This cross-sectional study examines the association between coping with past ankle sprain, and current physical activity levels and sedentary times. It included a total of 1,464 male soccer players, who participated in the 6th Senior Soccer Festival in Shizuoka in 2017. A self-administered questionnaire was conducted on the day of the festival and 567 subjects responded (38.7% response rate). Participants answered questions regarding the coping with past ankle sprain, such as whether they had first aid treatment (yes/no), the type of initial treatment facilities (6 categories), and their most frequented facilities (5 categories). Additionally, the International Physical Activity Questionnaire Short Form was used to obtain information on current physical activity level and sedentary time. We compared the mean values of physical activity levels and sedentary times with the first aid treatment, initial treatment facilities and most frequented facilities answers, respectively. The mean age was 64.7 years. Physical activity level was associated with first aid treatment (yes: 3860.3 Metabolic equivalents (METs)/min/week; no: 3082.0 METs/min/week) (p=0.033). Sedentary time was also associated with first aid treatment (yes: 265.4 min/day; no: 321.3 min/day) (p=0.014). Initial treatment facilities and most frequented facilities were not associated with physical activity levels or sedentary times. Our study showed that those who had received first aid treatment had high physical activity levels and low sedentary time.