Humans ; Laryngeal Diseases ; surgery ; Laryngoscopes ; Polyps ; surgery ; Vocal Cords ; surgery

In this study, the psbA-trnH sequence was used as DNA barcoding to evaluate the accuracy and stability for identification of Bamboo shavings, Concretio Silicea Bambusae and their closely related species. 56 samples were collected and conducted the DNA extraction. Obtained sequences were assembled using the CodonCode Aligner V4.2, the genetic distances and NJ tree were computed and constructed using MEGA 5.0. The results shows that the maximum intra-specific K2P distances were less than the minimum inter-specific K2P distances. The NJ tree indicated that Bamboo shavings, Concretio Silicea Bambusae and their closely related species can be distinguished from each other clearly, except for Bambusa tuldoides Munro and Bambusa textilis, which perhaps attributed to their closely genetic relationship.

Objective To explore short-term effect of tonsillotomy on children′s obstructive sleep apnea hypopnea syndrome (OSAHS) with tonsil hypertrophy by Ellman double-frequency radio-frequency machine .Methods Totally 30 cases children′s OS-AHS with tonsil hypertrophy were had tonsillotomy by Ellman double-frequency radio-frequency machine .They were compared with 30 cases children′s OSAHS with tonsil hypertrophy by traditional tonsillectomy .Short-term effect of tonsillotomy were ana-lyzed by wound bleeding ,operation time ,postoperative pain ,difficulty eating ,PSG testing ,immune function ,growth and develop-ment .Results Postoperative symptom in 1 weeks and 6 months ,compared with the traditional tonsillectomy ,30 cases of OSAHS patients were found that there were less bleeding ,shorter operative time ,mild postoperative pain ,no difficulty in feeding ,improving sleep-disordered breathing ,no decline in immune function ,good growth and development .Conclusion Short-term effect of tonsillot-omy on children′s OSAHS with tonsil hypertrophy is good by Ellman double-frequency radio-frequency machine ,it is a minimally invasive ,safe and effective surgical method children OSA HS .

Objective To compare the differences of target-volume(PTV) coverage and organ at risk (OAR) protection between three dimensional conformal radiotherapy (3DCRT) and intensity-modulated radiotherapy(IMRT) for patients with pelvis metastasis of cervical cancer underwent radical hysterectomy and pelvic lymphadenectomy. To explore the optimal treatment methods for pelvis metastasis of cervical cancer.Methods 10 patients with pelvis metastasis of cervical cancer underwent radical hysterectomy and pelvic lymphadenectomy were selected for this study. The images scanned by CT were transferred to treatment planning system to generate 3DCRT and IMRT plans. The impacts of 3DCRT on PTV were compared with those of IMRT. Isodose line and dose volume histograms(DVH) were used to evaluate to the dose-distribution in PTV and OAR. Results For 95 % confidence interval, the margin from CTV to PTV was 1 cm. Conformal indexs (CIs) of PTV for 3, 4, 5 and 6 fields 3DCRT were 0.46, 0.67, 0.68 and 0.68, respectively. When beyond 4 fields, the advantage of adding fields was not significant. CIs of PTV for 5, 7, 9, 11 and 13 fields IMRT were 0.75, 0.83 0.84, 0.85 and 0.85, respectively. When beyond 9 fields, the advantage of adding fields was not significant. The maximum dose of the bowl and spine cord in IMRT plans were lower than that in the 3DCRT plans (P <0.05). Maximum dose of OAR had no significant differences (includingt the bone, recttum and bladder) between IMRT and 3DCRT plans. Conclusion For patients with pelvis metastasis of cervical cancer after radical surgery, 4 fields planning in 3DCRT and 9 fields planning in IMRT are feasible. At high dose levels, the IMRT plans can more significantly protect the bowl and spine cord and decrease the radiation volume of colorectal and urinary bladder at risk than 3DCRT, so IMRT may potentially diminish probability of the normal tissue complications.

Objective To investigate the curative effect of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and the method of the pretreatment choice on myelodysplastic syndrome (MDS).Methods 13 cases who were undergoing allo-HSCT including 10 HLA-matched, 2 HLA partially matched,and one umbilical cord blood transplanted patients were enrolled in this study. The infusion number of MNC and CD+34 cells is 6.92 (2.65-21.33)×108/kg and 4.47 (1.49-10.22) ×106/kg, respectively. Of the 13 cases,5 adopted TBI, fludarabine(Flud), and cyclophosphamide(Cy) pretreatment, 3 chose BU/Cy pretreatment, and 3 chose TBI and Cy pretreatment, 2 chose Ara-C+BU+Cy+VM26 pretreatment. In order to prevent GVHD, 2cases of HLA partially matched were treated with ATG, cyclosporin A (CsA), MTX and MMF aftertransplantation, while the others were treated with CsA and MTX only. Results 9 out of 13 cases achieved hematopoietic reconstruction completely. 4 died of complications. Conclusion The allogeneic hematopoietic stem cell transplantation is an efficient regimen to cure MDS. The pretreatment should adopt the individuation.

