The FactorⅫ(FXII or a-Ⅻa)in Cohn's FIV was adsorbed by diato- mite,and then the FXⅡ(or ?-Ⅻa) was activated into ?-Ⅻa(Hageman factor fragment,PKA,MW31000)by the co-adsorbed kallikrein with sub- sequent releasing it toabuffer solu- tion.Finally,the product was purified by DEAE-Sephadex A_(50).The activity of prepared PKA was 8 963IU/ml,and the preparation can lead to signifi- cant reduction in blood pressure in rats.

Objective To investigate the efficacy of wax therapy plus functional training in treating ankylosing spondylitis. Method One hundred patients were randomly allocated to treatment and control groups, 50 cases each. The treatment group received wax therapy plus functional training and the control group, functional training alone. One course of treatment consisted of seven days in both groups. The therapeutic effects clinical were evaluated after two course of treatment. Result Joint pain severity and morning stiffness time improved in both groups after treatment (P＜0.05 or P＜0.01) and did more in the treatment group than in the control group (P＜0.01). The total efficacy rates in the two groups were 84.0%and 66.0%, respectively;there was a statistically significant difference between the two groups (P＜0.05). Conclusion Wax therapy plus functional training is safe, reliable, convenient and easy to perform in clinical treatment of ankylosing spondylitis.

In the paper, a microtiter plate method was used to determine the residual prekallikrein activator(PKA) activity in blood products.The result of study indicated that it would be satisfactory that the reaction mixture of PKA and prekallikrein (PK) was iucul ?? at room temperature for 30 minutes with 0.05M Tris/0.15M NaCl buffer(pH 8.0) , and then the mixture continued to be incubated at room temperature for another 15 minutes after the chromogenic substrate for kallikrein was added. The method had a good reproducibility. The PKA contents determined in IVIG agreed with that of the processing method of IVIG and the hypotensive test of rats, and were basically consistent with the result, determined by the method developed by the Finnish Red Cross Blood Transfusion Service.

Objective To analyze the validity of clinical memory scale in patients with Parkinson's disease (PD). Methods 60 PD patientswere assessed by clinical memory scale and Mini-Mental State Examination (MMSE). Results The scores of clinical memory scalesignificantly correlated with the scores of MMSE (r=0.608, P<0.001). The scores of clinical memory scale were higher in no-memory impairmentgroup than in memory impairment group (P<0.05). Conclusion Clinical memory scale is good at evaluating the memory impairmentof PD patients with good criteria validity and discriminating validity.

Objective To study the characteristic of memory impairment and its related factors in patients with Parkinson's disease (PD). Methods 155 PD patients were assigned to 4 age groups. Clinical memory scale, Hamilton Rating Scale for Depression (HAMD) and Hamilton Rating Scale for Anxiety (HAMA) were used to assess them. Results The memory quotient (MQ) of PD patients were significantly lower than the norm (P<0.01). The scores of associational learning, direct memory, picture recall and portrait association in each age groups were lower than norm (P<0.05), but nonsense figure recognition (P>0.05). The MQ of PD patients was significantly related with education and depression or anxiety (P<0.01), and was not correlated with age, gender and course of disease (P>0.05). Conclusion The MQ of PD patients are lower especially in associational learning, direct memory, picture recall and portrait association. The MQ of PD patients is correlated with education and depression or anxiety significantly.

General practitioner represents the core competency of community health care center and also the key for sustainable discipline development.Talent developing is regard as critical issue for both discipline construction and community health service development.Community health care center,as a platform for internship,practice and continue medical education of medical professionals,plays an important role in career development of general practitioner.We clearly defined the role and responsibility of community health care center in general practicing talent development,and built an excellent career development platform.We integrated project-talent-discipline three-in-one to promote clinical research and talent development;applied two-factor theory to set up effective talent plan and incentive mechanism.This article summarized our approach and experience in talent development as a reference for colleagues in other community centers.

Objective To investigate the status of life quality in the elderly in Shanxi Province Changzhi City, and to analyze its influencing factors. Methods The quality of life, activities of daily living and status of community services were measured simultaneously by 36-Item Short Form Health Survey (SF-36). Activities of Daily Living Scale, Community Healthy Needs Scale and survey results were used and analyzed using t-test, correlation and stepwise regression analysis. Results According to the criterion of life quality in the elderly which were proposed by Zhang Lei, the quality of life in the elderly scored 72. 1-117.0, reached 98.5%. The quality of life in the elderly was impacted by the ability of caring oneself, marital status, degree of culture, economic situation and so on. The demand rate for health guidance and periodic physical examination was higher. Conclusions The quality of life in the elderly is at a medium level. There is a wide gap between the demand of community healthy services and the utilization of community healthy services.

Objective To investigate the quality of life of elderly caregivers and its factors. Methods Elderly caregivers living in Changzhi City were enrolled in this study. 36-item short-form (SF-36) health survey and Activity of Daily Living Scale ( ADL) were used as study tools. The data was analyzed by statistical description,t test or multiple regression. Results Those with score of quality of life reaching 72. 1 to 117. 0 accounted for 98. 8%. The total score and score of eight dimensions of SF-36 showed statistically significant difference between different daily living activity groups ( P < 0.05) . The state of health and self-care ability of the elderly imposed a major impact on eight dimensions of SF-36. Age, sex, level of education, marital status and wage had different impact on each dimension of SF-36. Conclusion The quality of life of elderly caregivers is at a medium level. The main factors of quality of live of elderly caregiver are their state of health and self-care ability of the elderly.

BACKGROUND:Neuromuscular junction structure has defects in patients with Duchenne muscular dystrophy, mainly presenting acetylcholine receptor structure fragmentation and the reduction of spine-like processes on thepostsynaptic membrane. It is generaly recognized that the structural defects are induced by structural damage of muscle cels and muscle fiber necrosis. OBJECTIVE:To explore the reasons of damage on neuromuscular junction in mouse models of Duchenne muscular dystrophy. METHODS: We introduced Duchenne muscular dystrophy models of male mdx mice and male Dko mice. After gene identification, they were used for tests. Male C57BL/6 mice were selected as normla controls. Hematoxylin-eosin staining was utilized to detect pathological changes in muscles. Neuromuscular junction structure was revealed using immunofluorescence staining. The differences in dystrophin expression, pathological features and neuromuscular junction structure were compared in mouse models of two kinds of Duchenne muscular dystrophy. RESULTS AND CONCLUSION:The introduced mouse models were accorded with the requirement of our experiment in aspects of genotype and protein expression levels. The number of acetylcholine receptor was apparently reduced in the neuromuscular junction of two kinds of mouse models. Although dko mouse muscles presented more obvious inflammatory infiltration and muscle fiber damage compared with mdx mice, but there was no significant difference in the damage to neuromuscular junction between them, and acetylcholine receptor fragmentation was identical. The evidence suggested that structural damage of neuromuscular junction and inflammatory pathological response are independent events. There is no direct relationship between them. Dystrophin gene deficiency is the main cause of the fragmentation of the acetylcholine receptor.