1.Complementary Medicine & Mucosal Immunology-Recent Topics around Inflammatory Bowel Disease
Toshinori ITO ; Yasuyuki KAI ; Takumi IGURA ; Seiichi NAKAJIMA ; Toshiro NISHIDA ; Tsunekazu MIZUSHIMA ; Riichiro NEZU
Japanese Journal of Complementary and Alternative Medicine 2008;5(2):85-101
Complementary medicine aimed at improving patients’ QOL by complementing modern medicine has recently become the focus of attention. These alternative supplements or functional foods are commonly biologically based. Contained within food itself, these specialized components serve a tertiary function regarding biological regulation and defense. Further, in vivo mechanisms are considered to be closely linked to mucosal immunity of the intestine. As the mechanism of innate immunity is further elucidated, the significant role of certain food components in relation to mucosal immunity of the intestine has become a focus of interest.
In this paper, I would like to describe the experimental and clinical applications of complementary medicine in cases of chronic and/or intractable inflammatory bowel disease.
2.Induction of apoptotic lesions in liver and lymphoid tissues and modulation of cytokine mRNA expression by acute exposure to deoxynivalenol in piglets.
Osamu MIKAMI ; Hiroyuki YAMAGUCHI ; Hideo MURATA ; Yasuyuki NAKAJIMA ; Shigeru MIYAZAKI
Journal of Veterinary Science 2010;11(2):107-113
Six 1-month-old piglets were intravenously injected with deoxynivalenol (DON) at the concentration of 1 mg/kg body weight, with three pigs each necropsied at 6 and 24 h post-injection (PI) for investigation of hepatotoxicity and immunotoxicity with special attention to apoptotic changes and cytokine mRNA expression. Histopathological examination of the DON-injected pigs revealed systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and immunohistochemical staining against single-stranded DNA and cleaved caspase-3. The number of TUNEL-positive cells in the thymus and Peyer's patches of the ileum was increased at 24 h PI compared to 6 h PI, but the peak was at 6 h PI in the liver. The mRNA expression of interleukin (IL)-1beta, IL-6, IL-18, and tumor necrosis factor (TNF)-alpha in the spleen, thymus and mesenteric lymph nodes were determined by semi-quantitative RT-PCR, and elevated expression of IL-1beta mRNA at 6 h PI and a decrease of IL-18 mRNA at 24 h PI were observed in the spleen. IL-1beta and IL-6 mRNA expressions increased significantly at 6 h PI in the thymus, but TNF-alpha decreased at 6 h PI in the mesenteric lymph nodes. These results show the apoptosis of hepatocytes suggesting the hepatotoxic potential of DON, in addition to an immunotoxic effect on the modulation of proinflammatory cytokine genes in lymphoid organs with extensive apoptosis of lymphocytes induced by acute exposure to DON in pigs.
Animals
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Apoptosis/*drug effects/immunology
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Cytokines/*biosynthesis/genetics
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Gene Expression Regulation/drug effects
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Histocytochemistry/veterinary
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In Situ Nick-End Labeling/veterinary
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Liver/*drug effects/immunology
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Lymphoid Tissue/*drug effects/immunology
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RNA, Messenger/*biosynthesis/genetics/immunology
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Reverse Transcriptase Polymerase Chain Reaction/veterinary
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Specific Pathogen-Free Organisms
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Swine/*immunology
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Trichothecenes/*toxicity
3.Impact of Stroke Severity and Vascular Risk Factors on Early Versus Late Anticoagulation in Patients With Stroke and Atrial Fibrillation
Masatoshi KOGA ; Mattia BRANCA ; Daniel STRBIAN ; Takeshi YOSHIMOTO ; Kanta TANAKA ; Sohei YOSHIMURA ; Yusuke YAKUSHIJI ; Shigeru FUJIMOTO ; Adhiyaman VEDAMURTHY ; Manju KRISHNAN ; Marjaana TIAINEN ; Jochen VEHOFF ; Gerli SIBOLT ; Kosuke MATSUZONO ; Caterina KULYK ; Silja RÄTY ; Peter SLADE ; Alexander SALERNO ; Dimitri HEMELSOET ; Thomas HORVATH ; Takenobu KUNIEDA ; Makoto NAKAJIMA ; Hisanao AKIYAMA ; Yasuyuki IGUCHI ; Manabu INOUE ; Masafumi IHARA ; Kazunori TOYODA ; David SEIFFGE ; Martina GOELDLIN ; Jesse DAWSON ; Urs FISCHER
Journal of Stroke 2025;27(2):284-288
4.Impact of Stroke Severity and Vascular Risk Factors on Early Versus Late Anticoagulation in Patients With Stroke and Atrial Fibrillation
Masatoshi KOGA ; Mattia BRANCA ; Daniel STRBIAN ; Takeshi YOSHIMOTO ; Kanta TANAKA ; Sohei YOSHIMURA ; Yusuke YAKUSHIJI ; Shigeru FUJIMOTO ; Adhiyaman VEDAMURTHY ; Manju KRISHNAN ; Marjaana TIAINEN ; Jochen VEHOFF ; Gerli SIBOLT ; Kosuke MATSUZONO ; Caterina KULYK ; Silja RÄTY ; Peter SLADE ; Alexander SALERNO ; Dimitri HEMELSOET ; Thomas HORVATH ; Takenobu KUNIEDA ; Makoto NAKAJIMA ; Hisanao AKIYAMA ; Yasuyuki IGUCHI ; Manabu INOUE ; Masafumi IHARA ; Kazunori TOYODA ; David SEIFFGE ; Martina GOELDLIN ; Jesse DAWSON ; Urs FISCHER
Journal of Stroke 2025;27(2):284-288
5.Impact of Stroke Severity and Vascular Risk Factors on Early Versus Late Anticoagulation in Patients With Stroke and Atrial Fibrillation
Masatoshi KOGA ; Mattia BRANCA ; Daniel STRBIAN ; Takeshi YOSHIMOTO ; Kanta TANAKA ; Sohei YOSHIMURA ; Yusuke YAKUSHIJI ; Shigeru FUJIMOTO ; Adhiyaman VEDAMURTHY ; Manju KRISHNAN ; Marjaana TIAINEN ; Jochen VEHOFF ; Gerli SIBOLT ; Kosuke MATSUZONO ; Caterina KULYK ; Silja RÄTY ; Peter SLADE ; Alexander SALERNO ; Dimitri HEMELSOET ; Thomas HORVATH ; Takenobu KUNIEDA ; Makoto NAKAJIMA ; Hisanao AKIYAMA ; Yasuyuki IGUCHI ; Manabu INOUE ; Masafumi IHARA ; Kazunori TOYODA ; David SEIFFGE ; Martina GOELDLIN ; Jesse DAWSON ; Urs FISCHER
Journal of Stroke 2025;27(2):284-288