Objective To investigate the effect of the TinniTest tinnitus comprehensive diagnostic and therapeutic apparatus for treating tinnitus and to analyze its clinical application value .Methods The TinniTest tinnitus comprehensive diagnostic and thera-peutic apparatus was adopted to inquiring ,evaluating ,diagnosing ,testing on 100 tinnitus patients .On this basis the tinnitus shelter treatment and the psychology consultation treatment were performed .Results The tinnitus disability quantitative test table was used to evaluate 100 patients .The evaluation results showed 12 cases of level 1 ,34 cases of level 2 ,28 cases of level 3 ,18 cases of level 4 and 4 cases of level 5 .The evaluation results of these 100 cases were classified to the mild degree≤ level 2 and the moderate degree >level 3 .Then the tinnitus test was performed .Among the cases of mild degree tinnitus ,4 cases were completely cured ,26 cases were significantly effective ,14 cases were effective and 2 cases were invalid ,the effective rate was 95 .6% .However among the cases of severe degree tinnitus ,0 case was completely cured ,28 cases were significantly effective ,18 cases were effective and 8 cases were invalid ,the effective rate was 85 .2% and the total effective rate was 90 .0% ,the difference in the effects between the two groups had no statistical significance(χ2 =1 .99 ,P>0 .05) .No adverse reactions occurred in all 100 cases .Conclusion The TinniT-est tinnitus comprehensive diagnostic and therapeutic apparatus can provide the comprehensive and accurate evaluation and the sci-entific tests to the tinnitus patients ,which has the definite effect for treating subjective tinnitus and the same effects for both mild and sever tinnitus patients .

In this study, the ITS2 sequence was used to identify Pinelliae Rhizoma and its adulterants to ensure its market circulation, clinical effect and safety. All genomic DNA from 59 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and NJ tree were calculated using software MEGA 6.0. The length of the ITS2 sequence of Pinelliae Rhizoma was 251 bp. The intraspecific genetic distance was smaller than the interspecific ones;The NJ tree indicated that Pinelliae Rhizoma distinguished from its adulterants obviously. The results showed that the ITS2 sequence can be used to distinguish Pinelliae Rhizoma from its adulterants accurately and stably.

Objective:To study the status and relationship of mental health,internet addiction disorder,nociceptive behavior and sleep quality among college students,and to provide evidence for healthcare. Methods:A total of 2 868 college students were chosen from Lanzhou University and surveyed with questionnaires on the status of mental health, internet addiction disorder, nociceptive behavior and sleep quality. Results:Sleep quality and health risk behaviors in girls were better than boys (P<0.05) . Psychological state in medical students was best. Sleep quality score in engineering students was the highest. Health risk behaviors in science students had highest score. Internet addiction behavior in engineering students had the highest score. Mental health score in three or more students was less than that in junior students,while sleep quality and internet addiction behavior score was higher (P<0.01) . Conclusion:Mental health has a negative influence on nociceptive behavior and internet addiction disorder,while it has a positive effect on sleeping quality. A negtive correlation lies between sleeping quality and nociceptive behavior or internet addiction disorder. The departments should pay attention to the status of mental health,internet addiction disorder,nociceptive behavior and sleep quality among college students,and the appropriate prevention is necessary.

Objective To explore the functional orientation of township hospitals in developed areas to achieve goals of the ongoing health reform.Methods Two rounds of Delphi consultation was made among 18 experts from around the country to learn the degree of importance of every function.Results The experts' average seniority of service in their fields was 23.28±10.41 years.The positivity coefficients of the two-round consultation were 100％.Kendall' S coefficients of the two-round consultation were 0.226 and 0.265 (p＜0.01).These data are of statistical importance,suggesting consensus among these experts and the consultation meeting the needs of forecast.In these consultation,seven class-1 functions and and 27 class-2 functions were determined.Medical services and public health services account for similar proportion of work of township hospitals.Conclusion The functional orientation was feasible and forward looking,for reference of township hospitals in developed areas.

Psammosilene Radix is a famous miao national herb and it is rare and endangered nowadays. However, it is often confused with the root of Silene viscidula. In this study, the ITS2 regions was used to identify Psam-mosilene Radix and its adulterants. All DNA samples of Psammosilene Radix and its adulterants were extracted. The ITS2 regions were amplified and sequenced, and the final sequences were assembled using the CondonCode Aligner. The genetic distances were calculated by kimura 2-parameter (K2P) model and the Neighbor-Joining(NJ) phylogenetic trees were constructed using MEGA5.1.BLAST 1, the nearest distance and phylogenetic tree (NJ-tree)methods were used to assess the identification efficiency of the ITS2 region. Results indicated that the length of ITS2 sequences of Psammosilene Radix w 229 bp, the identification effficiency of ITS2 region using BLAST 1 was 100%;and the maximum intra-specific K2P distance were lower than the minimum inter-specific K2P distance. Additionally, the NJ tree based on ITS2 sequence indicated that Psammosilene Radix and its adul-terants could be distinguished clearly . In conclusion , the ITS2 region as DNA barcodes could identify Psammosi-lene Radix and its adulterants stably and accurately. Furthermore, the application of ITS2 barcode in the identifi-cation of TCM has a good prospective .