Objective To study the relationship between the retinal arteriosclerosis prevalence and related factors in in-service teachers from 3 universities. Methods The medical report of in-service teachers from 3 universities from July to October, 2012 were analyzed. Results and Conclusion 2680 cases were included, in which 520 cases (19.4%) were diagnosed as retinal arteriosclerosis. Retinal arteriosclerosis prevalence increased with age and cardiovascular risk factors. In the multi-factor logistic regression analysis, gender, age, hypertension,hyperlipidemia, impaired fasting glucose regulation, diabetes, hyperuricemia are independent risk factors for retinal arteriosclerosis