Objective To explore the information distribution of PM2.5 periodical paper both in English and Chinese in China.Methods Comprehensive analysis result was made based on the overall output of literature,academic attention trend,literature distribution and growth trend,authority institution and author ranking using high volume database,data mining,clustering analysis and visualization technology.Results There has been a rapid rise in the Chinese PM2.5 periodical paper in recent 5 years,with a sharp rise during the year 2011 to 2013 and the biggest rise in 2012.The periodicals that carry Chinese PM2.5 literature are mainly in the subjects of environment,health,resources.Researching organizations are mainly domestic famous comprehensive universities or research institutions.Core journal lists include those of China science and technology information institute,Peking University,and Chinese academy of science.Research terms mainly include PM2.5 air pollution,haze,particulate matter.Compared to the PM2.5 literature in Chinese,there has been slower development of literature in English in recent 5 years.Research organizations mainly include famous comprehensive universities or research institutions,and most of the literature are embodied in SCI,EI and Medline.Conclusions Some certain achievements have been made towards the PM2.5 research in China and the overall academic level of periodical paper is high,laying solid foundation for having a scientific knowledge of PM2.5 and taking countermeasure for haze.

Purpose:To study the expression of CK19 and CK2 0mRNA in peripheral blood patients with FRGO Stage ⅠA to ⅡA cervical carcinoma and to investigate its clinical significance. Methods:Using the reverse transcription polymerase chain reacti on(RT-PCR),CK19 and CK20mRNA expression was examined in peripheral blood fro m 250 patients with early cervical carcinoma before operation,50 Patients with benign gynecological tumors and 18 healthy volunteers. In 250 patients,possible correlations between clinical pathological factors were analyzed. Results:The positive expression rates of CK19 and CK20mRNA were 36% and 24% in 250 cervical carcinomas respectively,in comparison with 3.0% an d 0% with benign gynecological tumors and all subjects in healthy volunteers wer e negative; The expression of CK19 and CK20 mRNA were significantly correlated w ith lymph vascular space involvement,but was not associated with prognostic fac tors including stage,differentiation,pathological types ,lymph node metastasis ,bully tumor size . In patients with CK19 mRNA(+)/CK20 mRNA(+),the rate of lymp h node metastasis and vascular space involvement and recurrence outside the pelv is was significantly higher than that of patients with CK1R mNA9(-)/CK20 mRNA(- )(P

Background and purpose:To evaluate the treatment regimens used for patients with yolk sac tumors of the ovary and prognostic factors in our hospital. Methods:Clinical data for the patients with yolk sac tumors of the ovary were analyzed retrospectively. Patients were divided into four groups: patients in A group received nonsparing surgery and platinum-based chemotherapy; in B group received nonsparing surgery and non-platinum-based chemotherapy; in C group received fertility-sparing surgery and platinum-based chemotherapy; in D group received fertility-sparing surgery and non-platinum-based chemotherapy. 5-year survival rate and prognostic factors were analyzed.Results:The overall 5- year survival rate was 69.2 %. The 5- years survival rates were 81.8 %, 40.0 %, 82.6% and 50.0% for A, B, C and D group, respectively. Platinum-based chemotherapy achieved significant survival benefit (82.4% vs. 44.4%, P0.05). Clinical stage, the size of residual tumor and Platinum-based chemotherapy were the mainly prognostic factors in multianalysis.Conclusions:Fertility-sparing surgery with a postoperative plainum-based chemotherapy should be the ideal modality for the treatment of yolk sac tumors of the ovary. Staging and tumor-reductive surgery were most associated with the prognosis of the patients.

Objective To investigate the effect of ulinastatin (UTI) on the expression of brainderived neurotrophic factor ( BDNF ) and remyelination in mice with experimental autoimmune encephalomyelitis ( EAE).Methods Twenty-four C57BL/6 mice were randomly divided into UTI group (U),normal saline treated group (S) and normal control group (N,n = 8,respectively).Demyelinations in the spinal cord were observed by solochrome cyanin staining.The expression of BDNF,myelin basic protein (MBP),and 2',3 '-cyclic nucleotide 3'-phosphodiesterase (CNP) in brain tissue of each group were evaluated by Western blot.Results Average clinical scores in group U at the 12,13,14,22,23,31,33,34 and 35 days were 0,0.25,0.38,0.63,0.63,0.40,0.40,0.40 and 0.40 respectively.They were significantly lower than group S at the same time ( U= 16.00,15.00,14.50,7.50,0.00,14.50,14.50,12.00 and 14.50,all P ＜0.05).Solochrome cyanin staining showed that demyelination of spinal cord in group U was also significantly improved than group S.Expressions of BDNF ( 1.96 ± 0.29),MBP (2.67 ± 0.48 ) and CNP ( 1.75 ± 0.20) in group U were all significantly higher than group S ( There were 0.80 ± 0.15,1.36 ± 0.38 and 1.06 ± 0.18 respectively,all P ＜ 0.05).Conclusions UTI has protective effect on EAE.The possible mechanism is that it could promote remyelination,and protect oligodendrocytes and neurons in EAE model by increasing BDNF expression in brain.

