Objective: Enrollment in graduate schools presents a useful opportunity for registered nurses to enhance their expertise in their nursing field and gain better opportunities to achieve their desired career advancements. This study investigates the predictors associated with registered nurses’ interest in enrolling in master’s programs of nursing graduate schools.Materials and Methods: For the predictors associated with interest in enrolling in master’s programs of nursing science, we evaluated items related to registered nurses’ perceptions of their work environments and their impressions regarding master’s programs in nursing. The analyzed subjects were 3,611 female registered nurses working in 30 hospitals in Mie prefecture, Japan. Multiple linear regression analyses were conducted to investigate registered nurses’ predictors of interest in enrolling in master’s programs of nursing graduate schools. We included the variables with Variance Inflation Factor (VIF) less than (<) 2 in the statistical model.Results: Full-time nurses were more interested in enrolling in master’s programs than part-time nurses. Registered nurses who felt that they could keep up with courses in graduate schools, that they would be able to acquire skills to contribute to society, and that their colleagues were competent, showed stronger interest in master’s programs. Registered nurses who were under the impression that graduate school teachers were dedicated to their students showed lower interest in master’s programs. Registered nurses who felt that their superiors were competent and that they were expected to perform well by physicians also showed lower interest in master’s programs.Conclusion: Predictors significantly associated with registered nurses’ interest in enrolling in master’s programs of nursing graduate schools were determined. Further studies are required to gain a more detailed understanding of the nurses’ attitudes investigated.