1.Sequence analysis of the 5'-flanking region of human Pax-5 gene exon1B
Maolin LIU ; Rahman MIZANUR ; Hirabayashi YASUHIKO
Chinese Journal of Immunology 1985;0(05):-
Abstract Objective:To better understand the regulatory mechanism of Pax-5 gene expression on B-cell differentation and development.Methods:The isolation and genomic colning of the 5'-flanking region of Pax-5 gene were carried out according to established protocols: molecu-lar cloning and subcloning The nucleotide sequences were determined by a double-stranded dideoxy sequencing method. Sequence analysis wasperformed on line programs. Results:Analysis of tha total sequence(6 671 bp) shows the proximal promoter include 3 CAT boxes, 1SP1 and 1Ebox. However, there was no consensus sequence for a TATA box in the 5'-flanking region. Putative regulatory sites of further upstream in thesequence revealed 6LMO2 COM, 5NFAT, 2LPOLYA-B, 3GATA1, 2AP4, 10MZF1, 1ets1B, 1GATA3, 1NKX-25, 2RORA1, 1LYF1, 2Ikaros2,2TCF11 , 1GATA-C and 1FREAC7. Conclusion: The 5'-flanking regionn of human Pax-5 gene exon1B could be involved in regulating the expres-sion of human Pax5 and B-cell differentiation and development.
2.Sequence analysis of 5'-flanking region of human pax-5 gene exon 1B.
Mao-Lin LIU ; Mizanur RAHMAN ; Yasuhiko HIRABAYASHI ; Takeshi SASAKI
Journal of Experimental Hematology 2002;10(2):100-103
Pax-5 gene is important transcription factor in B-lymphopoiesis and B-cell development. To understand the regulatory mechanism of pax-5 expression, the immediate 5'-flanking region (6 671 bp) of human pax-gene exon 1B was isolated and characterized. Analysis of the total sequence showed that the proximal promoter includes 3 CAT boxes, 1 SP1 and 1 E box. However, there was no consensus sequence for a TATA box in the 5'-flanking region. Putative regulatory sites of further upstream in the sequence revealed 6 LMO(2)COM, 5 NFAT, 2 LPOLYA-B, 3 GATA1, 2 AP4, 10 MZF1, 1 ETS1-B, 1 GATA3, 1 NKX25, 2 RORA1, 1 LYF1, 2 Ikaros2, 2 TCF11, 1 GATA-C and 1 FREAC7. Therefore, the 5'-flanking region of human pax-5 exon 1B could be involved in regulating the expression of human pax-5 and B-cell differentiation and development.
5' Flanking Region
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genetics
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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genetics
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DNA
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chemistry
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genetics
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DNA-Binding Proteins
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genetics
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Exons
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genetics
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Humans
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Molecular Sequence Data
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PAX5 Transcription Factor
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Sequence Analysis, DNA
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Transcription Factors
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genetics