Objective To report two childhood acute myeloid leukemia (AML) patients with t(8;20)(q22;q13) and t(1;8;21)(q32;q22;q22) respectively,as variant t(8；21).Methods Chromosome preparation of bone marrow cells were made using short-term culture and karyotypic analysis was carried out using R and G-banding techniques.Interphase-fluorescence in situ hybridization (I-FISH) and metaphase-FISH (M-FISH) were performed using dual color,dual fusion AML1-ETO probe to detect the AML1-ETO fusion gene.Multiplex RT-PCR was used to demonstrate the expression of AML1-ETO fusion transcript.Results The karyotype of bone marrow cells for these two childhood AML patients were 45,X,-Y,t(8;20)(q22;q13)[12]/46,XY[3](case 1) and 46,XX,t(1;8;21)(q32；q22;q22)[18]/46,XX[2](case 2),respectively.I-FISH and M-FISH confirmed that they all had the AML1-ETO fusion gene and variant t(8;21).The AML1-ETO fusion transcript in both patients was detected by RT-PCR.Conclusion t (8;20)(q22;q13) and t (1;8;21)(q32;q22;q22) are variant t (8;21) in nature.It is important to combine the conventional karyotypic analysis with D-FISH and multiplex RT-PCR to determine the nature and prognosis of AML patients with variant t(8;21).

Objective To investigate the influence of preoperative location of modified loop ileostomy with single incision on the complications and nursing of low rectal cancer patients with la-paroscopic radical resection of anus preservation.Methods The location of 78 patients with modi-fied preventive ileostomy with single incision was 2~3 cm above the McBurney point with 15~20° angular inside.Results There was one case with stoma hernias,three cases with stoma dermati-tis,four cases with stoma infection,more than 80% of the patients had no discomfort or had mild discomfort.Conclusion Correct preoperative location before stoma is easy to nurse and could re-duce the occurrence of stoma complications.

Objective To investigate the influence of preoperative location of modified loop ileostomy with single incision on the complications and nursing of low rectal cancer patients with la-paroscopic radical resection of anus preservation.Methods The location of 78 patients with modi-fied preventive ileostomy with single incision was 2~3 cm above the McBurney point with 15~20° angular inside.Results There was one case with stoma hernias,three cases with stoma dermati-tis,four cases with stoma infection,more than 80% of the patients had no discomfort or had mild discomfort.Conclusion Correct preoperative location before stoma is easy to nurse and could re-duce the occurrence of stoma complications.