Coronary heart disease(CHD) is the leading cause of death among women worldwide.Recent technological advances in cardiovascular diagnosis and treatment have not led to significant declines in mortality for women with CHD when compared to the dramatic declines for men.Owing to the gender differences of physiology and social circumstances,women with CHD may have different manifestations as compared to men with CHD.Recently,research has shown that women with CHD had mainly the following characteristics:special risk factors,atypical ischemia symptoms,multiple risk factors and comorbidities at initial physician consultation,higher under-treatment rate and higher mortality rate.At the same time,most CHD researches have paid little attention to women.Therefore,the current CHD guidelines may not be completely suitable for women.In order to improve the prevention and treatment of CHD in women,we advocate to enhance research and clinical work for women with CHD. Abstract:Summ ary:Coronary heart d isease(CHD) is the lead ing cause of death among wom en worldw ide.Recent technological advances in card iovascu lar d iagnosis and treatm ent have not led to sign ificant dec lines in mortality for wom en w ith CHD when compared to the dram atic dec lines form en.Ow ing to the gender d ifferences of physiology and soc ial c ircum stances,wom en w ith CHD m ay have d ifferent m an ifestations as compared to m en w ith CHD.Recently,research has shown that wom en w ith CHD had m ain ly the follow ing characteristics: spec ial risk factors,atyp ical ischem ia symptom s,mu ltip le risk factors and comorb id ities at in itial physic ian consu ltation,h igher under-treatm ent rate and h igher mortality rate.At the sam e tim e,most CHD researches have paid little attention to wom en.Therefore,the current CHD gu idelines m ay not be comp letely su itab le forwom en.In order to improve the prevention and treatm ent of CHD in wom en,we advocate to enhance research and c lin ical work for wom en w ith CHD.

Objective: To investigate whether FasL expression by exogenous FasL gene transfer had regulatory effect on drug sensitivity of tumor cells. Methods: Exogenous Fasl, gene transfer was mediated by lipofectAMINE. The FasL expression was detected bv Immunohistochemistry and RT-PCR. The growth inhibition of cytotoxic drugs on tumor cells was measured by MTT assay. Fas mRNA expression was analyzed bv Semi-quantitative RT-PCR. Results: The eukaryotic expression vector, PcDNA3-FasL and PcDNA3 were successfully transteeted into MCF-7 cells. The transfectant cells was named as MCF-7/FL or MCF-7/Vt respectively. FasL was expressed on MCF-7/FL but not on MCF-7/Vt or MCF-7. The cytolysis ot MCF-7/FL induced by adriamycin at concentration of 0.625 ~ 2.5 ?g/ml, cisplatin of 1 .25 ~ 10 ?g/ml, or methotrexate of 3 ~ 6 ?g/ml respectively were all significantly enhanced compared with the cytolysis of MCF-7. Blocking FasL by using F(ab' )2-anti APO-1 antibody markedly reduced this up-regulated cytolysis induced by these drugs The significant up-regulation of Fas mRNA on MCF-7/FL was observed by Semi-quantitative RT-PCR after these drugs treatment. Conclusion: These findings indicated that FasL expression by gene transfer had up-regulation effect on drug sensitivity of tumor cells, this effect was associated with enhanced Fas expression induced by cytotoxic drugs.

Ascending and descending efferent projections of superior coUiculus were indentified by means of the anterograde horseradish peroxidase technique in the cat.Following injections of HRP into the rostral superior colliculus, a large number of labeled terminals were found mainly in several of the ipsilateral thalamic nuclei:ventromedial division of the ventral nucleus of the lateral geniculate body, zona incerta,lateral posterior nucleus, parafasciculus nucleus and centromedian nucleus. The mediodorsal nucleus and thalamic reticular nucleus were also found mildly labeled.Labeling were most heavily concentrated in the ipsilateral dorsolateral division of pontine nucleus and caudal region of parabigeminal nucleus. The dorsal part of medial accessory olivary was the only nucleus labeled contralaterally. After injecting HRP into the caudal superior colliculus, only the dorsolateral division of pontine nucleus was densely labeled, while labeled terminals of caudal parabigeminal nucleus signifcantly reduced in amount. No labeled terminals were found in the thalamus as well as medial accessory olivary nucleus. These results indicate that the efferent connections of superior colliculus are topographically organized.

