Objectives: We studied the effects of the fish oil (FO) diet given to obese Zucker (fa/fa) fatty rats on the serum and liver lipids concentrations, serum oxidative stress and antioxidant levels using diacron-reactive oxygen metabolites (d-ROMs) test and biological antioxidant potential (BAP) test. Methods: The Zucker fatty rats of 5weeks male were fed with diet for 4 weeks. The experimental diets were used normal diet or high cholesterol diet (HC). The fatty rat were fed with diet containing 10% Lard, 10% FO, 10% Lard+HC, 10% FO+HC. It was measured the concentrations of serum and liver lipids, serum d-ROMs and BAP. The d-ROMs and BAP were measured using Free Radical Elective Evaluator. The liver tissue were stained with Hematoxylin-Eosin staining that was observed with light microscopyResults: Although there were no difference in the both d-ROMs and BAP value among the diet, the d-ROMs value was tend to decrease and the BAP value was tend to increase in the FO group. The concentration of liver total cholesterol and triglyceride were decreased in the FO+HC group compared to the HC group. The number of fat droplet in the liver tissue were decreased in the FO group.Conclusion: Since the fat droplets was decreased in the FO group, it can be considered that fat accumulation was reduced to relieve oxidative stress in vivo. Thus, fish oil is expected to inhibit the oxidative stress in fatty rats.

Objectives: We studied the effects of the apple polyphenol (AP) with added alcohol on the weight of accumulated fat and dyslipidemia in obese (fa/fa) Zucker rats. Methods: The Zucker fatty rats of 5weeks male were fed for 4 weeks with the experimental diets used high cholesterol diet with 10% alcohol. The fatty rats were divided Control group and AP group. The control group without apple polyphenol, AP group was that there apple polyphenol. It was measured the posterior abdominal wall fat and testicle peripheral fat weight, the concentrations of serum and liver lipids.Results: The posterior abdominal wall fat weight indicated a tendency to decrease in the AP group compared to the Control group. The concentrations of serum total cholesterol and triglyceride decreased in the AP group compared to the Control group. There was no different between each group with respect to the concentration of cholesterol and triglyceride in the liver. The alanine aminotransferase decreased in the AP group compared to the Control group.Conclusion: These results suggested that the alcohol diets containing the apple polyphenol appear to effectively decrease the concentration of serum lipids and the alanine aminotransferase. The apple polyphenol were expected to prevent dyslipidemia and fatty liver.

Objectives: We studied the effects of capsaicin (CP) with high fat diets and alcohol (Alc）on the weight of accumulated fat and oxidative stress in rats. Methods: The Sprague-Dawley rats of 4weeks male were fed for 4 weeks with the experimental diets using high fat diet. The rats were divided Control group, CP group, Alc group and CP+Alc group. Alcohol was used as a drinking solution instead of water. The control group was fed without capsaicin. It was measured fat accumulation and oxidative stress in rats. Results: There was no different between each group with respect to the body weigt gain and total foods intake. The posterior abdominal wall fat weight, concentration of liver triglyceride and oxidative stress degree of the CP group and the CP+Alc group were lower significantly than those of the Control group, and those of the CP+Alc group was lower significantly than thoes of the Alc group. Conclusion: These results suggested that the high fat diets and alcohol diets containing the capsaicin appear to effectively decrease the fat accumulation and oxidative stress. The capsaicin were expected to prevent dyslipidemia.

Objective: We studied the effect of linseed oil (LO) and perilla oil (PO) diets (rich in α-linoleic acid) compared to a normal diet in rats, and assessed liver lipid concentrations and oxidative stress. Methods: Male Sprague-Dawley rats aged 5 weeks in three groups were fed a normal diet for 20 days, and liver lipid concentrations and oxidative stress were then measured in each rat. The groups were divided into a normal diet containing either lard (the control group: n=6), the LO group (n=6) and the PO group (n=6). Results: Final body weight tended to be lower in the PO group than in the Control group. There was no difference in total food intake between each group. Liver lipid concentrations and oxidative stress were significantly lower in the LO and the PO groups than in the Control group. Conclusion: These results suggested that diets containing LO and PO caused decreased concentrations of liver cholesterol and oxidative stress in rats compared to the control diet. Linseed oil and perilla oil diets are shown to prevent dyslipidemia and to relieve oxidative stress in vivo.

Objectives: We examined the effect of oil intake differing in fatty acid composition on fat accumulation amount, concentration of serum and fat droplets adipocytes of liver tissue in rats. Methods: Young male rats were fed a high cholesterol diet containing either lard, soybean oil, perilla oil or fish oil for 4wk. The control diet contained a lard component. Results: Posterior abdominal wall fat and testicle peripheral fat weights were significantly lower in the fish oil group compared with the control and the soybean oil group. The number of fat droplets was significantly higher in the liver tissue of rats in the perilla oil group compared with the other groups, and many fat droplets with small diameters were observed. The proportion of fat droplets to the total area was significantly lower in the perilla oil group and the fish oil group compared with the control and the soybean oil group. Conclusion: The results suggested that oil intake differing in fatty acid composition correlates with fat accumulation amount and lipid droplet area in the liver tissue. Fish oil intake correlated with reduced visceral fat accumulation and is expected to be useful in the prevention and improvement of obesity.

The purpose of this study was to clarify the effects on oxidative stress and the kidney tissues of obese type2 diabetes model rats when catechins were ingested. 5-week-old obese type 2 diabetes model male rats were kept for 4 weeks. A normal diet was fed to a control group (n=6), while an experimental catechin group (n=6) was fed catechin. Posterior subcutaneous abdominal fat weight and oxidative stress were measured, and kidney tissues were observed. Posterior subcutaneous abdominal fat weight and oxidative stress were significantly lower in the catechin group than in the control group. In the kidney tissues, the mesangial region in the control group was larger than in the capillary space, and the glomerulus was lobulated. The mesangial area in the catechin group was almost equivalent to the capillary space. Ingestion of catechin in obese type 2 diabetes model rats reduced fat accumulation and oxidative stress. It has also been suggested that catechin suppresses damage to mesangial cells in kidney tissue. Catechin intake likely suppresses oxidative stress and diabetic nephropathy inflammation, and it could be useful for the prevention of diabetes.