threshold and stimulating enough, good efficacy could be acquired for acute lumbar sprain.

Acupuncture Points ; Acupuncture Therapy ; Acute Disease ; therapy ; Adolescent ; Adult ; Aged ; Combined Modality Therapy ; Female ; Humans ; Lumbosacral Region ; injuries ; Male ; Massage ; Middle Aged ; Sprains and Strains ; therapy ; Treatment Outcome ; Young Adult

Objective To explore the efficacy and safety of plastic titanic plates internal fixation in the treatment of intra-articular calcaneus fracture.Methods 42 patients with intra-articular calcaneus fracture were selected and taken lateral calcaneal improvement “L” shaped incision for the correction of calcaneal shortening and valgus deformity.Skidmore needle went through collapse of the articular surface below from calcaneal fractures after block nodules along with bone shaft,and restored Bohler angle,Gissane angle and bone length,height,and the lateral fracture piece was reset.The fixation was taken by plastic reconstruction titanic plates,and the fracture was inspected by Xray.Results In 42 patients,the fracture healing time was (11.4 ± 0.6) weeks.The fracture position was good on the line,and the articular surface of calcaneus and talus was smooth,and the Bohler angle,Gissane angle of calcaneus were returned to normal.The excellent rate was 90.5％ by Maryland score.No surgical causes of broken plate and screw breakage were found.Conclusion Internal fixation with open reduction and plastic titanic plates internal fixation for the treatment of intra-articular calcaneal fractures has a significant effect and safety,which is worthy of promotion.

To promote the development of key discipline construction in hospitals,we described the integration of refined management into the construction of key disciplines and different aspects including personnel training,featured specialty,research,and hardware were discussed.We concluded that refined management in essential to the development of hospital,and a mechanism needs to be established to ensure the long-term effect.

BACKGROUND:Conventional treatments for hypertrophic scars include excision, steroid hormones, anti-metabolite drugs, immunosuppressive agents and radiation therapy. Easy to relapse or serious reaction limits their clinical use. In recent years, application of calcium channel blockers in treatment of hypertrophic scars has made more good progresses, but little adverse reactions are obtained. OBJECTIVE:To explore the effects of calcium channel blocker trifluoperazine on hypertrophic scar of rabbit ears. METHODS:A total of 24 rabbits were enrol ed in this study. After 1 week of accommodation, models of rabbit ear scar were established in accordance with the method of Morris and Li et al. Rabbit models were randomly assigned to three group (n=8). At 30 days after model induction, when scar formed, trifluoperazine and triamcinolone acetonide groups received trifluoperazine and triamcinolone acetonide injection. Blank control group was left intact. Changes in hyperplastic scar, hypertrophic index, levels of matrix metal oproteinase-2, tissue inhibitor of metal oproteinase-2, transforming growth factorβ1,α-smooth muscle actin and proliferating cellnuclear antigen were compared and observed in each group. RESULTS AND CONCLUSION:At 10 and 20 days after treatment, in the three groups, skin bulge was visible in rabbit ears and no rabbit hair grew. Rabbit ears had obvious softening in the trifluoperazine group compared with the triamcinolone acetonide group, showing dark red. In the blank control group, rabbit ear scar was evident and showed red color. At 20 days after treatment, scar thickness and scar index were lower in the trifluoperazine and triamcinolone acetonide groups than in the blank control group. Matrix metal oproteinase 2 expression was significantly higher, but tissue inhibitor of metal oproteinase-2 and transforming growth factorβ1 levels were lower in the trifluoperazine and triamcinolone acetonide groups than in the blank control group. Results indicated that trifluoperazine obtained good proliferative effects on rabbit ear scar, and could decrease scar thickness.

Objective To observe the changes in help T cell 1/2 cytokines and transcription factors in murine cytomegalovirus (MCMV) infected newborn mice and to determine the effectiveness of intervention with thymopentin.Methods One hundred and twenty newborn BALB/c mice were randomized into a blank group,model group and thymopentin group,with 40 mice in each group.Mice in the model and thymopentin group received an intraperitoneal injection of MCMV suspension within 4-6 hours after birth at the dosage of 20 μ L to and tissue culture infections dose of 10431 U/0.1 ml,establish a systemic infection model,and the same volume of normal saline was injected into mice in the control group.Mice in the thymopentin group also received thymopentin 0.3 mg/(kg · d).On Day 3,5,7,10 and 14,8 mice in each group were sacrificed and their splenic tissues were harvested.Interferon (IFN) γ and interleukin (IL)-10 expression in the supernatant of a splenic lymphocyte culture was assessed by enzyme-linked immunosorbent assay.T-bet/GATA-3 mRNA in splenic tissues were analyzed using reverse transcription-polymerase chain reaction.One way ANOVA,including LSD and Dunnett T3 methods,was used for statistical analysis.Results (1) IFN-γ expression in the model and thymopentin groups peaked on Day 3,and was higher than that in the control group [(280.73 ± 14.88),(286.03 ± 15.44) and (149.42±5.43) pg/ml,respectively,F=183.532,P=0.000].Expression in the thymopentin group decreased after Day 3,and increased by Day 7.At Day 14,the IFN-γ expression level in the thymopentin group was higher than that in the control and model groups [(252.33±8.33),(149.07±7.05) and (148.57±4.53) pg/ml,respectively,F=385.487,P=0.000].(2) IL-10 expression in the model group gradually increased.By Day 14,the expression became obviously higher than that in the control and thymopentin groups [(71.19± 1.50),(36.67 ± 2.55) and (40.01 ± 1.28) pg/ml,respectively,F=523.670,P=0.000].IL-10 expression in the thymopentin group increased after Day 3 and decreased by Day 7.On Day 14,the expression in the thymopentin group was lower than that in the model group,but higher than that in the control group.(3) T-bet mRNA expression was obviously increased in the model and thymopentin groups.On Day 3,the expression was higher than that in the control group (relative value of gray-scale:0.74±0.02,0.71 ±0.04 and 0.30±0.01,respectively,F=741.630,P=0.000).By Day 3,the expression in the thymopentin group decreased,and gradually recovered on Day 5,and on Day 14 it was higher than that in the control and model groups (relative value of gray-scale:0.45 ± 0.01,0.30±0.01 and 0.30±0.01,respectively,F=257.571,P=0.000).(4) GATA-3 mRNA expression in the model and thymopentin groups increased on Day 3,and was higher than that in the control group (relative value of gray scale:0.48±0.02,0.53±0.01 and 0.33±0.01,respectively,F=345.167,P=0.000).On Day 14,the expression in the model group was higher than that in the thymopentin group,which was higher than that in the control group (relative value of gray-scale:0.99 ± 0.02,0.55 ± 0.02 and 0.34 ± 0.01,respectively,F=1 767.505,P=0.000).Conclusions A Th1/Th2 shift may be induced in MCMV infected neonatal mice,which manifests as a state of predominant Th2 response.Thymopentin can ameliorate this situation.