The different distributions of substance P(SP)-and leucine-enkephalin (L-ENK)-like immunoreactive neurons in the midbrain raphe nuclei of the rats have been studied, using PAP immunocytochemical technique. The results showed four main points: 1. SP-containing immunoreactive cell bodies were only seen in the most caudal part of the dorsal raphe nucleus, the average number of SP-positive cell bodies was about 167 in each animal. The L-ENK-like immunoreactive positive cell bodies were found almost in the whole length of the dorsal raphe nucleus, the L-ENK-positive cell bodies were about 132 in each animal. 2. L-ENK-Iike immunoreactive perikarya were mainly located in the midline area of the dorsal raphe nucleus. Besides the above areas, SP-containing immunoreactive cell bodies were also located bilaterally in the extended area from the raphe. 3. L-ENK-containing immunoreactive cell bodies constantly appeared in the median raphe nuclei, the average number was about 199 in each animal, but SP-containing immunoreactive bodies were only observed in this nucleus of two rats. 4. Some SP-like immunoreactive cell bodies or processes were observed in the vincinty of vascular walls, but the L-ENK positive neurons were not found similar results. The present experiment showed that the SP-like immunoreactive perikarya is located in the median raphe nuclei of the rat.

Objective To evaluate the diagnosis value of urodynamics in patients with benign prostate hypertrophy(BPH).Method With urodynamic device,the full set of urodynamic exam was administrated in 427 patients with BPH,and the externalsphincter urethral myogram was monitored simultaneously in pressure-flow studies(PFS).The umdynamic finding such as Q_(max),P_(det)-Q_(max),DS(descending slope)and post-voiding residual(PVR)were recorded,as well as the situation of bladder detrusor constraction and bladder compliance and urethral sphincter coodination.The bladder outflow obstruction was diagnosed by A-G nomogram,P-Q plot and DS.The IPSS score and prostate volume were also acquired.Results The diagnostic rate of BOO is 81.5%,among them concomitantly detrusor muscle impair in 117 cases (27.4%),decreased bladder compliance in 162 case(37.9%),urethral sphincter dyssynergia in 148 cases(34.7%),and unstable bladder in 164 cases(38.4%).The increase degree of BOO show an increasing tendency with urodynamic finding such as Q_(max),P_(det)-Q_(max),P_(open),DS,IPSS score and prostatic volume respectively,but a decreasing tendency with Q_(max) and bladder compliance.Conclusions The urodynamic exam plays an important role in diagnosis of BOO.There is a positive relation among degree of BOO with urodynamic findings such as P_(det)-Q_(max),P_(open),DS and IPSS score and prostatic volume,however,a negative relation with Qmax and bladder compliance respectively.

Objectives To explore status of health prevention knowledge awareness and the formation of healthy behaviors of community residents in Guagzhou and provide evidence for health-management-related departments to put forward pertinent intervention strategies. Methods A questionnaire-based survey was conducted among 2 790 community residents in Guangzhou by multistage stratified random sampling. Results The awareness rate of residents′ basic health prevention knowledge was 78.14%, the awareness rate of key knowledge was 74.77%, the rate of health behavior formation was 48.14%. The rate of women′s behavior formation was higher than that of men′s. The awareness rate of basic knowledge among the residents aged over 50 years was lower than that that among those aged less than 50 years , but the awareness rate of key knowledge and behavior formation rate were higher. The level of knowledge awareness and the rate of behavior formation rate of urban residents were higher than those of rural residents and migrants. The knowledge awareness and behavior formationi were positively correlated with degree with statistically significant difference (P < 0.01). Conclusions The health belief and behavior of the community residents should be cultivated and strengthened , the basic prevention knowledge should be made universal for middle-aged and old people , the health education of key prevention knowledge should be strengthened for the middle-aged and young people , the public health services should be made more open and equal further promoted, and the community comprehensive intervention focusing on the health education should be strengthened.