Objective To probe into the status and development trend of avian influenza,so as to provide reference for further research.Methods The article took the avian influenza literature as the object of study,took Gopubmed as the retrieval and statistical tool,made information analysis of the subjects,time,sources,distribution,authors and literature development trends of avian influenza research.Results The main subjects of avian influenza literature involved influenza in birds and influenza,human.There has been a sharp increase on the literature number of avian influenza in the recent decade with the most in 2006.The United States accounted for the most literature and China ranked the second place.The sources of avian influenza literature were mainly the SCI journals from the United States and European countries.Conclusions Gopubmed can show a vivid picture of the current situation and development trend of avian influenza research.Further improvements are needed both in the quality and quantity of avian influenza research in China

Objective To analyze the complete genome sequence of a Shenzhen coxsackievirus A2 strain CVA2-SHZH13-01 and its evolution.Methods RT-PCR was used to amplify the complete genome of CVA2-SHZH13-01 strain.The PCR products were purified and sequenced to analyze their genetic character-istics.Results The complete genome of CVA2-SHZH13-01 strain was 7400 bp in length, encoding 2191 amino acids.CVA2-SHZH13-01 strain was highly similar with the novel recombinant CVA2-HK (431306) strain isolated from Hong Kong sharing the nucleotide homology of 98.3%, 98.8%, 99.0%, 99.2%, 98.8%and 98.9%in 5′UTR, P1 ( VP1 to VP4) , P2, P3, 3′UTR regions and complete genome, respec-tively.CVA2-SHZH13-01 strain was highly identical to the international standard strain CVA2-Fleetwood showing the homology of 81.6% in nucleotide sequences in P1 region, but closely associated with EV71-SHZH03 and EV71-GD2009 strains (82.8%-88.7%) in P2 and P3 regions.The phylogenetic analysis in-dicated that CVA2-SHZH13-01 strain belonged to the CVA2-HK (431306) variant.Data from analysis of amino acid in P1 region showed that there were three amino acid mutations in CVA2-SHZH13-01 strain including aa5L→F, aa666S→G and aa671T→I as compared with CVA2-HK (431306) strain.Conclusion CVA2-SHZH13-01 strain belonged to CVA2-HK (431306) variant.

Objective To probe into the status and development trend of ebola,so as to provide reference for further research.Methods This paper took the ebola literature as the object of study,took GoPubMed as the retrieval and statistical tool,and conducted information analysis of the subjects,time,sources,distribution,authors and literature development trends of ebola research.Results The main subjects of ebola literature involved ebola virus and hemorrhagic fever,ebola.There has been a sharp increase on the literature number of ebola in the recent two years,particularly in 2014.The majority of literature came from the United States,and Britain ranked the second.The sources of ebola literature were mainly the SCI journals published in the United States and British.Conclusions GoPubMed can provide a vivid picture of the current situation and development trend of ebola research.Further improvements are needed both in the quality and quantity of ebola research in China.

Objective:To investigate the effect and mechanism of transcription factor Foxp 3 on the proliferation and cell cycle of human lung adenocarcinoma cell line A 549.Methods:We knocked down the expression of Foxp 3 using siRNA.Foxp3 inhibition was detected by RT-PCR.Cell proliferation was detected by MTT.Cell cycle of A549 cells were detected by flow cytometry after the transfection of siRNA.Cell cycle-related checkpoint genes were filtered by RT-PCR.The regulation of Foxp3 on cell cycle-related checkpoint genes were detected by immunofluorescence and dual -luciferase reporter assay system.Results: The proliferation of A549 cells were inhibited after silencing Foxp3,and A549 cells were arrested in G0/G1 cycle.G1/S cycle checkpoint gene CCND1 was down regulated.Mechanism research show that Foxp 3 can regulate the expression of CCND 1 directly.Conclusion: Foxp3 can promote the proliferation of A549 cell line by up regulating G 1/S cycle checkpoint gene CCND 1.This provides a new target for the therapeutic targets of lung adenocarcinoma.

Objective To assess the prevalence of c-Kit and FLT3 gene mutations in core binding factor related acute myeloid leukemia (CBF-AML) and analyze the karyotype characteristics of the CBF-AML patients. Methods Mutations of c-Kit, FLT3-ITD and FLT3-TKD were detected by genomic DNA PCR and sequencing, and the karyotype changes were analyzed in 48 newly diagnosed CBF-AML patients. Results c-Kit aberrations were detected in 13(27.1 %) out of 48 patients, including 5 cases with exon 8 mutation and 8 cases with exon 17 mutation. c-Kit was more prominent in t(8;21) AML patients than in inv(16) AML patients [(33.3 %(9/27) vs 19.0 %(4/21), P<0.05]. Only 1 case (2.1 %) had FLT3-ITD mutation (FLT3-ITD+) and 3 cases (6.3 %) had FLT3-TKD mutation (FLT3-TKD +). Prevalence of RUNX1-RUNX1T1 with additional chromosome abnormality was as high as 25.9 %(7/27), in which sex chromosome elimination was the most common one, while prevalence of CBFβ-MYH11 with additional chromosome abnormality was low. Conclusion c-Kit gene mutations and RUNX1-RUNX1T1 additional chromosome abnormalities are common in patients with CBF-AML and would be helpful for individualized treatment studies.

Objective To explore the information distribution of PM2.5 Paper in Foreign Language.Methods This article took PM2.5 papers as research object,used GoPubMed as information search and statistical analysis tool to conduct information mining of PM2.5 literature,including subject,time,author,source,distribution and number of publications over time.Results There was steady development of PM2.5 literature published in foreign language from 2004 to 2012,dramatic increase during the year 2013 to 2015.The three top publishing years of PM2.5 literature were 2015,2014 and 2013.Main authors of such literature include Chow J,Watson J,Koutrakis P and so on.Main research countries include United States,China,Canada,etc.Big cities,like Beijing,Shanghai,Boston were main places conducted such PM2.5 research.Related articles mainly published in journals such as J Air Waste Manag Assoc,Environ Sci Technol,Sci Total Environ and so on.Six out of the top ten research journals have higher impact factors than the average level of all journals of SCI in 2014.Main research terminologies include particulate matter,air pollutants,particle size and so on.Conclusions Generally,the PM2.5 literature over the world has a high level academically and China plays a significant role in such research.