Objective To investigate the clinical efficacy and related factors of holmium laser lithotripsy under ureteroscopy in treating mid -upper ureteral calculi.Methods The clinical data of 65 patients with mid -up-per ureteral calculi were retrospectively analyzed.Results The overall stone -free rate was 90.7% (59 /65 ). 4 weeks after operation,the stone -free rate was 94.9%(56 /59).The incidence rate of complications was 3.3%(2 /59).Stone size,hydronephrosis,ureteral stricture and twist,complicated with ureteral polypus were related to the success of lithotripsy(P =0.028).Conclusion Holmium laser lithotripsy under ureteroscopy in treating mid -upper ureteral calculi is safe and effective,improve surgical techniques and pay attention to cases screening may improve the success rate.

The topographical projection between the parabigeminal nucleus and the superiorcolliculus in the cat was investigated by retrograde and anterograde HRP tracingtechnique.Following HRP injection in the rostral superior colliculus,the labeledsomata were found predominantly in the rostral region of contralateral parabigeminalnucleus,but labeled terminals were heavily distributed in the ipsilateral parabigeminalnucleus,mainly in the caudal region.Following injection of HRP into caudal superiorcolliculus,the labeled somata and terminals were seen in ipsilateral parabigeminalnucleus,chiefly in caudal part,but their number was significantly decreased.It wasnoted that the labeled somata were sparse but labeled terminals were not found inthe parabigeminal nucleus,when the injection was restricted to superficial layers ofsuperior colliculus.After HRP injection into deeper layers of superior colliculus,there existed not only the labeled somata but also the labeled terminals in theparabigeminal nucleus,the former was distributed in bilateral parabigeminal nucleus,the latter in ipsilateral nucleus,chiefly in the caudal part.These results show thatthere are topographical connections between the parabigeminal nucleus and superiorcolliculus,and the parental somata contributing axons to the parabigeminal nucleusare located in deeper layers of the ipsilateral superior colliculus.

OBJECTIVE:To investigate the improvement effects of Zhuanggushenjin capsules on osteoporosis induced by reti-noic acid in mice. METHODS:84 mice were randomly divided into normal control group,model control group,positive control group [Gushukang granules,5 g(crude drug)/kg] and Zhuanggushenjin capsules high-dose,medium-dose and low-dose groups [7.80,3.90,1.95 g(crude drug)/kg]. Except for normal control group,those groups were given retinoic acid(90 mg/kg)to induce osteoporosis model. 7 days after modeling,all drug groups were given relevant medicine intragastrically,normal control group and model control group given normal saline intragastrically ,once a day,for consecutive 14 days. ALP activity and the contents of cal-cium and phosphorus in serum and thigh-bone were determined. The body weight, the dry weight, length and diameter of thigh-bone were determined,and organ coefficients (spleen,testicle,ovarium and uterus) were calculated. RESULTS:Compared with normal control group,the activity of ALP in serum of mice increased in model control group,while the contents of calcium and phosphorus,body weight,length and diameter of thigh-bone decreased significantly;spleen coefficients increased,while testi-cle and uterus coefficients decreased(P<0.05 or P<0.01). Compared with model control group,the contents of calcium and phos-phorus increased in Zhuanggushenjin capsules groups,while spleen coefficient decreased;the activity of ALP in serum of mice de-creased in high-dose and medium-dose groups,while body weight,the length of thigh-bone,testicle and uterus coefficients in-creased;diameter of thigh-bone increased in high-dose group (P<0.05 or P<0.01). CONCLUSIONS:Zhuanggushenjin capsules can improve retinoic acid-induced osteoporosis in mice to certain extent.